Molecular characterization of ageratum enation virus and beta satellite associated with leaf curl disease of fenugreek in India

P Swarnalatha; V Venkataravanappa; C N Lakshminarayana Reddy; M Sunil Kumar; M Krishna Reddy

doi:10.22146/ijbiotech.49939

Molecular characterization of ageratum enation virus and beta satellite associated with leaf curl disease of fenugreek in India

https://doi.org/10.22146/ijbiotech.49939

P Swarnalatha⁽¹⁾, V Venkataravanappa^(2*), C N Lakshminarayana Reddy⁽³⁾, M Sunil Kumar⁽⁴⁾, M Krishna Reddy⁽⁵⁾

(1) Indian Institute of Horticultural Research (IIHR), Hessaraghatta Lake PO, Bangalore, Karnataka-560089
(2) Indian Institute of Horticultural Research (IIHR), Hessaraghatta Lake PO, Bangalore, Karnataka-560089; Central Horticultural Experiment Station (CHES), Chettalli Rd, Chetalli, Karnataka-571248
(3) Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore, Karnataka-560065
(4) Indian Institute of Horticultural Research (IIHR), Hessaraghatta Lake PO, Bangalore, Karnataka-560089
(5) Indian Institute of Horticultural Research (IIHR), Hessaraghatta Lake PO, Bangalore, Karnataka-560089
(*) Corresponding Author

Abstract

Cisplatn is one of the chemotherapy for the treatment of triple‐negatve breast cancer (TNBC), but its eﬀectveness is limited because of the phenomenon of chemoresistance. miR‐638 was shown to regulate chemoresistance; however, it has never been validated in the cisplatn‐resistant tumor from patents. This present study aimed to identfy the key gene regulatory networks of miR‐638 and evaluate the potental role of the miR‐638 and its targets as potental prognosis biomarkers for cisplatn‐resistance triple‐negatve breast cancer patents. The miR‐638 target was obtained from the miRecords database while the mRNA of chemoresistance biomarker candidate was obtained from the GSE18864 of GEO database, which is mRNA of cisplatn‐resistance TNBC patents. CCND1 and FZD7 are potental candidates for cisplatn chemoresistance biomarkers in patents with TNBC. Moreover, a Kaplan‐Meier survival plot showed that breast cancer patents with low mRNA levels of FZD7 had signifcantly worse overall survival than those in higher mRNA expression group. Taken together, miR‐638 plays a role in cisplatn resistance mechanism through a mechanism involving its target gene CCND1 and FZD7. Overall, miR‐638, CCND1, and FZD7 are candidates for cisplatn biomarker resistance in TNBC.

Keywords

ageratum enation virus; ageratum yellow leaf curl beta satellite; fenugreek; leaf curl disease

Full Text:

PDF

References

Acharya SN, Thomas JE, Prasad R, K BS. 2010. Dis eases of fenugreek (Trigonella foenumgraecum L.) and control measures with special emphasis on fun gal diseases, chapter 19, pages 245–262. Wallingford: CABI. doi:10.1079/9781845936037.0245.

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J Mol Biol 215(3):403–410. doi:10.1016/S0022 2836(05)803602.

ArgüelloAstorga GR, RuizMedrano R. 2001. An iteron related domain is associated to Motif 1 in the replication proteins of geminiviruses: identification of potential interacting amino acidbase pairs by a comparative approach. Arch Virol 146(8):1465–1485. doi:10.1007/s007050170072.

Bhaskar RBL, Summanwar AS. 1982. Physical properties and host range of methi mosaic wilt virus [fenugreek: Trigonella foenumgraecum L.]. Indian Phytopathol 35:688–689.

Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X, Fauquet CM. 2008. Recommendations for the classification and nomenclature of the DNAβ satellites of begomoviruses. Arch Virol 153(4):763– 781. doi:10.1007/s0070500700136.

Briddon RW, Bull SE, Amin I, Idris AM, Mansoor S, Bedford ID, Dhawan P, Rishi N, Siwatch SS, AbdelSalam AM et al. 2003. Diversity of DNA β, a satellite molecule associated with some monopartite begomoviruses. Virology 312(1):106–121. doi:10.1016/S00426822(03)002009.

Briddon RW, Bull SE, Mansoor S, Amin I, Markham PG. 2002. Universal primers for the PCRmediated amplification of DNA β. Mol Biotechnol 20(3):315–318. doi:10.1385/MB:20:3:315.

Brown JK, Zerbini FM, NavasCastillo J, Moriones E, RamosSobrinho R, Silva JCF, FialloOlivé E, Brid don RW, HernándezZepeda C, Idris A et al.. 2015. Revision of Begomovirus taxonomy based on pairwise sequence comparisons. Arch Virol 160(6):1593–1619. doi:10.1007/s007050152398y.

Champawat RS, Singh V. 2007. Seed Spices, pages 197–232. Jodhpur: Scientific Publishers (India).

Doyle JJ, Doyle JL. 1990. Isolation of plant DNA from fresh tissue. Focus 12(13):39–40.

Fauquet CM, Bisaro DM, Briddon RW, Brown JK, Harri son BD, Rybicki EP, Stenger DC, Stanley J. 2003. Virology division news: revision of taxonomic criteria for species demarcation in the family Geminiviridae, and an updated list of begomovirus species. Arch Virol 148(2):405–421. doi:10.1007/s0070500209575.

Fischer HU, Lockhart BEL. 1976. A Moroccan isolate of turnip mosaic virus infectious to garden pea and other legumes. Plant Dis Rep 60(5):398–401.

