Detection of Aeromonas hydrophila infection on Tilapia fish (Oreochronis niloticus) skin by Hematoxillin-Eosin and immunohistochemistry staining

Yuli Purwandari Kristianingrum(1*), Quek Chuan Cong(2), Aris Haryanto -(3)

(1) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(2) Chop Cheong Bee, Sdn Bhd, Malaysia
(3) Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jln. Fauna 2, Karangmalang Yogyakarta 55281, Indonesia.
(*) Corresponding Author


Aeromonas hydrophila infection has caused major losses in fish industries in Indonesia. An accurate diagnosis of the diseases caused by A.hydrophila is needed in order to increase the effectiveness of the treatment. The aim of this research was to observe the histopathological changes in Tilapia fish skin caused by A.hydrophila infection. Five A.hydrophila-infected tilapia fish skin samples with hemorrhagic and ulceration lesions were made into histopathologic specimen by using H-E staining. Immunohistochemistry staining was applied on the skin samples by using primary antibody obtained from rabbit and secondary antibody with HRP-System. Afterwards, IHC staining was done to further confirm the diagnosis of A.hydrophila infection. The histopathological findings of A.hydrophila on Tilapia fish skin tissue samples were focal hemorrhagic lesions and infiltration of neutrophils. All 5 tissue samples were tested positive for A.hydrophila in the immunohistochemistry staining, which indicated brown color on the tissue. This research concluded that the histopathological changes in A.hydrophila-infected fish skin included hemorrhages and neutrophils infiltration. The diagnosis was further confirmed by the brown color indicated in the immunohistochemistry staining

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