The Initial Dioxigenase Gene Squences Analysis of Marine Bacteria Strain M128

  • Muhamad Sahlan Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI –Depok, Depok, 16424, Indonesia
  • Hanif Yuliani Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI –Depok, Depok, 16424, Indonesia; Centerfor the Industrial Processed of Technology, the Agency for the Assessment and Application of Technology (BPPT), Jakarta, 10340, Indonesia
  • Heri Hermansyah Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI –Depok, Depok, 16424, Indonesia
  • Anondho Wijanarko Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI –Depok, Depok, 16424, Indonesia
Keywords: Biodegradation, Dioxygenase, Gene sequence, Analysis, PAHs

Abstract

Biodegradation of polyaromatic hydrocarbons (PAHs) are catalyzed by multicomponent enzymes from microbe. The initial dioxygenase was used as a key enzyme for attacking the aromatic ring structure of PAHs, furthermore its initial dioxygenase gene was used to select PAHs degrading bacteria. Marine bacteria M128 strain could grow on medium contained PAHs. Detection of its cellular initial deoxygenase gene was done by nahAc gene amplification. The nahAc gene commonly used as biomarkers of PAH degradation, and as a result, nahAc gene sequence analysis of marine bacteria M128 strain was similar to naphthalene dioxygenase of Pseudomonas genera with 99% homology.

References

1. Abd-Elsalam, H., Hafed, E., Hussain, A.A., (2009), Isolation and Identification
of Tree-Rings Polyaromatic Hydrocarbon (Anthracenes and Phenantrene) Degrading Bacteria,
Fig. 6: Naphthalene degradation pathways, the nah gene specify the degradation of
naphthalene to pyruvate and acetyl-CoA. Muhamad Sahlan, Hanif Yuliani, Heri Hermansyah, and Anondho Wijanarko 41 American-Eurasian Journal of Agriculture & Environment Sciences, 5(1), pp. 31-38.
2. Arun, K., Ashok, M., Rajesh, S., (2001), Crude Oil PAH Constitution, Degradation Pathway and Assiciated Bioremediation Microflora: an Overview, International Journal of Environmental Sciences, 1(7), pp. 1420- 1439.
3. Das, K., Mukherjee, A.K., (2006), Differential Utilization of Pyrene as the Sole source of Carbon by Bacillus subtilis and Pseudomonas aeruginosa strain: Role of Biosurfactants in
enhancing Bioavailability, Journal of Applied Microbiology, 102, pp. 195-
202.
4. Grimm, A.C., Harwood, C.S., (1997), Chemotaxis of Pseudomonas spp. to the Polyaromatic Hydrocarbon Napthalene, Applied and Environmental Microbiology, 63(10), pp. 4111-4115.
5. Guo, C., Dang, Z., Wong, Y., Tam, N.F., (2010), Biodegradation Ability and Dioxygenase gene of PAH-degrading Spingomonas and Mycobacterium strains Isolated from Mangrove
Sediment, International Biodeterioration & Biodegradation, 64, pp. 419-426.
6. Helmy, Q., Kardena, E., Nurachman, Z., Wisjnuprapto, (2010), Application of Biosurfactant Produced by Azotobacter vinelandii AV01 for Enhanced Oil Recovery and Biodegradation of Oil Sludge, International Journal of Civil & Environmental Engineering, 10(1), pp.
7-14.
7. Herrick, J. B., E. L. Madsen, C. A. Batt, and W. C. Ghiorse., (1993), Polymer-ase chain reaction amplification of naphthalene catabolic and 16S rRNA gene sequences from indigenous sediment bacteria, Applied Environmental Microbiology, 59, pp. 687–694.
8. Iwabuchi, T., Harayama, S., (1997), Biochemical and Genetic Characterization of 2-
Carboxybenzaldehyde Dehydrogenase, an Enzyme Involved in Phenanthrene Degradation by Nocardioides sp. Strain KP7, Journal of Bacteriology, 179(20), pp. 6488-6495.
9. Kim, S-J., Kweon, O., Jones, R.C., Freeman, J.P., Edmondson, R.D., Cerniglia, C.E., (2007), Complete and Integrated pyrene degradation pathways in Mycobacterium vanbaalenii PYR-1 based on Systems Biology, Journal of Bacteriology, 189(2), pp. 464- 472.
10. Liu, W., Luo, Y., Teng, Y., (2010), Bioremediation of Oily Sludge- contaminated Soil by Stimulating Indigenous Microbes, Environmental Geochemical Health, 32, pp. 23-29.
11. Liang, Y., Gardner, D.R., Miller, C.D., Chen, D., Anderson, A.J., Weimer, B.C., Sims, R.C., (2006), Study of Biochemical Pathways and Enzymes Involved in Pyrene Degradation by Mycobacterium sp. Strain KMS, Applied and Environmental Microbiology, 72(12), pp. 7821-7828.
12. Prágai Z., Tjalsma H., Bolhuis A., van Dijl J. M., Venema G., Bron S., (1997), The signal peptidase II (lsp) gene of Bacillus subtilis. Microbiology 143:1327–1333.
13. Prágai Z., Harwood C. R., (2000), Screening mutants affected in their response to phosphate. in Functional analysis of bacterial genes: a practical manual. eds Schumann W., Ehrlich S. D., Ogasawara N. (John Wiley & Sons, Ltd. Chichester, United Kingdom), pp 245– 249.
14. Okoh, A., Trejo-Hernandez, M. R., (2006), Remediation of petroleum hydrocarbon polluted systems: Exploiting the bioremediation strategies, African Journal of Biotechnology, 5(25), pp. 2520-2525.
15. Stingley, R.L., Khan, A.A., Cerniglia, C.E., (2004), Molecular Characterization of a
Phenanthrene degradation pathway in Mycobacterium vanbaalenii PYR- 1, Biochemical and Biophysical Research Communications, 322, pp. 133-146.
16. Seo, J-S., Keum, Y-S., Li, Q.X., (2009), Bacterial Degradation of Aromatic Compounds, International Journal of Environmental Resources and Public Health, 6, pp. 278- 309.
17. Zhou, H.W., Guo, C.L., Wong, Y.S., Tam, N.F.Y., (2006), Genetic diversity of dioxygenase gene in polycyclic aromatics hydrocarbons-degrading bacteria isolated from mangrove Sediment, FEMS Microbiology Letters, 262, pp. 148-157.
Published
2013-12-31
How to Cite
Sahlan, M., Yuliani, H., Hermansyah, H., & Wijanarko, A. (2013). The Initial Dioxigenase Gene Squences Analysis of Marine Bacteria Strain M128. ASEAN Journal of Chemical Engineering, 13(1), 33-42. Retrieved from https://jurnal.ugm.ac.id/v3/AJChE/article/view/8139
Section
Articles