Genetic recombination of bovine viral diarrhea virus subgenotype -1a and -1c in persistently infected dairy cattle
https://doi.org/10.22146/ijbiotech.54111 Sri Handayani Irianingsih(1*), Bagoes Poermadjaja(2), Hastari Wuryastuti(3), Raden Wasito(4)
(1) Disease Investigation Centre Wates, Jl. Raya Yogya-Wates Km. 27, Tromol Pos 18, Wates, Yogyakarta 55602
(2) Disease Investigation Centre Wates, Jl. Raya Yogya-Wates Km. 27, Tromol Pos 18, Wates, Yogyakarta 55602
(3) Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No.2, Sleman, Yogyakarta 55281
(4) Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No.2, Sleman, Yogyakarta 55281
(*) Corresponding Author
Abstract
The bovine viral diarrhea virus (BVDV) is a major viral pathogen in cattle worldwide. In Indonesia, diversity in subgenotypes of BVDV-1 has been observed, with the highest proportion of subgenotype -1a, followed by -1c, -1b, and -1d. So far, phylogenetic analysis of BVDV-1 is based on nucleotide sequences of the 5′ UTR and partial NS5B regions. Accuracy in identifying the subgenotype and antigenic type is critical for vaccine development and effective vaccination. The aim of this study was to determine genetic recombination of BVDV through phylogenetic analysis of five different regions (5′ UTR, NPro, E2, NS3, and NS5B) of BVDV in persistently infected dairy cattle. Five isolates were sequenced using next-generation sequencing, and data were analyzed with the CLC Genomic Workbench 9.0 and MEGA-X programs. Phylogenetic analysis based on the 5′ UTR (275 nt), NPro (504 nt), E2 (1,122 nt), NS3 (2,049 nt), and NS5B (2,157 nt) regions indicated that one BVDV isolate from Banyumas, Central Java, could be classified into different subgenotypes based on the E2 region (-1c), but the same subgenotype based on the other four regions (-1a), suggesting the presence of genetic recombination of the BVDV subgenotypes -1a and -1c in persistently infected dairy cattle.
Keywords
Full Text:
PDFReferences
Bazzucchi M, Bertolotti L, Giammarioli M, Rossi E, Petrini S, Rosati S, De Mia GM. 2017. Complete genome sequence of a bovine viral diarrhea virus subgenotype 1G strain isolated in Italy. Genome Announc. 5(17):318–319. doi:10.1128/genomeA.0026317.
Becher P, Tautz N. 2011. RNA recombination in pestiviruses: cellular RNA sequences in viral genomes highlight the role of host factors for viral persistence and lethal disease. RNA Biol. 8(2):216–224. doi:10.4161/rna.8.2.14514.
Booth RE, Thomas CJ, ElAttar LM, Gunn G, Brownlie J. 2013. A phylogenetic analysis of Bovine Viral Diarrhoea Virus (BVDV) isolates from six different regions of the UK and links to animal movement data. Vet Res. 44(1):43. doi:10.1186/129797164443.
Brodersen B. 2014. Bovine viral diarrhea virus infections: manifestations of infection and recent advances in understanding pathogenesis and control. Vet Pathol. 51(2):453–464. doi:10.1177/0300985813520250.
Chernick A, van der Meer F. 2017. Evolution of Bovine viral diarrhea virus in Canada from 1997 to 2013. Virol. 509:232–238. doi:10.1016/j.virol.2017.06.024.
Deregt D, Dubovi EJ, Jolley ME, Nguyen P, Burton KM, Gilbert SA. 2005. Mapping of two antigenic do mains on the NS3 protein of the pestivirus bovine viral diarrhea virus. Vet Microbiol. 108(12):13–22. doi:10.1016/j.vetmic.2005.02.010.
Dow N, Chernick A, Orsel K, van Marle G, van der Meer F. 2015. Genetic variability of bovine viral diarrhea virus and evidence for a possible genetic bottleneck during vertical transmission in persistently infected cattle. PloS one 10(7):e0131972. doi:10.1371/journal.pone.0131972.
Firat I, Ak S, Bozkurt HH, Ak K, Turan N, Bagcigil F. 2002. Distribution of bovine viral diarrhoea virus (BVDV) in the genital system tissues of cattle. Vet Arhiv. 72(5):235–248.
Gao S, Du J, Shao J, Lang Y, Lin T, Cong G, Zhao F, Belák S, Liu L, Chang H, et al. 2014. Genome analy sis reveals a novel genetically divergent subgenotype of bovine viral diarrhea virus in China. Infect Genet Evol. 21:489. doi:10.1016/j.meegid.2013.06.010.
Giangaspero M, Harasawa R, Weber L, Belloli A. 2008. Taxonomic and epidemiological aspects of the bovine viral diarrhoea virus 2 species through the observation of the secondary structures in the 5’genomic untranslated region. Vet Ital. 44(2):319–345.
Goens SD. 2002. The evolution of bovine viral diarrhea: a review. Can Vet J. 43(12):946.
Houe H, Baker J, Maes R, Wuryastuti H, Wasito R, Ruegg P, Lloyd J. 1995. Prevalence of cattle persistently infected with bovine viral diarrhea virus in 20 dairy herds in two counties in central Michigan and comparison of prevalence of antibodypositive cattle among herds with different infection and vaccination status. J Vet Diagn Invest. 7(3):321–326. doi:10.1177/104063879500700304.
