Polymorphism of Transcription Factor 7-Like 2 Gene and HOMA-β Level of Individuals With and Without Type 2 Diabetes Mellitus Family History
https://doi.org/10.22146/ijbiotech.9312 Waode Astria Sahrani(1*), Indwiani Astuti(2), Ahmad Hamim Sadewa(3)
(1) Master Program of Basic Medical Science and Biomedic, Molecular Medicine Department, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
(2) Department of Pharmacology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
(3) Department of Biochemistry, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
(*) Corresponding Author
Abstract
Family history has considered as a risk factor of type 2 diabetes. Transcription factor-7 like 2 (TCF7L2) has
role to regulates insulin secretion and blood glucose homeostasis. The aim of current study was to determine the
rs7903146 polymorphism of TCF7L2 gene and homeostatic model assessment-β (HOMA-β) level on individual with
and without type 2 Diabetes Mellitus (DM) family history. This work is a case-control study. Thirty six subjects
with type 2 DM family history and 36 subjects without type 2 DM family history were recruited. HOMA-β
measure to analyze the insulin secretion. Polymorphisms of TCF7L2 gene was analyzed by using PCR-RFLP
method. Statistical analysis was performed by using T-test, Mann-Whitney and Chi-square with signifi cance
level 0.05. The frequency of the T allele of the cases were 4.2% and the controls were 2.8% (p=0.500). The odd
ratio was 0.649 (CI;95%:0.106-4.055). The HOMA-β levels of the cases were signifi cant low (132.56±62.48)
compared with the controls (266.09±1.68) with p=0.000. The subjects with type 2 DM family history have a
similar frequency of having T alleles and CT/TT genotypes. The subjects with type 2 DM family history has
signifi cantly lower HOMA-β levels than subject without DM family history.
role to regulates insulin secretion and blood glucose homeostasis. The aim of current study was to determine the
rs7903146 polymorphism of TCF7L2 gene and homeostatic model assessment-β (HOMA-β) level on individual with
and without type 2 Diabetes Mellitus (DM) family history. This work is a case-control study. Thirty six subjects
with type 2 DM family history and 36 subjects without type 2 DM family history were recruited. HOMA-β
measure to analyze the insulin secretion. Polymorphisms of TCF7L2 gene was analyzed by using PCR-RFLP
method. Statistical analysis was performed by using T-test, Mann-Whitney and Chi-square with signifi cance
level 0.05. The frequency of the T allele of the cases were 4.2% and the controls were 2.8% (p=0.500). The odd
ratio was 0.649 (CI;95%:0.106-4.055). The HOMA-β levels of the cases were signifi cant low (132.56±62.48)
compared with the controls (266.09±1.68) with p=0.000. The subjects with type 2 DM family history have a
similar frequency of having T alleles and CT/TT genotypes. The subjects with type 2 DM family history has
signifi cantly lower HOMA-β levels than subject without DM family history.
Keywords
Type 2 DM family history, TCF7L2 gene, rs7903146 polymorphism, HOMA-β
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PDF DOI: https://doi.org/10.22146/ijbiotech.9312
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