Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10

https://doi.org/10.22146/ijbiotech.7559

Nur Arfa Yanti(1*), Langkah Sembiring(2), Sebastian Margino(3)

(1) Study Program of Biology, FKIP, Haluoleo University, Kendari, IndonesiaLaboratory of Microbiology, Faculty of Biology Gadjah Mada University, Yogyakarta, Indonesia
(2) Laboratory of Microbiology, Faculty of Biology Gadjah Mada University, Yogyakarta, Indonesia
(3) Laboratory of Agriculture Microbiology, Faculty of Agriculture, Gadjah Mada University, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


A new bacterial strain that produces amylase and poly-α-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics and 16S rDNA gene sequences. Amylase activity was determined spectrophotometrically on the basis of substrate concentration reduction. PHB production was quantified with UV spectrophotometer. The polymer produced by B. megaterium PSA10 was identified by Fourier Transform Infrared spectroscopy (FTIR). The result of the study showed that the amylase specific activity B. megaterium PSA10 was 593,61 DUN/mg protein and PHB production from sago starch was 52,28 % of cell dry weight (CDW). FTIR analysis of the polymer indicated that the strain B.megaterium PSA10 was a potent PHB producer.

Keywords


Amylase, Poly--hydroxybutyrate (PHB), Bacillus megaterium, 16S rDNA

Full Text:

PDF


References

Anderson, A.J. and Dawes, E.A. 1990. Occurrence, Metabolism, Metabolic Role and Industrial Uses of Bacterial Polyhydroxyalkanoate. Microbiol. Revi. 54 (4),450-472.

Aslim, B., Yuksekdag, Z.N. and Beyatli, Y. 2002. Determination of PHB Growth Quantities of Certain Bacillus Species Isolated from Soil. Turkish Elect. Jour. Biotechn. 24-30.

Byrom, D. 1987. Polymer Synthesis by Microorganisms : Technology and Economics. Tibtech.5,246 – 250.

Espino, T.M. and Tambalo, R.D. 1997. Isolation,       Screening     and Characterization of High Yielding a- Amylase Producing Bacteria. Ann. Rep. IC Biotech. 20, 744-754.

Felsenstein, J. 1995. PHYLIP (Phylogeny Inference Package) version 3,57c. Seattle. University of Washington.

Halami, P.M. 2008. Production of polyhydroxyalkanoate from starch by the native isolate Bacillus cereus CFR06. Wor. Jour. Microbiol. and Biotech. 24, 805-812.

Holt, J.G., Krieg, N.R., Sneath, P.H.A., Stanley, J.T. and Williams, S.T. 1994. Bergey’s Manual of Determinative Bacteriology. 9th ed. Lipincot, Williams and Wilkins, Baltimore.

Kim, B.S and Chang, N.H. 1998. Production of Poly (3-hydroxybutyrate) from Starch by Azotobacter chroococcum. Biotech. Lett. 20 (2), 109-112.

Misra, A.K., Thakur, M.S., Srinivas, P. and Karanth, N.G. 2000. Screening of poly-a-hydroxybutyrate producing microorganisms using Fourier transform infrared spectroscopy. Biotech. Lett. 22,1217-1219

Ramsay, B.A., Lomaliza, K., Chavarie, C., Dube, B., Bataille, P. and Ramsay, J.A. 1990.  Production   of  Poly-(ß- H y d r o x y b u t y r i c - C o - ß - Hydroxyvaleric) Acids. App. Envir. Microbiol. 56 (7),2093-2098.

Saitou, N. and Nei, M. 1987. The Neighbour-joining Method : A New Method for Reconstructing Phylogenetic Trees, Mol. Biol. and Evol. 4,406-426.

Senior, P.J., Beech, G.A., Ritchie, G.A.F. & Dawes, E.A. 1972. The Role of Oxygen Limitation in the Formation of Poly- b-hydroxybutyrate during Batch and Continuous Culture of Azotobacter beijerinckii. Biochem.Jour.128, 1193-1201.

Singhal, R.S., Kennedy, J.F., Gopalakrishnan, S. M., Kaczmarek, A., Knill, C. J. and Akmar, P.F. 2008. Industrial production, processing, and utilization of sago palm-derived products. Carbo. Poly. 72,1-20

Song, J., S. Lee, J. Kang, H. Baek and J. Suh, 2004, Phylogenetic analysis of Strep- tomyces spp. Isolated from potato scab lesions in Korea on the basis of 16S rRNA gene and 16S-23S rDNA internally transcribed spacer Se- quences. Intl.Jour. Syst. Evol. Microbiol. 54, 203-209

Valappil, S.P., Misra, S.K., Boccaccini, A.R., Keshavars, P., Bucke, C. and Roy, I. 2007a. Large-scale Production and Efficient Recovery of PHB with Desirable Material Properties, from The Newly Characterised Bacillus cerus SPV. Jour. Biotech., 127,452-460

Valappil, S.P. Boccacini, A.R. Bucke E.A. and     Roy,   E.I.    2007b. Polyhydroxyalkanoates in Gram- positive bcteria : insights from the Genera Bacillus and Streptomyces. Antonie van leeuwenhoek. 91,1-17

Yilmaz, M., Soran, H. & Beyatli, Y. 2005. Determination of Poly-a- hydroxybutyrate (PHB) production by some Bacillus  spp. World  Jour. Microbiol. Biotech. 21.565-566.



DOI: https://doi.org/10.22146/ijbiotech.7559

Article Metrics

Abstract views : 522 | views : 470

Refbacks

  • There are currently no refbacks.


Copyright (c) 2015 Indonesian Journal of Biotechnology