West Sumatera curd effect on fecal IgA and IgG in BALB/c mice colon inflammation induced by dextran sodium sulphate
Abstract
Curd, a fermented buffalo milk product from West Sumatra, is known for its probiotic content. Probiotics are recognized for their health benefits, including potential advantages for inflammatory bowel disease (IBD). Previous studies have confirmed the probiotic status of curd. However, the effects of curd on the immunoglobulin response in colon inflammation are still not well understood. This study employs an experimental design to investigate the impact of curd on the levels of total immunoglobulins A (IgA) and G (IgG) in feces and analyze the correlation between total IgA levels in feces and IL-10 expression in serum and feces and total IgG levels in feces and TNF-α expression in serum and feces of BALB/c with colon inflammation. In the normal group, curd administration significantly increased total IgA levels in feces, indicating a potential modulation of the immune response. Conversely, in the inflammatory group, curd reduced total IgA levels, though this effect was not statistically significant. Similarly, while curd reduced total IgG levels in feces for both the normal and inflammatory groups, the reduction was not statistically significant. No significant relationships were found between total IgA levels in feces and IL-10 expression in serum and feces, nor between total IgG levels in feces and TNF-α expression in serum and feces.
References
What is inflammatory bowel disease (IBD)? [Internet]. 2023 [cited 2024 Jun 10]. Available from: https://www.cdc.gov/ibd/what-is- IBD.
GBD 2017 Inflammatory Bowel Disease Collaborators. The global, regional, and national burden of inflammatory bowel disease in 195 countries and territories, 1990–2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet Gastroenterol Hepatol 2020; 5(1):17-30.
https://doi.org/10.1016/S2468-1253(19)30333-4
Silva FA, Rodrigues BL, Ayrizono ML, Leal RF. The immunological basis of inflammatory bowel disease. Gastroenterol Res Pract 2016; 2016:2097274.
https://doi.org/10.1155/2016/2097274
Lin R, Chen H, Shu W, Sun M, Fang L, Shi Y, et al. Clinical significance of soluble immunoglobulins A and G and their coated bacteria in feces of patients with inflammatory bowel disease. J Transl Med 2018; 16(1):359.
https://doi.org/10.1186/s12967-018-1723-0
Rengarajan S, Vivio EE, Parkes M, Peterson DA, Roberson EDO, Newberry RD, et al. Dynamic immunoglobulin responses to gut bacteria during inflammatory bowel disease. Gut Microbes 2020; 11(3):405-20.
https://doi.org/10.1080/19490976.2019.1626683
Haliman CD, Alfinnia S. Mikrobiota usus, prebiotik, probiotik, dan sinbiotik pada manajemen obesitas. Media Gizi Kesmas 2021; 10(1):149-56.
https://doi.org/10.20473/mgk.v10i1.2021.149-156
Sugitha IM, Puspawati NN. Dadih susu sapi yang dibuat dalam bambu petung bali kering (Cows Milk DadihMade In Dried Petung Bamboo Bali). Media Ilmiah Teknol Pangan (Sci J Food Technol) 2018; 5(2):80-4.
Arnold M, Rajagukguk YV, Gramza-Michałowska A. Characterization of dadih: traditional fermented buffalo milk of Minangkabau. Beverages 2021; 7(3):60.
https://doi.org/10.3390/beverages7030060
Anugerah W. Apa itu dadih dan bagaimana cara membuatnya dengan mudah [Internet]. 2023 [cited 2024 Aug 30].
https://www.localstartupfest.id/faq/apa-itu-curd/
Nurtimasia WO. Pengaruh pemberian dadih terhadap kadar protein SOCS-1, TNF-alpha dan IL-10 pada feses mencit yang diinduksi dextran sodium sulfate. Jakarta: Universitas Indonesia; 2021.
