Effect of Culture Medium Supplementation with b-mercaptoethanol and Amino Acid on Canine Intergeneric Embryo Development with Porcine Oocyte Cytoplasm Recipient
https://doi.org/10.22146/ijbiotech.7764 Yuda Heru Fibrianto(1*)
(1)
(*) Corresponding Author
Abstract
The present study investigated the effect of culture medium supplementation with mercaptoethanol ( ME)
and amino acid (AA) on canine intergeneric embryo development with porcine oocyte cytoplasm. Porcine cumulus
oocyte complexes (COCs) were collected from slaughterhouse and matured in TCM-199 supplemented with 26.2
mM NaHCO, 3.05 mM glucose, 0.91 mM sodium pyruvate, 0.57 mM L-cysteine, 75 mg/l kanamycin, 10 ng/ml 3
epidermal growth factor, equine chorionic gonadotropin (eCG), 10 IU/ml human chorionic gonadotropin (hCG),
and 10% (v/v) porcine follicular fluid (pFF) at 39 °C in a humidified atmosphere of 5% CO for 42-44 h and donor cell 2
collected from ear skin afghanhound male dog. After somatic cell nuclear transfer (SCNT), embryo development
were examined for cleavage rate and 144 hr for final development after cultured in media. The result shows that,
amino acid and mercapoethanol addition in culture medium (NCSU-23) have no effect on embryo development.
The development rate of embryo until 16 cell stage in NCSU and NCSU supplement are 4.67% and morula stage are
3.73% and 4.67%.
Key words : intergeneric clone embryo, canine, ( amino acid (AA)
and amino acid (AA) on canine intergeneric embryo development with porcine oocyte cytoplasm. Porcine cumulus
oocyte complexes (COCs) were collected from slaughterhouse and matured in TCM-199 supplemented with 26.2
mM NaHCO, 3.05 mM glucose, 0.91 mM sodium pyruvate, 0.57 mM L-cysteine, 75 mg/l kanamycin, 10 ng/ml 3
epidermal growth factor, equine chorionic gonadotropin (eCG), 10 IU/ml human chorionic gonadotropin (hCG),
and 10% (v/v) porcine follicular fluid (pFF) at 39 °C in a humidified atmosphere of 5% CO for 42-44 h and donor cell 2
collected from ear skin afghanhound male dog. After somatic cell nuclear transfer (SCNT), embryo development
were examined for cleavage rate and 144 hr for final development after cultured in media. The result shows that,
amino acid and mercapoethanol addition in culture medium (NCSU-23) have no effect on embryo development.
The development rate of embryo until 16 cell stage in NCSU and NCSU supplement are 4.67% and morula stage are
3.73% and 4.67%.
Key words : intergeneric clone embryo, canine, ( amino acid (AA)
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PDF DOI: https://doi.org/10.22146/ijbiotech.7764
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