Evaluation of potential gene expression as early markers of insulin resistance and non-alcoholic fatty liver disease in the Indonesian population


Eunice Limantara(1), Felicia Kartawidjajaputra(2*), Antonius Suwanto(3)

(1) Faculty of Biotechnology, Atma Jaya Catholic University, Jalan Jendral Sudirman 51, Jakarta Selatan 12930, Indonesia
(2) Nutrifood Research Center, PT Nutrifood Indonesia, Jalan Rawa Bali II No. 3, Jakarta Timur 13920, Indonesia
(3) Faculty of Biotechnology, Atma Jaya Catholic University, Jalan Jendral Sudirman 51, Jakarta Selatan 12930, Indonesia
(*) Corresponding Author


Early detection of insulin resistance (IR) or non-alcoholic fatty liver disease (NAFLD) is crucial to prevent future risk of developing chronic diseases. Homeostatic Model Assessment of Insulin Resistance (HOMA-IR), Liver Fat Score (LFS), and Fatty Liver Index (FLI) are generally employed to measure severity stages of IR and NAFLD. The study of gene expression could explain the molecular mechanisms that occur in the early condition of IR and NAFLD; thus providing potential early markers for both diseases. This study was conducted to evaluate the expression of genes that could potentially be an early marker of IR and NAFLD. All participants (n=21) were normolgycemic; 10 participants were categorized as healthy (without hepatosteatosis), 6 participants as having a higher risk of hepatosteatosis, and 5 participants as having hepatosteatosis. Gene expression analysis was performed using 2-ΔΔCT relative quantification method. There were significant differences on galnt2 (p<0.002) and sirt1 (p<0.010) expression between the first and the third tertile of HOMA-IR; and on ptpn1 (p<0.012) expression between the first and the second tertile of LFS. In conclusion, expression of galnt2 and sirt1 could be used as markers of the early condition of IR; while the expression of ptpn1 as early marker for NAFLD.


galnt2; insulin resistance; NAFLD; ptpn1; sirt1


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DOI: https://doi.org/10.22146/ijbiotech.36975

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