The first evaluation of glucose-6-phospate dehydrogenase defciency (G6PD) gene mutation in malaria endemic region at South Central Timor (SCT) district, Eastern Indonesia 2014–2015

https://doi.org/10.22146/ijbiotech.24194

Jontari Hutagalung(1*), Hari Kusnanto(2), S. Supargiyono(3), P. Purwono(4), Sadewa Ahmad Hamim(5), Darojatun Ida(6), Satyagraha Ari Winasti(7), Novijanti Rintis(8), Triwibowo A. Garjito(9), Mega Tyas Prihatin(10), Bai Apris(11), Bansai Immanuel(12), Kik Hao Samuel(13), Hananta Linawati(14)

(1) Research unit Tropical Medicine (Tropmed), Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Research unit Tropical Medicine (Tropmed), Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Department of Parasitology, Entomology and Tropical Disease, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
(4) Department of Parasitology, Entomology and Tropical Disease, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
(5) Department of Biochemistry, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
(6) Department of Biochemistry, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
(7) Laboratory red blood cell and enzyme disorder, Eijkman Institute for Molecular Biology, Jakarta, Indonesia
(8) Laboratory red blood cell and enzyme disorder, Eijkman Institute for Molecular Biology, Jakarta, Indonesia
(9) Institute for Vector & Reservoir Control Research & Development (IVRCR&D), National Institute of Health Research and Development, Ministry of Health, Salatiga, Cental Java, Indonesia
(10) Institute for Vector & Reservoir Control Research & Development (IVRCR&D), National Institute of Health Research and Development, Ministry of Health, Salatiga, Cental Java, Indonesia
(11) Health offce of Timor Tengah Selatan District and Malaria Laboratory, Soe, Nusa Tenggara Timur Province, Indonesia
(12) Health offce of Timor Tengah Selatan District and Malaria Laboratory, Soe, Nusa Tenggara Timur Province, Indonesia
(13) Health offce of Timor Tengah Selatan District and Malaria Laboratory, Soe, Nusa Tenggara Timur Province, Indonesia
(14) Department of Pharmacology and Pharmacy, Faculty of Medicine, University of Catholic Atmajaya, Jakarta, Indonesia
(*) Corresponding Author

Abstract


Primaquine (PQ) is a key drug in the malaria pre-elimination stage. However, PQ can trigger acute
hemolysis for people with G6PD defciency (G6PDd). In 2013, 15–25 million Indonesian people were infected with malaria, with 30,000–38,000 deaths each year mostly in eastern Indonesia with API= 15.6 %. Recently, the Ministry of Health of the Republic of Indonesia announced a plan to reach the pre-elimination stage based on WHO guidelines. This study assesses whether eastern Indonesia should proceed with the activities of malaria pre-elimination. A total 555 healthy people in fve subdistricts in eastern Indonesia were selected by systematic random samping. All data were collected using a standard questionnaire, physical examination, and laboratory tests. PCR and DNA sequencing protocols followed respective manufacture’s instructions. Statistical analysis by bivariate with α= 0.05 and 95% CI were performed using the SPSS software package. Based on the nested PCR, the result showed a malaria prevalence of 32.6% with being the dominant species (52.5%). Malaria cases were found in all study sites and not using a bed net was the moost signifcant risk factors with Exp B= 1.54 with 95% CI= 0.99–2.38. G6PDd prevalence was 16.6%, the highest G6PDd ever found in Indonesia with variant molecular dominant 10.883 T>C and one sample with a heterozygous female. Malaria pre-elimination in eastern Indonesia should be delayed. High risk patients should be tested for enzyme G6PD activities before antimalarial administration.

Keywords


G6PDd; Vanua Lava; Pre-elimination malaria; Eastern Indonesia

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References

Ainoon. I, Y., H. Yu, A. L. Amir Muhriz, N. Y. Boo, S. K. Cheong and N. H. Hamidah., 2002. Glucose-6-Phosphate Dehydrogenase (G6PD) variants in Malaysian Malays. Human Mutation in Brief 574: 2-7.


BD life sciences, (2014) BD life sciencespreanalytical systems, Product catalog. 1-Becton Drive Franklin Lakes. NJ 07417. USA: 1-4.


