Developmental Competence of Early Stage Porcine Embryos Cultured in Medium with Different Energy Substrate in vitro

Ni Wayan Kurniani Karja(1*), Kazuhiro Kikuchi(2), Mokhamad Fahrudin(3)

(*) Corresponding Author


To elucidate the effect of energy requirement during the early embryonic development on their developmental
ability to develop to blastocyst stage, in vitro fertilized (IVF) porcine one-cell embryos were cultured in modified
North Carolina State University (NCSU)-37 supplemented with different energy substrate. Result indicated that the
cleavage rate of embryos in Pyr-Lac and Gluc-Pyr-Lact groups was significantly higher than in those in Gluc group
and Gluc-Rib group (P < 0.05). At Day 6 of culture, the highest proportion of embryos develop to the blastocyst stage
was obtained in the presence of pyruvate-lactate only. In the medium with glucose, the addition of pyruvate-lactate
or ribose slightly increased the proportion of embryos develop to the blastocyst stage, however the value were not
significantly different form those obtained in the presence of glucose only. The mean cell number in blastocysts
derived from Pyr-Lac and Gluc-Pyr-Lact groups were significantly higher than those in the Gluc group (P < 0.05).
These results indicated that the presence of glucose only, as energy substrate, during the first 2 days of in vitro culture
(IVC) caused a decrease in development of in vitro produced (IVP) porcine embryos to the blastocyst stage and mean
cell number in blastocysts .
Keywords: porcine embryos-energy substrate-in vitro culture

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