Effect of Explant Types and Benzyl Amino Purine Concentrations on the In Vitro Regeneration of Several Local Eggplant Cultivars


Erniwitama Erniwitama(1), Taryono Taryono(2*), Rudi Hari Murti(3)

(1) Department of Agronomy, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Center of agrotechnology innovation, Universitas Gadjah Mada, Kalitirto, Berbah, Sleman, Yogyakarta 55573, Indonesia.
(3) Department of Agronomy, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author


Eggplant as a vegetable is generally fresh consumed. Besides having a high nutritive value, eggplant contains solasonine and solamargine, which act as an antibacterial that can inhibit cancer cells, gastrictic and respiratory disorders. Eggplant also provides a unique system to study in vitro regeneration since it can be induced from different explants by different growth regulators and morphogenetic pathways. This study aims to observe the effect of explant types and BAP concentrations on the in vitro regeneration of three eggplant cultivars, Rimbang,  Tanteloh and Limao. Leaf and hypocotyl explants were cultured on MS semi solid medium supplemented with BAP and IBA in combination. BAP tested at 1, 2 and 3 mgL-1 while IBA tested at 1 mgL-1. Medium containing 1 mgL-1 IBA used for rooting of regenerated shoot. The experiment was arranged in completely randomized design with five replicates. Each replicate consisted of five leaves and hypocotyl explants. Cultures were incubated at 25±10C, 65% humidity, and 16 hours per day photoperiod. Observation was done for 8 weeks of culture. The result indicated that leaf explants showed shoot initiation within 5 – 9 days of cultured, while hypocotyl explants showed the response within 7 – 12 days of cultured. Shoots formation preceded by swollen on the explants surface followed by emerging light green shoot. Leaf explants cultured on medium supplemented with 3 mgL-1 BAP + 1 mgL-1 IBA showed the maximum number of shoots regeneration while hypocotyl explants showed less of shoots  in all medium. Similarly, leaf explants cultured on medium supplemented with 3 mgL-1 BAP + 1 mgL-1 IBA showed the maximum number of roots. Among the cultivars, Rimbang showed the best response than other cultivars.


eggplant cultivars; growth regulator; hypocotyl; in vitro regeneration; leaf

Full Text:



Alicchio, R., D. Grosso, E. Boschueri. 1982. Tissue cultures and plant regeneration from different explants in six cultivars of Solanum melongena. Experientia 38: 449-450.

Arias, I. C. 2009. Selection of new eggplant (Solanum melongena, L.) lines. Dissertation. Humboldt-Universität zu Berlin. 132p.

Batti, K.H., M.D. Jamil, M. Tufail. 2014. Direct organogenesis of eggplant (Solanum melongena L.) through tissue culture. World Appl. Sci. Journal 30: 317 – 321.

Chakravarthi, D., V. Indukuri, U.A. Goparaju, V. Yechuri. 2010. Effect of genotype, explant and hormonal concentration on in vitro response of eggplant. Not.Sci Biol. 2(3):77-85.

Fassuliotis, G., B. V. Nelson, D.P. Bhat. 1981. Organogenesis in tissue culture of Solanum sisymbrifolium. J. Am. Soc. Hortic. Sci. 100: 636 - 638.

Franklin, G., C.J. Sheeba, G. Lakshmisita. 2004. Regenerated of eggplant (Solanum melongena L.) from root explants. In Vitro Cell Dev. Biol. Plant 40: 188 – 191.

Gisbert, C., J. Prothens, F. Nuez. 2006. Efficient regeneration in two potential new crops for subtropical climates, the scarlet (Solanum aethiopicum) and Gboma (Solanum macrocarpon) eggplants. New Zealand J. Crop Hortic Sci. 34: 55 – 62.

Gleddie, S., W.A. Keller, G. Setterfeld. 1983. Somatic embryogenesis and plant regeneration from leaf explants and suspension of Solanum melongena (eggplant). Can J. Botany 61: 656 – 666.

Hartman, H. T., D. E. Kester, F. T. Davis-Jr. 1990. Plant Propagation: Principles and Practices. Englewood Clifts. New Jersey: Prentice-Hall International, Inc.

Huda, A.K.M.N., M.A. Bari, M. Rahman, N. Nahar. 2007. Somatic embryogenesis of two varieties of eggplant (Solanum melongena L.). Res. J. Botany 2: 195 – 201.

