Uji Aktivitas Antiplatelet Bromelain Secara In Vitro Menggunakan Spektrofotometer UV-Vis

https://doi.org/10.22146/ijl.v5i2.76068

Lilis Wijayanti(1*), Suryani Suryani(2), Rachmadi Wibowo(3)

(1) UIN Syarif Hidayatullah Jakarta
(2) UIN Syarif Hidayatullah Jakarta
(3) 
(*) Corresponding Author

Abstract


Salah satu penyebab beberapa penyakit kardiovaskuler adalah adanya sumbatan pada pembuluh kapiler darah yang disebabkan agregasi platelet dan reaksi aterotrombosis. Akan tetapi, obat-obatan antiplatelet masih memiliki efek samping. Enzim Bromelain dilaporkan memiliki aktivitas antiplatelet yang potensial. Oleh karena itu, penelitian ini bertujuan untuk mengetahui aktivitas enzim Bromelain dalam menghambat agregasi platelet secara in vitro. Aktivitas antiplatelet ditentukan dengan mengukur persen agregasi platelet dalam Platelet Rich Plasma (PRP) darah manusia setelah diinduksi dengan Adenosin Difosfat (ADP) menggunakan Spektrofotometer Uv-Vis. Penelitian ini menggunakan akuades sebagai kontrol negatif, asetosal sebagai kontrol positif, dan enzim Bromelain hasil dialisis sebagai sampel uji. Berdasarkan penelitian ini dapat disimpulkan bahwa enzim Bromelain hasil dialisis dapat menghambat agregasi platelet yang diinduksi ADP yaitu sebesar 76,59%.


Keywords


Kardiovaskular; bromelain; antiplatelet; asetosal

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References

Fitria, Y. N., Budianto, E., & Setiasih, S. (2018). Encapsulation efficiency and dissolution of bromelain from chitosan-poly(N-vinylpyrrolidone) semi-IPN hydrogel. AIP Conference Proceedings, 2049(December). https://doi.org/10.1063/1.5082436

Hassan, A and Othman, Z. (2011). Pineapple [Ananas comosus (L.) Merr. In Pineapple (Ananas comosus L. Merr.) (pp. 194–218). Woodhead Publishing Limited. https://doi.org/10.1007/978-1-4939-1658-0_23

Herfena, N., Setiasih, S., Handayani, S., & Hudiyono, S. (2020). Evaluation of in-vitro dissolution profiles of partially purified bromelain from pineapple cores ( Ananas comosus [ L .] Merr ) loaded in glutaraldehyde-crosslinked chitosan microspheres Evaluation of in-vitro dissolution profiles of Partially Purified Br. AIP Conference Proceedings.

Hilberg, T., & Gla, D. (2006). The influence of bromelain on platelet count and platelet activity in vitro. Platelets, 17(February), 37–41. https://doi.org/10.1080/09537100500197489

Metzig, C., Grabowska, E., Eckert, K., Rehse, K., & Maurer, H. R. (1999). Bromelain proteases reduce human platelet aggregation in vitro, adhesion to bovine endothelial cells and thrombus formation in rat vessels in Vivo. In Vivo, 13(1), 7–12.

Pavan, R., Jain, S., Shraddha, & Kumar, A. (2012). Properties and Therapeutic Application of Bromelain: A Review. Biotechnology Research International, 2012, 1–6. https://doi.org/10.1155/2012/976203

Putranto, M. A., Budianto, E., & Hudoyon, S. (2018). Encapsulation and dissolution study of bromelain in chitosan-methyl cellulose semi-IPN hydrogel. AIP Conference Proceedings, 2049. https://doi.org/10.1063/1.5082438

Putri, R. R. R. F., Ulfa, E. U., & Riyanti, R. (2014). Uji Aktivitas Antiplatelet Ekstrak Etanol Kubis Merah (Brassica oleracea var. capitata L.) Antiplatelets activity of red cabbage ethanolic extract (Brassica oleracea var. capitata L.). Pustaka Kesehatan, 2(1), 111–114. http://jurnal.unej.ac.id/index.php/JPK/article/view/605

Rohmah, M. K., & Fickri, D. Z. (2020). Uji Aktivitas Antiplatelet , Antikoagulan , dan Trombolitik Alkaloid Total Daun Pepaya ( Carica papaya L .) secara in Vitro. Jurnal Sains Farmasi & Klinis, 7(2), 115–125. https://doi.org/10.25077/jsfk.7.2.115-125.2020

Sandhy, P. P., Budianto, E., & Hudiyono, S. (2018). Bromelain encapsulation on hydrogel chitosan-poly(N-vinyl pirrolidone) full-IPN. AIP Conference Proceedings, 2049(December). https://doi.org/10.1063/1.5082441

Silva-lópez, R. E., & Natashe, R. (2019). Therapeutic proteases from plants : biopharmaceuticals with multiple applications. Journal of Applied Biotechnology & Bioengineering, 6(2), 101–109. https://doi.org/10.15406/jabb.2019.06.00180

W A Hidayani, S. S. and S. H. (2020). Determination of the effect of EDTA and PCMB on purified bromelain activity from pineapple core and in vitro antiplatelet activity. I O P Conference Science, Materials Science and Engineering, 763. https://doi.org/10.1088/1757-899X/763/1/012054

Wirawan, R. (2007). Nilai Rujukan Pemeriksaan Agregasi Trombosit dengan Adenosis Difosfat pada Orang Indonesia Dewasa Normal di Jakarta. Majalah Kedokteran Indonesia, 57(7), 212–219.

Yunita, E. P., Zulkarnain, B. S., & Aminuddin, M. (2015). Aspirin Resistance in Coronary Artery Disease with Hypertensive Patients. Indonesian Journal of Clinical Pharmacy, 4(1), 28–38. https://doi.org/10.15416/ijcp.2015.4.1.28



DOI: https://doi.org/10.22146/ijl.v5i2.76068

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