Supplementing Energy and Protein at Different Degradability to Basal Diet on Total Protozoa and Microbial Biomass Protein Content of Ongole Grades Cattle

Abstract

This research aimed to determine energy and protein proportion as feed supplement on total protozoa (TP) and microbial biomass protein (MBP) in the rumen of cattle fed by basal diet of corn ear and coffee pods. In vivo research was done at barn and laboratory of Animal husbandry faculty of GMU. As of four stulated Ongole Grades Cows (aged I2, live weight 250 + 15 kg) were used. Animal were placed into individual pens. Research was held in four period (P1, P2, P3 and P4), each period consisted of 10 days including adaptation. Feed was the basal diet (BF) and supplement at ratio of 60:40 (3% of LW). At P1= animal-1 fed by BF without supplement (feed A), animal-2 fed by BF supplemented by HDE+LDP (feed B), animal-3 fed by BF supplemented LDE+LDP (feed C) and animal-4 fed by BF supplemented HDE+HDP (feed D). Within P2, P3 and P4, simultaneously animals were received the same feed. Feed was given two times a day and water was given ad libitum. Rumen uid was taken at 0700, 1200, and 1600. Determination of TP according to Diaz et al., (1993). Meanwhile the Lowry methods was followed by to evaluate MBP. The data of each sample were analysed by One way analysis of variance using SPSS program ver13.0. Result showed that amount of TP at before feeding (0700) was vary from 17.3 to 47.7 cell x 103/ml. Within feed treatments was no signi cant different, but it can be indicated that the high increase of amount of TP was occur at four hours after feeding time. The feed treatments was also had same yield of MBP which was vary from 195.21 mg/ml to hingga 297.84 mg/ml. The highest of MBP yields was at BF + (HDE+HDP) (297.84 mg/ml), followed by BF + (LDE+LDP) (269.56 mg/ml), BF + (HDE+LDP) (215.59 mg/ml), and BF (189.25 mg/ml). There was indicated that supplementing energy and protein source of high degraded at basal diet had the best response to the amount of TP and MBP.