HanleyBowdoin L, Settlage SB, Orozco BM, Nagar S, Robertson D. 1999. Geminiviruses: models for plant DNA replication, transcription, and cell cycle regulation. Crit Rev Plant Sci 18(1):71–106. doi:10.1080/07352689991309162.

Kumar J, Gunapati S, Singh SP, Gadre R, Sharma NC, Tuli R. 2013. Molecular characterization and pathogenicity of a carrot (Daucus carota) infecting begomovirus and associated betasatellite from India. Virus Res 178(2):478–485. doi:10.1016 /j.virusres. 2013.10.010.

Kumar S, Stecher G, Tamura K. 2016. MEGA7: molec ular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874. doi:10.1093/molbev/msw054.

Kumar Y, Bhardwaj P, Hallan V, Zaidi AA. 2010. Detection and characterization of Ageratum enation virus and a nanoviruslike satellite DNA1 from zinnia caus ing leaf curl symptoms in India. J Gen Plant Pathol 76(6):395–398. doi:10.1007/s1032701002701.

Kumar Y, Hallan V, Zaidi A. 2011. First report of Ageratum enation virus infecting Crassocephalum crepidioides (Benth.) S. Moore and Ageratum conyzoides L. in India. J Gen Plant Pathol 77(3):214–216. doi:10.1007/s103270110308z.

Leppik EE. 1959. World distribution of Cercospora traversiana. FAO Plant Prot Bull 8:19–21.

Martin DP, Murrell B, Golden M, Khoosal A, Muhire B. 2015. RDP4: Detection and analysis of recombination patterns in virus genomes. Virus Evol 1(1):1–5. doi:10.1093/ve/vev003.

Muhire BM, Varsani A, Martin DP. 2014. SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PLoS One 9(9):e108277. doi:10.1371/journal.pone.0108277.

Paul S, Ghosh R, Das S, Palit P, Acharyya S, Das A, Mir J, Chaudhuri S, Ghosh S, Roy A et al. 2009. First report of Tomato leaf curl Joydebpur virus and as sociated betasatellite in kenaf (Hibiscus cannabinus) plants showing leaf curl symptoms from southern India. Plant Pathol 58(2). doi:10.1111/j.1365 3059.2008.01929.x.

Petropoulos GA. 2002. Fenugreek: The Genus Trigonella. London: CRC Press, 1st edition.

Raj SK, Snehi SK, Khan MS, Tiwari AK, Rao GP. 2010. Detection of Ageratum enation virus from cat’s whiskers (Cleome gynandra L.) with leaf curl symptoms in India. J Gen Plant Pathol 76(4):292–294. doi:10.1007/s1032701002434.

Raj SK, Snehi SK, Khan MS, Tiwari AK, Rao GP. 2011. Molecular identification of an Ageratum enation virus isolate associated with mosaic disease of pointed gourd (Trichosanthes dioica) in India. Phytoparasitica 39(5):497–502. doi:10.1007/s1260001101824.

Ryley MJ. 1989. Cercospora traversiana on fenugreek (Trigonella foenumgraecum) in Queens land. Australas Plant Pathol 18(3):60–63. doi:10.1071/APP9890060.

Seal SE, Jeger MJ, Van den Bosch F. 2006a. Begomovirus evolution and disease management. Adv Virus Res 67:297–316. doi:10.1016/S00653527(06)670085.

Seal SE, Van den Bosch F, Jeger MJ. 2006b. Fac tors influencing begomovirus evolution and their in creasing global significance: implications for sustainable control. Crit Rev Plant Sci 25(1):23–46. doi:10.1080/07352680500365257.

Swarnalatha P, Kanakala S, Manasa M, Jalali S, Reddy MK. 2013. Molecular characterization of tobacco curly shoot virus infecting tomato (Solanum lycopersicum L.) in India. Pest Manag Hort Ecosyst 19(1):73–84.

Tahir M, Amin I, Haider M, Mansoor S, Briddon R. 2015. Ageratum enation virus—A Begomovirus of Weeds with the Potential to Infect Crops. Viruses 7(2):647– 665. doi:10.3390/v7020647.

Varma A, Malathi VG. 2003. Emerging geminivirus problems: a serious threat to crop production. Ann Appl Biol 142(2):145–164. doi:10.1111/j.1744 7348.2003.tb00240.x.

Venkataravanappa V, Kodandaram MH, Reddy CNL, Shankarappa KS, Reddy MK. 2017. Comparative transmission of Bhendi yellow vein mosaic virus by two cryptic species of the whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae). 3 Biotech 7(5):331. doi:10.1007/s1320501709708.

Venkataravanappa V, Reddy CNL, Jalali S, Reddy MK. 2012. Molecular characterization of distinct bipartite begomovirus infecting bhendi (Abelmoschus esculentus L.) in India. Virus Genes 44(3):522–535. doi:10.1007/s112620120732y.

Venkataravanappa V, Swarnalatha P, Reddy CNL, Chauhan N, Reddy MK. 2016. Association of recombinant Chilli leaf curl virus with enation leaf curl disease of tomato: a new host for chilli begomovirus in India. Phytoparasitica 44(2):213–223. doi:10.1007/s1260001605109.

Zimmer RC. 1984. Cercospora leaf spot and powdery mildew of fenugreek, a potential new crop in Canada. Can Plant Dis Surv 64(2):33–35.

DOI: https://doi.org/10.22146/ijbiotech.49939