Identi cation of Gh|Alu-I Gene Polymorphisms in Indonesian Simeulue Buffalo



Eka Meutia Sari(1*), Mohd. Agus Nashri Abdullah(2), Mohd. Yunus(3), Elmy Mariana(4), Eryk Andreas(5)

(1) Faculty of Agriculture, Dept. of Animal Science, Syiah Kuala University, Banda Aceh 23111
(2) Faculty of Agriculture, Dept. of Animal Science, Syiah Kuala University, Banda Aceh 23111
(3) Faculty of Agriculture, Dept. of Animal Science, Syiah Kuala University, Banda Aceh 23111
(4) Faculty of Agriculture, Dept. of Animal Science, Syiah Kuala University, Banda Aceh 23111
(5) Faculty of Animal Science, Bogor Agricultural University, Jl. Agatis, Darmaga Campus, Bogor 16680
(*) Corresponding Author

Abstract


The purpose of this study was to identify the GH/AluI gene polymorphism in Indonesian Simeulue buffalo. To the best of our knowledge this is the rst published data on the polymorphism of growth hormone (GH) gene in Simeulue buffalo. The 178 DNA samples buffalo were collected from three districts in Simeulue Island, Teupah Selatan (71), Teupah Barat (59), and Salang (48). Result shows that a gene fragment of the GH|AluI gene at 432 bp located on exon 3 were successfully ampli ed by using the techniques of PCR (polymerase chain reaction) and genotyped by PCR-RFLP (restriction fragment length polymorphism). Based on that results showed no polymorphisms were detected in these genes. All buffaloes tested had LL genotype for locus GH|AluI. 


Keywords


Simeulue Buffalo, Growth Hormone Gene, Polymorphism

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