Regression Analysis for the Identification of RAPD Markers Linked To Drought Tolerance in Sorghum
https://doi.org/10.22146/ijbiotech.15997 Paramita Cahyaningrum(1), T. Taryono(2*), Anto Rimbawanto(3)
(1) Faculty of Science and Mathematics, Universitas Negeri Yogyakarta, Yogyakarta, Indonesia
(2) Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Center for Forest Biotechnology Research, Ministry of Forestry, Yogyakarta, Indonesia
(*) Corresponding Author
Abstract
Sorghum (Sorghum bicolor) can actually withstand in dry or drought condition better than other crops,
therefore it can be grown at different agroclimatic conditions and its product can be used for different purposes
such as food, feed and industrial raw material. However at severe condition, the productivity will also drop
drastically. The aim of this research was to identify RAPD marker linked to the drought tolerance. In this
research, varieties of sorghum used as research materials were Durra, Zhengzu, the mutants of Durra and
Zhengzu (from 300 Gy gamma radiation) B-100 and Zh-30, and the F2 seeds from Zh-30 x B-100 and B-100 x
Zh-30. Drought screening was carried out using 0.3 % KI during sorghum vegetative stage. DNA extraction
was done using a modified CTAB method. PCR was carried out for RAPD analysis. PCR amplification products
were scored and analyzed using SAS program. The result showed that potassium iodide can be used for
drought screening during the vegetative stage and regression analysis using the logistic method can be used
to identify RAPD markers that is linked to drought tolerance in sorghum. The logistic analysis showed that
band A8-480 was linked to drought tolerance in sorghum.
therefore it can be grown at different agroclimatic conditions and its product can be used for different purposes
such as food, feed and industrial raw material. However at severe condition, the productivity will also drop
drastically. The aim of this research was to identify RAPD marker linked to the drought tolerance. In this
research, varieties of sorghum used as research materials were Durra, Zhengzu, the mutants of Durra and
Zhengzu (from 300 Gy gamma radiation) B-100 and Zh-30, and the F2 seeds from Zh-30 x B-100 and B-100 x
Zh-30. Drought screening was carried out using 0.3 % KI during sorghum vegetative stage. DNA extraction
was done using a modified CTAB method. PCR was carried out for RAPD analysis. PCR amplification products
were scored and analyzed using SAS program. The result showed that potassium iodide can be used for
drought screening during the vegetative stage and regression analysis using the logistic method can be used
to identify RAPD markers that is linked to drought tolerance in sorghum. The logistic analysis showed that
band A8-480 was linked to drought tolerance in sorghum.
Keywords
drought; molecular marker; logistic regression; sorghum
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PDF DOI: https://doi.org/10.22146/ijbiotech.15997
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