Berkala Ilmiah Biologi <p><strong>BIB: Berkala Ilmiah Biologi</strong><span style="font-weight: 400;"> merupakan jurnal yang dikelola oleh Fakultas Biologi UGM. BIB menerbitkan artikel hasil penelitian dan review artikel di bidang Biologi Fungsional, Struktur dan Perkembangan, Bioteknologi dan Biologi Molekuler, serta Biologi Lingkungan dan Biodiversitas. Jurnal BIB pertama kali terbit (Vol.1 No. 1) pada tahun 1991 dalam bentuk buku hingga Volume 12 No. 1 pada tahun 2013. BIB terbit kembali secara <em>online</em> mulai Volume 13 No 1 pada bulan April 2022. Jurnal ini terbit tiga kali dalam setahun pada bulan April, Agustus, dan Desember dengan nomor ISSN 0853-7240. Paper dapat disubmit secara online dengan melakukan registrasi terlebih dahulu melalui website ini atau dapat menghubungi kami via email<br></span></p> Fakultas Biologi Universitas Gadjah Mada en-US Berkala Ilmiah Biologi 0853-7240 Reproductive Toxicity Study of Hairy Fig (Ficus hispida L.f.) Fruits on Male Wistar Rats (Rattus norvegicus Berkenhout, 1769) <p>Previous studies revealed that hairy fig fruits (HFF) are safe to consume or no-observed-adverse-effect level (NOAEL). No animals died following oral administration of unripe fruit (HFUFF) or ripe fruit (HFRFF) filtrate, as well as no signs of toxicity leading to sublethal effects. However, the effect of HFF on reproductive physiology has not been conducted. This research aimed to investigate toxic effects of HFUFF or HFRFF on male reproductive system of Wistar rats, specifically spermatogenesis, spermatozoa, and relative weight of testis and accessory sex glands. Procedure referred to OECD TG 421 with some modifications based on results of previous studies. Nine rats were assigned into three groups: HFUFF, HFRFF, and control/placebo. Each filtrate or distilled water was given orally 2 mL/individual/day for 77 days. Data were statistically analyzed based on one-way ANOVA (α=0.05). HFUFF increased values of almost all parameters. HFRFF also improved values of some other parameters, however reduced spermatogenesis index and lowered the percentage of spermatozoa with normal morphology. Animals in Control group gained the most weight and visceral fat mass. It can be concluded that HFUFF did not generate negative effects on male reproductive system of Wistar rats, in fact improved its performance. Meanwhile, consumption of HFRFF must be considered due to the finding. Both filtrates suppressed weight gain and reduced fat deposits, although unsignificant, indicating that they possess antiobesity properties. Further research related to the potential of HFF on reproductive profile and antiobesity is required.</p> Laksmindra Fitria Lisa Handayani Lina Noor Na'ilah Copyright (c) 2022 Laksmindra Fitria, Lisa Handayani, Lina Noor Na'ilah 2022-05-09 2022-05-09 13 1 1 14 10.22146/bib.v1i1.4270 Cephalometry Variation of Bataknese <p>Indonesia is a country that consists of various ethnic groups, one of the largest ethnic groups in Indonesia is Bataknese. Bataknese divided into several sub-ethnics, with the largest sub-ethnics are Batak Toba, Batak Karo and Batak Simalungun. This research aims to analyze cephalometric variation of Bataknese. An increase of various ethnic groups in various regions, followed by an increase in traffic activity and the quantity of crime can lead to an increase of traffic accidents and crime victims. Therefore, this research needs to be carried out to determine cephalometric variations and cephalometric index in the Bataknese which can be used in the process of revealing the identity of crime victims and traffic accidents. This research involved 60 subjects of Bataknese, consisting of 20 people of Batak Toba, 20 people of Batak Karo, and 20 people of Batak Simalungun. The method in this research consisted of submit ethical clearance, subject data collection, subject measurement, cephalometric index calculation, and data analysis. The results showed that Batak Toba, Karo, and Simalungun have <em>brachycephalic</em> head type, wide forehead type, and <em>platyrrhine</em> nose type. Batak Toba and Batak Karo are known to have a <em>leptoprosopic</em> facial type, while Batak Simalungun has <em>mesoprosopic</em> facial type. In this research, it was also showed that between Batak Toba and Batak Karo there were no significant differences in all body size and cephalometric data, but between Batak Toba and Batak Simalungun there were significant differences in minimum frontal width, nose width, and frontoparietal index, while between Batak Simalungun and Batak Karo there were significant differences in minimum frontal width and facial width.