In Vitro Screening of Falcataria moluccana (Miq.) with Gall Rust (Uromycladium tepperianum (Sacc.) Filtrate as Media Selection

Abstract

In vitro screening of Falcataria moluccana (Miq.) was conducted by tissue culture method. Seeds from
two different site of community forest, 400 m (S1) and 800 m (S2) above sea level, were used as material.
Double concentration of MS (Murashige & Skoog, 1962) with 40 mg/l gall rust (Uromycladium tepperianum
(Sacc.) fi ltrate were used for media selection. The results of this research showed that 66 % axenic plantlets in
vitro from S1 and 27 % from S2 were still survived after 3 months incubation without subculture. The mean
of fresh weight (2. 21 ± 0. 26 g) and dry weight (1. 97 ± 0. 12 g) from S1 plantlets lower than the mean of fresh
weight (2. 87 ± 0,18 g) and dry weight (2. 16 ± 0. 14 g) from S2 plantlets. Qualitative of terpenes, saponins and
quantitative of total phenolics were analyzed from those gall rust extract, as source of fi ltrate media, attacked
and un-attacked of F. moluccana. They all qualitatively have capability to produce terpenoid and saponin. It
is notice that U. tepperianum, un-cultured pathogen, contain of those compound that may play a role as codeterminants
of pathogenecity. While the highest total phenolic compound were contained in gall rust extract
(2. 35 %), followed by attacked F. moluccana branches (1. 18 %) and un-attacked F. moluccana branches (0. 44
%). This indicated that phenolic compound in gall rust has higher activity as a response of F. moluccana to U.
tepperianum pathogen pressures and result of this study suggest the great value of gall rust fi ltrate for use as
media selection in vitro.