Husni, A., G.A. Wattimena, I. Mariska, A. Purwito. 2003. Keragaman genetik tanaman terung hasil regenerasi protoplas. J. Biotek. Pert. 8(2):52-59.

Jagatheeswari, D., T. Ramesh. 2014. In vitro multiplication of brinjal (Solanum melongena L). Int. J. Res. Instinct 1: 107 – 120.

Kamat, M.G., N.A. Rao. 1978. Vegetative multiplication of eggplant (Solanum melongena L) using tissue culture. Plant Sci. Letter 13: 57 – 65.

Kanna, S. V., N. Jayabalan. 2010. Influence of N6-(2-Isopentenyl) Adenine on in Vitro Shoot Proliferation in Solanum melongena L. Int. J. Ac. Res. 2(2):98-100.

Kaur, M., A.S. Dhatt, J.S. Sandhu, A.S. Sidhu, S. S. Gosal. 2013. Effect of media composition and explant type on the regeneration of eggplant (Solanum melongena L.). Afr. J. Biot. 12(8):860-866.

Magioli, C., A.P.M. Rocha, D.E. De Oliveira, E. Mansur. 1998. Efficient shoot organogenesis of eggplant (Solanum melongena L.) induced by thidiazuron. Plant Cell Report 17: 661 – 663.

Magioli, C., E. Mansur. 2005. Eggplant (Solanum melongena L.): tissue culture, genetic transformation and use as an alternative model plant. Acta bot. bras. 19(1):139-148.

Mallaya, N.P., G.A. Ravishankar. 2013. In vitro propagation and genetic fidelity study of plant regenerated from inverted hypocotyls explants of eggplant (Solanum melongena L.) c.v. Arka Shirish. Biotechnology 3: 45 – 52.

Muir, K.A., A.S. Bhatt, J.S. Sandu, A.S. Sidu. 2011. Effect of genotype, explants and culture medium on organogenesis in brinjal. Indian J. Horticulture 68: 332 – 335.

Mukherjee, S.K., B. Rathinasabapathi, N. Gupta. 1991. Low sugar and osmotic requirements for shoot regeneration from leaf pieces of Solanum melongena L. Plant Cell Tissue Org. Culture 25: 13 – 18.

Murashige, T., F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiol 15: 473 – 497.

Pal, J.K., M. Singh. 2012. Effect of genotype on shoot regeneration from hypocotyl, cotyledon and leaf explants from six cultivars of eggplants (Solanum melongena L.). Int. J. Cur. Research 4: 66 – 71.

Pierik, R. L. M. 1997. Plant Tissue Culture as Motivation for the Symposium. In Van Bragt et al. [eds.]. Effects of Sterilisation on Components in Nutrient Media. Wageningen: Vennman and Zonen.

Rao, P.V.L. 1992. Difference in somatic embryogenetic ability of cultured leaf explants of four genotypes of Solanum melongena. Agronomia 12: 469 – 475.

Sarker, R. H., S. Yesmin, M.I. Haque. 2006. Multiple shoot formation in eggplant (Solanum melongena L.). Plant Tissue Culture and Biotechnology 16: 53 – 61.

Sharma, P., M.V. Rajam. 1995. Genotype, explant and position effects on organogenesis and embryogenesis in eggplant (Solanum melongena). J. Exp. Bot. 46:135-141.

Sharmin, S.A., A.H. Kabir, A. Mandal, K.K. Sharker, M.F. Alam. 2008. In Vitro propagation of eggplant through meristem culture. Agric. conspec. Sci. 73(3):149-155.

Sidhu, M.K., A.S. Dhatt, G.S. Sidhu. 2014. Plant regeneration in eggplant (Solanum melongena L.): A Review. African J. Biotech 13: 714 – 722.

Tanaka, H., M. Johkan, K. Mitsukuri, T. Tesuka, H. Furukara, M. Oda. 2012. 2012. Intact roots promote shoot regeneration from hypocotyls independent of exogenous plant growth regulators in eggplant in vitro. Plant Root 7: 5 – 11.

Wei, G., J. Wang, Y. Du. 2011. Total synthesis of solamargine. Bioorganic & Med. Chem. Let. 21:2930–2933.

Zayova, E., R. Vassillevska-Wanova, B. Kraptchev, D. Stoeva. 2012. Indirect shoot organogenesis of eggplant (Solanum melongena L.). J. Central European Agriculture 13: 446 – 457.

DOI: https://doi.org/10.22146/agrinova.41750

Article Metrics

Abstract views : 8807 | views : 1327


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.