Beutler E., Westwood B, Melemed A, Patricia Dal Borgo, Margolis D., 1995. Three new exon 10 glucose-6-phosphate dehydrogenase mutations. Blood Cells Mol. 21: 64-67.

Beutler, E. and Vullamy, T. J., 2002. Hematologically important mutations: glucose-6-phosphate dehydrogenase. Blood Cells Mol Dis.28: 93-103.


Bioron GmbH, Smart Molecular Biology Grade. 2012. Agarose 100 gram, molecular biology: manual. Catalog. No. S-604001. Rheinhorststr.18.67071. Ludwigshafen. Germany.


Clark, T.G., Fry, A.E, Auburn, S., Campino, S., Diakite, M., Green, A., Richardson, A., Teo, Y.Y., Small, K., Wilson, J., Jallow, M., Sisay-joof, F., Pinder, M., Sabeti, P., Kwiatkowski, D.P, Rockett, K.A.
2009. Allelic heterogeneity of G6PD deficiency in West Africa and severe malaria susceptibility. Eur J Hum Genet. 17: 1080-1085.


Carlos, A.G., Priscilla, W.G., Andrew, J.T., Abdisalan, M.N., Dave, L.S., Simon, I.H., Robert, W.S., 2008. The limits and intensity of Plasmodium falciparum transmission: implications for malaria control and elimination worldwide. Plos Medicine 5(2): 2-38.


David, L.S., Justin, M.C., Christinah, C., Geoffrey, J., Peter, W.G., Roly, G., Caroline, O.B., Ramanan, L., Simon, I.H. and Andrew, J.T. 2013. A sticky situation: the unexpected stability of malaria elimination. Royal Society Publishing. 10: 2-13.


Dinas Kesehatan Propinsi Nusa Tenggara Timur. 2013. Profl Kesehatan Propinsi Nusa Tenggara Timur 2012-2013, Kupang. (no publication). Indonesia: 15-26.


Dinas Kesehatan Kabupaten Timor Tengah Selatan. 2014. Profile Kesehatan Kabupaten Timor Tengah Selatan 2012-2013. Nusa Tenggara Timur (no publication), Indonesia: 25-29.


Eijkman Institute, Kementerian Kesehatan Republik Indonesia dan Unicef. 2010. Diangnosis laboratorium malaria di Indonesia: petunjuk untuk peserta pelatihan. Eijkman Institute for molecular. Diponegoro, Jakarta: 15-27.


Elyazar, Iqbal R. F., Simon I. Hay and J. Kevin Baird., 2011a. Malaria distribution, Prevalence, drug resistance and control in Indonesia. Adv Parasitol. 74: 6-9.


Elyazar, Iqbal, R.F., Peter, W., Gething, Anand, P., Patil, Hanifah, Rogayah, Rita, K., Desak, M., Wismarini, Siti, N., Tarmizi, Kevin, B. and Simon, I.H. 2011b. malaria endemicity in Indonesia in 2010.
Plos one 6. 21315: 8-12.


Faechem R. G., Philips A. A, Targett G. A, Snow R.W. 2010. Call to action: priorities for malaria elimination. Lancet. 376: 1517-1521.


Ganczakowski, M., Town, M., Bowden, D.K., Vulliamy, T.J., Kaneko, A., Clegg, J.B., Weatherall, D.J. and Luzatto L. 1995. Multiple Glucose-6-Phosphate Dehydrogenase-Deficient Variants Correlated With Malaria Endemicity in the Vanuatu Archipelago (Southwestern Pacifc). Am.J.Hum.Genet. 56: 294-301.


Garmin International Inc, (2006) Global Positioning System (GPS) MAP Garmin 76 owner’s manual. 1200 East 151st Street, Olathe, Kansas 66062, USA: 2-4.


Gonzalo, J.D., Ari, W.S., Anup, A., Kevin, B., Germana, B., Pooja, B., Nick, C., Qin, C., Janice, C., Chi, E., Mark, F., Justin, G., Jimee, H., Marcus, L., Sarah, M., Didier, M., Francois, N., Issarang, N., Nwe, N.O., Pongwit, B., Wadchara, P., Kun, Q., Judith, R., Arantxa, R., Wichai, S., Siv, S., Lasse, V. and Lorenz, V.S. 2013. G6PD testing in support of treatment and elimination of malaria: recommendations
for evaluation of G6PD test. Malarial Journal 12: 1-12.


