Efect of pH on Growth, Spore Production and Spore Viability of Biocontrol Agent Trichoderma Harzianum in Submerger Fermentation


Syahiddin Dahlanr Said(1*), Masitah Hasan(2), K.B Romachandran(3)

(1) Department of Chemical Engineering, University of Malaya
(2) Department of Chemical Engineering, University of Malaya
(3) Department of Chemical Engineering, University of Malaya
(*) Corresponding Author


The effect of pH medium on growth, spore production and spore viability of bicontrol agent Trichoderma harzianum were investigated in a batch farmenter in the range of 4 to 7. The biomass concentration of this fungus at pH 4,6 and uncontrolled pH were higher than at pH 5 and 7 after 18h incubation. The spore produced by the fungus at 6,7 and uncotrolled pH was higher than others. the highest level of spore production (6.67x107 spores/ml) occured at unctrolled pH, but the spore produced in the culture grown at 7 has a higher viability (52,5%) compared to those grown at other pH.

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DOI: https://doi.org/10.22146/ajche.50855

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ASEAN Journal of Chemical EngineeringĀ  (print ISSN 1655-4418; online ISSN 2655-5409) is published by Chemical Engineering Department, Faculty of Engineering, Universitas Gadjah Mada.