Kodariah R, Armal HL, Wibowo H, Yasmon A. The effect of dadih in BALB/c mice on pro-inflammatory and anti-inflammatory cytokine productions. J Med Sci (Berkala Ilmu Kedokteran) 2019; 51(04):292-300.
https://doi.org/10.19106/MedSci005104201902
Plaza-Diaz J, Ruiz-Ojeda FJ, Gil-Campos M, Gil A. Mechanisms of action of probiotics. Adv Nutr 2019; 10(suppl_1):S49-66.
https://doi.org/10.1093/advances/nmy063
Sturm A, Maaser C, Calabrese E, Annese V, Fiorino G, Kucharzik T, et al. ECCO-ESGAR guideline for diagnostic assessment in IBD part 2: IBD scores and general principles and technical aspects. J Crohns Colitis 2019; 13(3):273-284.
https://doi.org/10.1093/ecco-jcc/jjy114
Giorgetti GM, Brandimarte G, Fabiocchi F, Ricci S, Flamini P, Sandri G, et al. Interactions between innate immunity, microbiota, and probiotics. J Immunol Res 2015; 2015:501361.
https://doi.org/10.1155/2015/501361
Brahe LK, Astrup A, Larsen LH. Can we prevent obesity-related metabolic diseases by dietary modulation of the gut microbiota? Adv Nutr 2016; 15(7):90-101.
https://doi.org/10.3945/an.115.010587
Ellyza N. The effect of dadih to intestinal microflora balance in acute diarrhea mice induced by enteropathogenic Escherichia coli. [Skripsi]. Universitas Andalas, Padang, 2024.
http://scholar.unand.ac.id/22605/6/abstrak.pdf
Eichele DD, Kharbanda KK. Dextran sodium sulfate colitis murine model: An indispensable tool for advancing our understanding of inflammatory bowel diseases pathogenesis. World J Gastroenterol 2017; 23(33):6016-29.
https://doi.org/10.3748/wjg.v23.i33.6016
Ahluwalia B, Moraes L, Magnusson MK, Öhman L. Immunopathogenesis of inflammatory bowel disease and mechanisms of biological therapies. Scand J Gastroenterol 2018; 53(4):379-89.
https://doi.org/10.1080/00365521.2018.1447597
Kiesler P, Fuss IJ, Strober W. Experimental models of inflammatory bowel diseases. Cellular Mol Gastroenterol Hepatol 2015; 1(2):154-70.
https://doi.org/10.1016/j.jcmgh.2015.01.006
Zhao M, Chu J, Feng S, Guo C, Xue B, He K, et al. Immunological mechanisms of inflammatory diseases caused by gut microbiota dysbiosis: A review. Biomed Pharmacother 2023; 164:114985.
https://10.1016/j.biopha.2023.114985
Llewellyn A, Foey A. Probiotic modulation of innate cell pathogen sensing and signaling events. Nutrients 2017 9(10):1156.
https://doi.org/10.3390/nu9101156
Ruder B, Atreya R, Becker C. Tumour necrosis factor alpha in intestinal homeostasis and gut related diseases. Int J Mol Sci 2019; 20(8).1887.
https://doi.org/10.3390/ijms20081887
Li B, Alli R, Vogel P, Geiger TL. IL-10 modulates DSS-induced colitis through a macrophage-ROS-NO axis. Mucosal Immunol 2014; 7(4):869-78.
https://doi.org/10.1038/mi.2013.103
de Araujo GV, de Lorena VMB, Montenegro SML, de Albuquerque EC, Peixoto DM, Sarinho ESC. Probiotics as an adjunct for the treatment of recurrent wheezing in infants and effects on expression of T-helper 1 and regulatory T cytokines. J Funct Foods 2017; 35:398-407.
https://doi.org/10.1016/j.jff.2017.06.012
Yahfoufi N, Mallet JF, Graham E, Matar C. Role of probiotics and prebiotics in immunomodulation. Curr Opin Food Sci 2018; 20:82-91.
https://doi.org/10.1016/j.cofs.2018.04.006
Liu M, Zhang X, Hao Y, Ding J, Shen J, Xue Z, et al. Protective effects of a novel probiotic strain, Lactococcus lactis ML2018, in colitis: in vivo and in vitro evidence. Food Funct 2019; 10(2):1132-45.
https://doi.org/10.1039/c8fo02301h
Shahrokhi M, Nagalli S. Probiotics. [Updated 2023 Jul 3]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK553134/
Bernal AM, Fernández-Brando RJ, Bruballa AC, Fiorentino GA, Pineda GE, Zotta E, et al. Differential outcome between BALB/c and C57BL/6 mice after Escherichia coli O157:H7 infection is associated with a dissimilar tolerance mechanism. Infect Immun 2021; 89(5):e00031.
