NMR-metabolomics revealed metabolites and bioactivity variation in Torbangun leaves (Plectranthus amboinicus L.) of different origin

https://doi.org/10.22146/ijbiotech.38659

Nancy Dewi Yuliana(1*), Muhammad Anwari Sugiharto(2), Hanifah Nuryanie Lioe(3), Masao Goto(4), Yuko Takano Ishikawa(5)

(1) Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University, IPB Dramaga, Bogor 16680, Indonesia
(2) Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University, IPB Dramaga, Bogor 16680, Indonesia
(3) Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University, IPB Dramaga, Bogor 16680, Indonesia
(4) Functionality Evaluation Unit, Food Function Division, Food Research Institute, NARO, Tsukuba-Ibaraki, Japan
(5) Functionality Evaluation Unit, Food Function Division, Food Research Institute, NARO, Tsukuba-Ibaraki, Japan
(*) Corresponding Author

Abstract


Plectranthus amboinicus (PA) were previously reported to have antidiabetic and antioxidant activities. Environmental factors might influence the plant’s  secondary metabolite profile and their health beneficial properties. NMR-metabolomics was used to show phytochemicals variation between the Plectranthus ambonicus grown in Japan and that grown in Indonesia. The results showed that flavonoids and triterpenes were among discriminating factors of the variation between the two groups. Targeted comparative analysis of the concentration of the specific flavonoids of the plants using a validated HPLC-MWD method showed that Japanese samples contained higher concentration of total flavonoids than that of Indonesian samples. The Japanese and Indonesian samples contained 1100.6 ± 5.1 and 532.4 ± 1.8 µg/g  luteolin , 584.5 ± 7.4 and 571.7 ± 11.6 µg/g  apigenin, respectively. Eriodyctiol was detected only in Indonesian samples. Contrarily, more intensive DPPH reduction and α-glucosidase inhibition activities were found in the  Indonesian samples (IC50 14.4 ± 1.2 and 24.0 ± 0.3 µg/mL for the DPPH assay, 1181.9 ± 113.5 and 4451.4 ± 290.0 µg/mL for α-glucosidase inhibition, respectively). Thus, the flavonoids might not the only group of compounds related to the bioactivity, but it should be confirmed  by further research targetting other groups of compounds such as triterpenes. 


Keywords


Plectranthus amboinicus, metabolomics, flavonoids, antidiabetes and antoxidant



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DOI: https://doi.org/10.22146/ijbiotech.38659

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