Hay S. I., Okiro EA, Gething P. W, Patil A. P, Tatem A. J., 2010. Estimating the global clinical burden of Plasmodium falciparum malaria in 2007. Plos Med. 7(6): 2-9.


Heidi, R., Andrew, V., George, T., Andrew, J.T., Gerard, K., Ian, R., Ivor, H., Iata, H., Sam, L. and Archie C.A.C. 2010. Baseline spatial distribution of malaria prior to an elimination programme in Vanuatu. Malaria Journal. 9(150): 2-9.


HemoCue, AB. 2013. HemoCue Hb 201, operating manual. SE-262.23. Angelholm, Sweden: 3-8.

Herdiana. H., Anis, F., Puji, B.S.A., Siti, Z., Risalia, R.A., Din, S., Hari, K., Maria, E.S., Titik, Y., Ali, I., Rahmadyani, Muhammad, Y., Rita, K., Siti, N.T., Ferdinand, J.L. and William, A.H. 2013. Progress towards malaria elimination in Sabang municipality, Aceh, Indonesia. Malaria Journal. 12(42): 3-10.


Howes, R.E., Frederic, B.P., Anand, P.P., Oscar, A.N., Peter, W.G., Mewahyu, D., Mariana, M.H., Katherine, E.B., Carmencita, D.P., Kevin, B.J. and Simon, I.H. 2012. G6PD deficiency prevalence and estimates of affected populations in malaria endemic countries: a geostatistical model-based map. PLOS Medicine 9(11): 1-15.


Jansen, G., Koenderman, L., Rijsen, G., Cats, B.P., Staal, G.E. 1985. Characteristics of hexokinase, pyruvate kinase, and glucose-6-phosphate dehydrogenase during adult and neonatal reticulocyte
maturation. Am J Hematol. 20: 203-215.


KAPA Biosystems, (2013) Technical data sheet: KAPA HiFi PCR Kit. Boston, USA. 9: 1-4.


Kathy, L.B., Germana, B., Francois, N. 2010. The reality of using primaquine. Malaria Journal 9(376): 2-5.


Kementrian Kesehatan Republik Indonesia, (2010a) Penatalaksanaan kasus malaria di Indonesia. Direktorat Jenderal Pengendalian Penyakit dan Penyehatan Lingkungan (P2M&PL), Percetakan
Negara, Jakarta (no publication), Indonesia.


Kementrian Kesehatan Republik Indonesia. 2013a. Epidemiologi Malaria di Indonesia. Dirjen P2PM&PL, Jakarta (no publication), Indonesia: 2-15.


Kementerian Kesehatan Republik Indonesia. 2013b. Malaria di Indonesia. Direktur Jendral Program Pemberantasan Penyakit Menular dan Penyehatan Lingkungan (P2M&PL), Percetakan Negara, Jakarta
(no publication), Indonesia: 5-10.


Kyoung, G.Y., Ji, S.Y., Shin, H.I., Moon, J.H., Cho, S.H., Lee, W.J., Kim, J.Y. 2014. First evaluation of glucose-6-phosphate (G6PD) deficiency in vivax malaria endemic regions in the Republic of Korea.
Plos ONE 9(5): 1-10.


Lemeshow, S., Hoswer, J.R., Klar, D.W.J., Wanga, S.K. 1997. Samples size in (terjemahan) Besar Sampel dalam Penelitian Kesehatan. Yogyakarta, Indonesia: Gajah Mada University Press: 86-75.


Lorenz, V.S., Sarah, A., Fe Espino, Dennis, S., Qin, C., James, M., Kevin, B., Catherine, M., Rosalind, H., Didier, M., Germana, B., Ari, W.S., Lasse, S.V., Justine, G., Gonzalo, D., Shunmay, Y. and Ric, P. 2013. Review of knowledge gabs in glucose-6-phosphate dehydrogenase defciency detection with
regards to the safe clinical deployment of 8-aminoquinoline treatment regimens: a workshop report. Malaria Journal 12(112): 2-12.


Luzzatto, L., Metha, A., Vulliamy, T., 2001. Glucose-6-phosphate dehydrogenase defciency. In the Metabolic & Molecular Bases of Inherrited Disease. 3: 4517-4553.


