Editorial Board Member: Prof. Kazuhito Fujiyama

Curriculum Vitae

 

 

Contact address

International Center for Biotechnology (ICBiotech), Osaka University, 2-1 Yamaoka-oka, Suita, Osaka 565-0871, Japan

Education
1990 PhD (supervised by Prof. Hirosuke Okada and Prof. Atsuhiko Shinmyo)
1984-1986 Master course, Department of Fermentation Technology, Graduate School of Engineering, Osaka University
1980-1984 Department of Fermentation Technology, Faculty of Engineering, Osaka University
   
Employment 
2017-Now Director, International Center for Biotechnology, Osaka University
2009-Now Professor, International Center for Biotechnology, Osaka University
2003-2009 Associate Professor, Center for Biotechnology, Osaka University
1988-2003 Assistant Professor, Center for Biotechnology, Osaka University
   
Fellowships  
2004-Now The Biodesign Institute at Arizona State, Non-Resident Research Faculty (Adjunct Faculty)
2003-2004        Visiting scientist, Arizona State University (Dr. L. Joshi)
1999 Visiting scientist, Universtiy of Zurich (Dr. E. Berger)
1998 Visiting scientist, Boyce Thompson Institute for Plant Research at Cornell (Dr.C.J. Arntzen)
1988 Visiting scientist, Univesity of California at Davis (Dr.R.L. Rodriguez)
   

Short Overview about Scienttific Work

Glyco-engineering of hetelogously-produced recombinant proteins, Plant glycobiology, Applied microbiology

Others

2019   Honorary Professor, Institut Teknologi Bandung, Indonesia (Oktober 18)

Key Publications

Heterologous fusion gene expression and characterization of a novel carbohydrate binding module (Cbm36) to laccase (Lcc2) Puspaningsih, Kartika Dwi Asni Putri, Mamik Damayantia, Ni Nyoman Purwani, Sylvia Aulia Rahmah, Purkan, Kazuhito Fujiyama, Makiko Sakka, Kazuo Sakka, Tetsuya Kimura, Ali Rohman, Afaf Baktir, Rahmat Eko Sanjaya. Biocatalysis and Agricultural Biotechnology

Temporal analysis of N-acetylglucosamine extension of N-glycans in the middle silk gland of silkworm Bombyx mori Kajiura H, Eguchi T, Uchino K, Tatematsu KI, Tamura T, Sezutsu H, Fujiyama K. J Biosci Bioeng. 2022 Apr 6:S1389 1723(22)00062-7

Evaluation of homogeneity of plant-derived functional proteins; effect of fruit maturation on N-glycosylation of native and recombinant miraculin. Kajiura H, Hiwasa-Tanase K, Ezura H, Fujiyama K. Plant Physiol Biochem. 2022 May 1;178:70-79

Production of recombinant β-glucocerebrosidase in wild type and glycoengineered transgenic Nicotiana benthamiana root cultures with different N-glycan profiles Uthailak N, Kajiura H, Misaki R, Fujiyama K. J Biosci Bioeng. 2022 May;133(5):481-488

Analysis of N-glycan profile of Arabidopsis alg3 cell culture Sariyatun R, Kajiura H, Limkul J, Misaki R, Fujiyama K. Plant Biotechnol (Tokyo). 2021 Dec 25;38(4):463-467

Establishment of suspension-cultured Chinese hamster ovary cells with double knockout of GDP-mannose-4,6-dehydratase and GDP-fucose transporter Misaki R, Iwasaki M, Takechi H, Yamano-Adachi N, Ohashi T, Kajiura H, Fujiyama K. Cytotechnology. 2022 Feb;74(1):163-179

Improved Assay System for Acidic Peptide: N-glycanase (aPNGase) Activity in Plant Extracts Yamamoto C, Ogura M, Uemura R, Megumi M, Kajiura H, Misaki R, Fujiyama K, Kimura Y. Anal Biochem. 2021 Dec 1;634:114367

Takao Ohashi, Nabilah Sari, Ryo Misaki, Kazuhito Fujiyama. Biochemical characterization of Arabidopsis clade F polygalacturonase shows a substrate preference toward oligogalacturonic acids. J Biosci Bioeng. 2021 Oct 21:S1389-1723(21)00210-3. doi: 10.1016/j.jbiosc.2021.08.007.

Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture. Sariyatun R, Florence, Kajiura H, Ohashi T, Misaki R, Fujiyama K. Front Plant Sci., 2021 Jul 14;12:703020.

Transient Production of Human β-Glucocerebrosidase with Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants Uthailak N, Kajiura K, Misaki R, Fujiyama K. Front Plant Sci. 2021 Jun 7;12:683762.

Shirai S, Uemura R, Maeda M, Kajiura H, Misaki R, Fujiyama K, Kimura Y. Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: in vitro assay system for plant cPNGase activity. Biosci Biotechnol Biochem. 2021 May 25;85(6):1460-146

Functional characterization and overexpression of Δ12-desaturase in the oleaginous yeast Rhodotorula toruloides for production of linoleic acid-rich lipids. Wu CC, Ohashi T, Kajiura H, Sato Y, Misaki R, Honda K, Limtong S, Fujiyama K. J Biosci Bioeng. 2021 Jun;131(6):631-639

Bombyx mori β1,4-N-acetylgalactosaminyltransferase possesses relaxed donor substrate specificity in N-glycan synthesis. Kajiura H, Miyauchi R, Kakudo A, Ohashi T, Misaki R, Fujiyama K. Sci Rep. 2021 Mar 9;11(1):5505

Structure-function studies of ultrahigh molecular weight isoprenes provide key insights into their biosynthesis Kajiura H, Yoshizawa T, Tokumoto Y, Suzuki N, Takeno S, Jumtee-Takeno K, Yamashita T, Tanaka S, Kaneko Y, Fujiyama K, Matsumura H, Nakazawa Y. Commun Biol. 2021 Feb 16;4(1):215

Kinetic and Thermodynamic Study of Plantaricin IIA-1A5, a Bacteriocin Produced by Indonesian Probiotic Lactobacillus plantarum IIA-1A5. Arifin M, Budiman C, Fujiyama K, Arief II. Protein Pept Lett. 2021;28(6):680-686

Ohashi T, Fujisawa Y, Hayes MR, Misaki R, Pietruszka J, Fujiyama K. Transglycosylation toward naringenin-7-O-glucoside using an N180H mutant of Coprinopsis cinerea endo-β-N-acetylglucosaminidase. Biochem Biophys Res Commun, 2020 September 10; 530(1): 155-159

Kajiura H, Nakamura Y, Nishimura M, Ohashi T, Misaki R, Fujiyama K. Characterization of Bombyx mori N-acetylglucosaminyltransferase II splicing variants. Biochem Biophys Res Commun, 2020 August 20;529 (2): 404-410

Takuhiro Matsumura, Sho Amatsu, Ryo Misaki, Masahiro Yutani, Anariwa Du, Tomoko Kohda, Kazuhito Fujiyama, Kazuyoshi Ikuta, Yukako Fujinaga. Fully Human Monoclonal Antibodies Effectively Neutralizing Botulinum Neurotoxin Serotype B. Toxins (Basel). 2020 May 7;12(5):302