</p> Maria Natalina Lumban Gaol Niken Satuti Nur Handayani Copyright (c) 2022 Maria Natalina Lumban Gaol 2022-05-09 2022-05-09 13 1 15 23 10.22146/bib.v13i1.4100 Ethnobotanical Study of Tamarind (Tamarindus indica L.) in Lebakrejo Village, Purwodadi District, Pasuruan Regency <p>Tamarind (<em>Tamarindus indica</em> L.) is a plant that has many benefits ranging from leaves, stems, fruit to seeds. This study aims to evaluate the public's perception of aspects of the utilization and alanyze the distribution of tamarind in Lebakrejo Village, Purwodadi District, Pasuruan Regency. This research uses descriptive exploratory methods include: literature study, observations, interviews using questionnaires, and data analysis. The results of this study indicate that the perception of the people of Lebakrejo Village, Purwodadi District, Pasuruan Regency using tamarind in various daily needs, including herbal medicine, food ingredients, fuel, tools, animal feed, medicine, sources of income, culture, cultivation, and land boundaries. The distribution of tamarind in Lebakrejo village based on land use is distributed in the houseyard, farmland, and roadside with each frequency is 100%.</p> Siti Salma Nur Fahima Ari Hayati Hasan Zayadi Copyright (c) 2022 Siti Salma Nur Fahima 2022-05-09 2022-05-09 13 1 24 33 10.22146/bib.v13i1.4073 Characteristic Phenotype and Growth of Chicken Layer Strain (Gallus gallus domesticus, Linnaeus 1758) <p><em>The aim of this research is to study the phenotypic character and growth of layered chickens. In this study, crosses between G<sub>4</sub> Layer chickens were carried out which resulted in G<sub>5</sub> Layer chickens. Morphological characters, growth weights and measuring the feed-conversion ratio (FCR) were observed. The results obtained were 10 individuals of G<sub>5</sub> Layer chickens with the proportion of male gametes (70%) and female gametes (30%). Phenotypic characters are brown fur color and a combination of brown and white. The value of Feed Conversation Ratio (FCR) is 1.67 and the average weight growth of G<sub>5</sub> Layer chickens reaches 491.2 grams at the age of 7 weeks.</em></p> Feren Putri Sholiha Galy Hardyta Budi Setiadi Daryono Copyright (c) 2022 Feren Putri Sholiha, Budi Setiadi Daryono 2022-05-09 2022-05-09 13 1 34 39 10.22146/bib.v13i1.4329 POLIMORFISME GEN MITOKONDRIA 16S IKAN BAUNG (Hemibagrus nemurus Valenciennes, 1840) DARI SUNGAI PROGO, MAGELANG, JAWA-TENGAH <p><strong>Abstract:</strong> Asian redtail catfish (<em>Hemibagrus nemurus </em>Valenciennes, 1840) is one of Indonesian native fish species with high economic potency. However research on genetic variation of Asian redtail catfish in Indonesia is very limited. Therefore, this study purposed to identify and analyze the mitochondrial gene polymorphism <em>16S</em> of Baung fish (<em>H. nemurus</em>) from the Progo River, Magelang, Central Java. The method used in this study was the PCR method with universal primers <em>16Sar</em> and <em>16Sbr</em>. Intraspecific genetic variation was conducted by combining mitochondrial gene<em> 16S</em> sequence data of five Asian redtail fish (<em>H. nemurus</em>) samples from Progo River (BGM 02-06) and five Asian redtail fish samples from GenBank. BLAST, DnaSP, DNASTAR, GeneStudio,, MEGA, Mesquite, and NETWORK programs were used to analyze the data obtained. The results showed there were no genetic differences among the Asian redtail fish from Progo River indicating no intrapopulation genetic variation of the fish species. Conversely, the intraspecific genetic divergence was between 0.20% and 2.21% with an average of 0.92%. Analysis of intraspecific genetic variations detected 5 different haplotypes and 15 variable sites with 5 parsimony informative sites. Haplotype diversity and nucleotide diversity values were 0.756 ± 0.130 and 0.00812 ± 0.00294 respectively. Analysis of phylogenetic and haplotype network revealed BGM 02-06 formed a specific haplotype that was separated from other haplotypes. Molecular markers for Asian redtail fish (<em>H.nemurus</em>) from the Progo River, Magelang, Central Java based on the 16S mitochondrial gene can use this specific haplotype.</p> Tuty Arisuryanti Ulfa Dianiputri Katon Waskita Aji Copyright (c) 2022 Tuty Arisuryanti, Ulfa Dianiputri, Katon Waskita Aji 2022-05-24 2022-05-24 13 1 10.22146/bib.v13i1.4629