Macrogen. 2014. Standard sequencing ABI applied biosystem. 10-F. 254. Beotkkot-ro Geumcheon-gu, Seoul, Republic of Korea.


Maharaj, R., Natashia, M., Ishen, S., Philip, K., Devanand, M., Aaron, M., Eric, R. and Jaishree, R. 2012. The feasibility of malaria elimination in South Africa. Malaria Journal 11(423): 1-10.


Marina, C., Giuliana, G., Estella, O., Franco, T., Franca, M., Daniela, U., Gino, S., Lucio, L. and Paolo, A. 1998. Early Phagocytosis of Glucose-6-Phosphate Dehydrogenase (G6PDd) Erythrocytes Parasitized by
Plasmodium falciparum May Explain Malaria Protection in G6PD defciency. Blood. 92.7: 2527-2534.


Marla, K.J., Tamara, D.C., Denise, N.M., Philip, J.R. and Sunil, P. 2009. Impact of the method of G6PD defciency assessment on genetic association studies of malaria susceptibility. Plos One. 4(9): 1-6.


Martin, B. 2000. An introduction to medical statistict (3rd edition). Great Clarendon street Oxford University Press, New York, USA: 185-192.

Matsuoka, H., Meiji, A., Shigeto, Y., Indah, S.T., Suhintam, P., Henyo, K. and Fumihiko, K. 2013. Five Different Glucose-6-Phophate Dehydrogenase (G6PD) Variants Found Among 11 G6PD Deficient Persons in Flores Island, Indonesia. J. Hum Genet. 48: 541–544.


Mehdi, N., Noel, S.R., Andrew, C., Caterina, P.M., Sohail, R.R., Lori, L.J., Gregory, J.K., Mark, G., Oswaldo, L.C., Josef, T.P. and Victor, R.G.2010. Association of G6PD 292A, 376G with lower haemoglobin concentration but not increased haemolysis in patients with sickle cell anaemiaNational
Institute of Health (NIH) Public Access. Br. J. Haematol. 150(2): 218-225.


Minucci, A., Kamran, M., Hwang, M.J., Cecilia, Z., Bruno, G., Ettore, C. 2012. Glucose-6-phoaphate dehydrogenase (G6PD) mutations database: Review of the “old” and update of the new mutations. Blood Cells , Molecules and Diseases, Elseviers 48: 154-165.


New England BioLabs Inc, (2013) G6PD deficiency variant enzyme. United Kingdom (UK). Nguyen, M.H., Hideaki, E., Toshihiro, M., Takahiro, T., Francis, W.H., Ilomo, H., Nobuyuki, T. and Takatoshi, K. 2008.
Glucose-6-Phosphate Dehydrogenase (G6PD) variant in East Sepik Province of Papua New Guinea: G6PD Jammu, G6PD Vanua Lava and a novel variant (G6PD Dagua). Tropical Medicine and Health 36(4):
163-169.


Nguyen, T.H., Jean, P.C., Tran, T.H.C., Nick, D., Jeremy, J. F., Tran, T.H. and Sarah, J.D. 2009. Glucose-6 Phosphate Dehydrogenase (G6PD) Mutations And Haemoglobinuria Syndrome In The Vietnamese Population. Malaria Journal. 8: 152-159.


Nick, C., Allan, P., Stephan, D. and John, N.W. 2011. Frequency of glucose-6- phosphate dehydrogenase deficiency in malaria patients from six African countries enrolled in two randomized anti-malarial clinical trial. Malaria Journal. 10, 241: 2-9.


Nicole, L., Maria, K., Marjorie, M., Brandon, T.L., Pooja, B., Sarah, M., Michael, K., Hao, Z., Huiqiang, H., Hui, J. and Gonzalo, J.D. 2014. Comparison of Quantitative and Qualitative Tests for Glucose-6-Phosphate Dehydrogenase Defciency. American Society of Tropical Medicine and Hygiene. 91(4): 854-861.


Noor, A.M., Alegana, V.A., Patil, A.P. and Snow, R.W. 2010. Predicting the unmet need for biologically targeted coverage of insecticide-treated nets in Kenya. Am. J. Trop. Med. Hyg. . 83: 854–860.

