Genetic Variation of Muscovy Ducks MC1R Gene in a Different Feather Colors Population

Abstract

This research was aimed at gene sequence variations in the melanocortin 1 receptor (MC1R) and genotype relationships with different groups of feather colors in Muscovy ducks. Two hundred Muscovy days old ducks consisted of white feather male and female duck, black-white combination color male and female ducks. Primary design used Custal X, based on database from GeneBank Cairina moschata GH gene, partial cds (KX013541.1), primary Forward sequence: 5'-GGACCGCTACATCACCATCT-'3 and Reverse Primer: 5'-TGTAGAGCACCAGCATGAGG-'3. The results of the identification of feather color of Muscovy ducks shown there are variation on white color, white-black combination with white dominance and white-black color combination with black dominance. Variations of the feather color on the head, wings, breast, tail and plumage. The sequencing of PCR products obtained nucleotide polymorphism. GG genotype was observed in 293 nt only on white-black, CC in white-black and white feather color of male and female Muscovy ducks. Conclusively, Muscovy ducks had variation feather color, it was white and white-black combination. MC1R gene polymorphism was observed in Muscovy duck.