Irianingsih S, Wuryastuty H, Wasito R, Wibawa H, Rasa FT, Poermadjaja B. 2019. Genetic analysis of NS5B gene from bovine viral diarrhea virusinfected cat tle in Central and East Java, Indonesia. Vet World. 12(7):1108. doi:10.14202/vetworld.2019.1108115.
Karimy MF. 2016. Ragam genetik bovine viral diarrhea virus genotipe 1 (Singer dan CP) dan genotipe 2 (890, Ngawi, dan Ngambal) pada NPro Region. Master’s thesis, Universitas Gadjah Mada, Yogyakarta.
LiebnerTenorio, EM. 2005. Pathogenesis. In: SM In: Goyal, e Ridpath Julia F, editors, Bovine viral diarrhea virus: diagnosis, management, and control. Iowa USA, John Wiley & Sons, 1st edition. p. 121– 143.
Nagai M, Hayashi M, Sugita S, Sakoda Y, Mori M, Murakami T, Ozawa T, Yamada N, Akashi H. 2004. Phylogenetic analysis of bovine viral diarrhea viruses using five different genetic regions. Virus Res. 99(2):103–113. doi:10.1016/j.virusres.2003.10.006.
Nagai M, Sakoda Y, Mori M, Hayashi M, Kida H, Akashi H. 2003. Insertion of cellular sequence and RNA re combination in the structural protein coding region of cytopathogenic bovine viral diarrhoea virus. J Gen Virol. 84(2):447–452. doi:10.1099/vir.0.187730.
Neill JD. 2013. Molecular biology of bovine viral diarrhea virus. Biol. 41(1):2–7. doi:10.1016/j.biologicals.2012.07.002.
Peterhans E, Schweizer M. 2010. Pestiviruses: how to outmaneuver your hosts. Vet Microbiol. 142(12):18– 25. doi:10.1016/j.vetmic.2009.09.038.
Reichel MP, Lanyon SR, Hill FI. 2018. Perspectives on Current Challenges and Opportunities for Bovine Viral Diarrhoea Virus Eradication in Australia and New Zealand. Pathogens 7(1):14. doi:10.3390/pathogens7010014.
Ridpath JF. 2005. Classification and Molecular Biology. In: SM In: Goyal, e Ridpath Julia F, editors, Bovine viral diarrhea virus: diagnosis, management, and control. Iowa USA, John Wiley & Sons, 1st edition. p. 65–80.
Saepulloh M, Sendow I, et al. 2015. Identification and characterization of bovine viral diarrhea virus from Indonesian cattle. J. Vet 16(1):1–7.
Vilcek S, Durkovic B, Kolesárová M, GreiserWilke I, Pa ton D. 2004. Genetic diversity of international bovine viral diarrhoea virus (BVDV) isolates: identification of a new BVDV1 genetic group. Vet Res. 35(5):609– 615. doi:10.1051/vetres:2004036.
Vilcek S, Durkovic B, Kolesarova M, Paton D. 2005. Genetic diversity of BVDV: consequences for classification and molecular epidemiology. Prev Vet Med. 72(12):31–35. doi:10.1016/j.prevetmed.2005.08.004.
Wiyono A, Ronohardjo P, Graydon R, Daniels P. 1989. Diare ganas sapi: 1. Kejadian penyakit pada sapi Bali bibit asal Sulawesi Selatan yang baru tiba di Kaliman tan Barat. Penyakit Hewan 21(38):77–83.
Wuryastuti H, Wasito R, Sugiyono. 2018. Genotypes and biotypes variation of bovine viral diarrhea virus from persistently infected dairy cattle in Java, Indonesia. Integr J Vet Biosci 3(2):1–7.
Wuryastuti H, Putro PP, Wasito R, Maes RK. 2015. Genetic Variability of Bovine Viral Diarrhea Virus in Persistently Infected Cattle in Central Java, Indonesia. Merit Res J Microbiol Biol Sci. 3(3):031–036.
Yeşilbağ K, Alpay G, Becher P. 2017. Variability and global distribution of subgenotypes of bovine viral diarrhea virus. Viruses 9(6):128. doi:10.3390/v9060128.
Yeşilbağ K, Förster C, Ozyiğit MO, Alpay G, Tuncer P, Thiel HJ, König M. 2014. Characterization of bovine viral diarrhoea virus (BVDV) isolates from an outbreak with haemorrhagic enteritis and severe pneumonia. Vet Microbiol. 169(12):42–49. doi:10.1016/j.vetmic.2013.12.005.
DOI: https://doi.org/10.22146/ijbiotech.54111
Article Metrics
Abstract views : 2631
| 
views : 2838
Refbacks
- There are currently no refbacks.
Copyright (c) 2020 The Author(s)
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Past issues
The Indonesian Journal of Biotechnology (print ISSN 0853-8654; online ISSN 2089-2241) is published by the Research Center for Biotechnology in collaboration with the Graduate School of Universitas Gadjah Mada. The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License, and attributable to Siti Nurleily Marliana and Joaquim Baeta. Built on the Public Knowledge Project's OJS 2.4.8.1 and designed by Joaquim Baeta. View website statistics.