Omumbo, J.A., Hay, S.I., Snow, R.W., Tatem, A.J. and Rogers, D.J. 2005. Modelling malaria risk in East Africa at high-spatial resolution. Trop. Med. Int. Health. 10: 557-566.


Promega corporation .2014. Wizard ® g e n o m i c D N A p u r i f i c a t i o n K i t : instruction manual. Catalog No: A-1120. 2800 Woods Hollow Road. Madison. WI-53711-5399, USA: 1-3.


Promega corporation. 2015. Ethidium Bromide Solution. Catalog No. H-5041. 2800 Woods Hollow Road. Madison, WI-53711-5399, USA.


Rajendra, M., Natashia, M., Ishen, S., Philip, K., Devanand, M., Aaron, M., Eric, R. and Jaishree, R. 2012. The feasibility of malaria elimination in South Africa. Malaria Journal. 11. 423: 2-7.


Randox Laboratories Limited. 2013. Glucose-6-Phosphate Dehydrogenase Quantitative Test Manual. Ardmore, United Kingdom.Revision. 17: 1-4.


Ranjit, K. 1999. Research methodology. SAGE publication, India. Pvt. Ltd. 32. M. Block Market, Greater Kailash-1, New Delhi, 110048: 236-240.


Satyagraha, A.W., Arkasha, S., Venessa, B., Rosalie, E., Chase, R., Iqbal, E., Rintis, N., Farah, N.C., Alida, R.H. and Kevin, B.J. 2015. G6PD defciency at Sumba in Eastern Indonesia is prevalent, diverse
and severe; implications for primaquine therapy against relapsing vivax malaria. 10. 1371: 1-17.

Smith, D.L., Smith, T.A. and Hay, S.I. 2009. Measuring malaria for elimination. Chapter 7 Shrinking the malaria map: a prospectus on malaria elimination. Santa Cruz (California): Malaria Elimination Group, Universitas of California Santa Cruz: 108–126.


Snounou, G., Viriyakosol, S., Jarra, W., Thaithong, S. and Brown, K.N. 1993. Identifcation of the four human malaria parasite species in feld samples by the polymerase chain reaction and detection of a high prevalence of mixed infections. Mol Biochem Parasitol. 58: 283-292.


Supargiyono., Micheal, T.B., Mahardika, A.W., Inge, S., Dian, N., Royhan, R., Ayleen, A.K., Sulistyawati, William, A.H., Neil, F.L., Jackie, C. and Christ, J. D. 2013. Seasonal changes in the antibody responses against Plasmodium falciparum merozoite surface antigens in areas of differing malaria endemicity in Indonesia. Malaria Journal. 12. 444: 2-9.


Syafrudin, D., Krisin, Puji, A., Sekartuti, Rita, M.D., Farah, C., Ismail, E.R., Augustina, I.S., Elyazar, Iqbal, R.F., Awalludin, S., Agus, R., Michel, K. and William, O.R. 2009. Seasonal prevalence of malaria in West Sumba District, Indonesia. Malaria Journal. 8: 1-8.


Vivantis technologies Sdn, Bhd. 2012. DNA ladder and maker VC 100 bp. Selangor, Malaysia: 2-5.


White, N. J., Qiao, L.G., Qi, G. and Luzzatto, L. 2012. Rationale for recommending a lower dose of primaquine as Plasmodium falciparum a gametocytocide in populations where G6PD defciency is common. Malaria Journal. 11: 418-425.


World Health Organization (WHO). 2014. From malaria control to malaria elimination. WHO press. Geneva: 7-35.


World Health Organization (WHO).2 0 1 4 . ” W H O G l o b a l M a l a r i a Programme: World Malaria Report 2014. 20 Avenue mber 2015Appla, 1211 Geneva, Switzerland, : 24-35.


World Health Organization (WHO). 2007. Malaria Elimination: A feld manual for low and moderate endemic countries. 20 Avenue Appla, 1211 Geneva, Switzerland, : 24-35.


Goo, Y.K., Ji, S.Y., Shin,H.I., Moon, J.H., Cho, S.H., Lee, W.J. and Kim, J.Y. 2014. First evaluation of Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency Vivax in malaria endemic regions in the Republic of Korea. Plos One 9(5): 2-6.



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