Effect of Extender Medium and Cooling Rate on the Quality of Frozen Semen Post-Thawing at Bali Bull (Bos sondaicus)

Abstract

This study was aimed to analyze effect of extender medium and cooling rate on the quality of frozen semen post-thawing  at Bali bull (Bos sondaicus). The experiment was conducted in a completely ramdomized design factorial of 6 threatment (2x3) and 3 replicates. Data were statistically analyzed using analysis of variance (ANOVA) and Duncan test was employed to assess statistical differences between treatment. The first factors was extender such as Tris citrat egg yolk (TCEY) and Tris citrat soy milk  (TCSM). The second factors was the cooling rate such as: 15°C/min, 10°C/min and 5°C/min. Variables measured include: sperm motility, live sperm, sperm abnormality, and membrane integrity of spermatozoa Bali bull. The results of this study on the treatment of extender medium TCEY (A1) shows the best results with the sperm motility of 43.15 ± 4.67%, live sperm 53.78 ± 3.79%, sperm abnormality 18.89 ± 1,07% and membrane integrity 35.44 ± 3.01%. As well as the results of research on the treatment with cooling rate of 10°C /min (B2) showed the best results with sperm motility of 44.35 ± 5.28%, live sperm 57.17 ± 1.18%, sperm abnormality of 17.84 ± 0.23 % and membrane integrity 36.83 ± 2.12%. The interaction between extender medium and cooling rate was founded significant different (P>0.05). Results of analysis of variance showed that there was a highly significant difference (P> 0.01) between the two extender medium and three cooling rate on post - thawed of  motility, live sperm and membrane integrity and had no significant (P <0.05) on the sperm abnormality, It can be concluded that the extender medium Tris-citrat egg yolk (TCEY) with cooling rate of 10°C / min resulted the best in sperm motility, live sperm, and membrane integrity spermatozoa.