Production of Polyhvdroxyalkanoates from Synthetic Wastewater Using Sequencing Batch Reactors in On-Off Sequences
Keywords:
Activated sludge, aerated-unaerated SBR period, polyhydroxyalkanoates (PHAs), sequencing batch reactor (SBR), synthetic wastewater
Abstract
The biosynthesis of polyhydroxyalkanoates (PHAs) using activated sludge as cultureorganism from tapioca synthetic wastewater has been studied in a 6L sequencing batch reactor (SBR). It was found that longer aeration time caused greater chemical oxygen demand (COD) reduction but did not achieve the maximum PHA-production rate. The application of on-off sequences, however, gave an acceptable PHA- productivity rate at 0.1033 gPHA L-1 h-1 and a high storage yield of up to 0.32 gPHNg biomass, although it caused a decrease in pH and affected sludge stability. The on-off variation also produced a higher valerate copolymer content, in the range of 16.44-22.48 % for Run 1 and of 13.09-14.09 % for Run 2, compared to the customary aerated-unaerated variations. The study indicates that the use of on-off SBR sequences may be suitable for obtaining high PHA-storage yields.References
Akunna, J. c., and Jefferies, C. (2000). "Performance of family-size sequencing batch reactor and rotating biological contactor units treating sewage at various operating conditions." Water Sci. Techno!., 31, 97-104.
Andersson, A. J., and Dawes, E. A. (1990). "Occurrence, metabolism, metabolic role and industrial use of bacterial PHA," Microbiol. Rev., 54,450-72.
Banister, S. S., and Pretorius, W. A. (1998). "Optimisation of primary sludge acidogenic fermentation for biological nutrient removal," WaterSA., 24, 35-42.
Beun J. J., Direks, K., Heijnen, J. J., and van Loosdrecht, M. C. M. (2002). "Poly-b- hydroxybutyrate metabolism in dynamically fed mixed microbial cultures," Water Res., 36, 1167-80.
Brandl, H., Gross, R. A., Lenz, R. W., and Fuller, R. C. (1990). "Plastics from bacteria and for bacteria: Poly(a-hydroxyalkanoates) as natural, biocompatible, and biodegradable polymers," Adv. Biochem. Eng. Biotechno!., 41, 77-93.
Choi, J., and Lee, S. Y.(1997). "Process analysis and economic evaluation for poly(3- hydroxy butyrate) production by fermentation," Bioprocess Eng., 17, 335-42.
Dionisi, D., Majone, M., Papa, v., and Beccari, M. (2004). "Biodegradable polymerfrom organic acids by using activated sludge enriched by aerobic periodic feeding," Biotechnol. Bioeng., 85, 569-79.
Hesselmann, R. P X., Van Rammell, R ; Resnick, S. M., Hany, R., and Zehnder, A. J. B. (2000). "Anaerobic metabolism of bacteria performing enhanced biological phosphate removal," Water Res., 34, 3487-94.
Huijberts, G. N. M., and Eggink, G. (1996). "Production of poly(3-hydroxyalkano- ates) by Pseudomonas putida KT2442 in continuous cultures," Appl. Microbio!. Biotech., 46, 233-9.
Kim, B. S., Lee, S. c., Lee, S. Y., Chang, H. N., Chang, Y. K., and Woo, S. L. (1994). "Production of poly(3- hydroxybutyric Acid) by fed-batch culture of Alcaligenes eutrophus with glucose concentration control," Biotechnol. Bioeng., 43, 892-8.
Leaversuch, R. (1987). "Industry weighs the need to make polymer degradable," Mod. Plastic, 64, 52-5.
Lee, B., Pometto, A L., Fratzke, A, and Bailey, T. B. (1991). "Biodegradation of degradable plastic polyethylene by Phanerochaete and Streptomyces species," Appl. Environ. Microbiol., 57, 678-85.
Lee, S. Y. (1996). "Plastic bacteria? Progress and
prospects forpolyhydroxyalkanoate production in bacteria," Trends Biotechnol., 14, 431-8.
Lee. S. Y.et al. (1994). "Construction of plasmids, estimation of plasmid stability, and use of stable plasmid for the production of poly(3-hydroxybutyric acid) in Escherichia coli," J. Biotechnol., 32, 203-11.
Lee, S. Y, and Chang, H. N. (1994). "Effect of complex nitrogen source on the synthesis and accumulation of poly(3- hydroxybutyric acid) by recombinant Escherichia coli in flask and fed-batch cultures," J. Environ. Polymer Degrad., 2169-76.
Lemos, c., Viana, c., Saguciro, E. N., Rmas, A M., Crespo, S. G., and Reis, M. A M. (1998). "Effect of carbon source on the formation ofpolyhydroxyalkanoates by a phosphate accumulating mixed culture," Enzyme Microb. Technol., 22, 662-71.
Levantesi, c., Serafim, L. S., Crocetti, G. R, Lemos, P c., Rossetti, S., Blackall, L. L. et al. (1998). "Analysisof the microbial community structure and function of a laboratory-scale enhanced biological phosphorus-removal reactor," Environ. Microbiol., 22, 559-69.
Liu, W.T., Nakamura, K.,Matsuo, T.,and Mino, T. (1997). "Internal energy-based competition between polyphosphate and glycogen accumulating bacteria in biological phosphorus removal effect of PIC feeding ratio," Water Res., 31, 1430-8.
Salehizadeh, H., and van Loosdrecht, M. C. M. (2004). "Production of Poly- hydroxyalkanoates by mixed culture: Recent trends and biotechnological importance," Biotech. Adv., 22, 261-79.
Satoh, H., Iwamoto, Y, Mino, T., and Matsuo, T. (1998). "Activated sludge as a possible source of biodegradable plastics," Water Sci. Technol., 38, 103-9.
Satoh, H., Mino, T., and Matsuo, T. (1992). "Uptake of organic substrate and accumulation of polyhydroxyalkanoates linked with glycolysis of intracellular carbohydrates under anaerobic conditions in biological excess phosphorus-removal process," WaterSci. Technol.,26,933-42.
Satoh, H., Ramey, W.D., Koch, f A, Oldham, W. K., Mino, T., and Matsuo, T. (1996). "Anaerobic substrate uptake by the enhanced biological phosphorus- removal activated sludge," Water Sci. Technol., 34, 9-16.
Sidikmarsudi, S. A. (1997). "Kajian Awal Pembentukan PHA oleh Bakteri Fotosintetik Rhodobacter spheroides (IFO 12203) pada Medium Asam Lemak Volatil," Jurusan Teknik Kimia ITB, Bandung, Indonesia.
Smolders, G. J. F., Vander Meij, J., van Loosdrecht, M. C. M., and Heijnen, J. J. (1994). "Model of anaerobic metabolism of biological phosphorus- removal processes: Stoichiometry and pH influence," Biotechnol. Bioeng., 43, 461-70.
Steinbuchel, A, and Valentin, H. E. (1995). "Diversity of bacterial polyhydroxy- alkanoic acid," FEMS Microbiol. Letter, 128,219-28.
Yamane, T. (1993). "Yield of poly-D(-)-3- hydroxybutyrate from various carbon sources: A theoretical study," Biotechnol. Bioeng., 41, 165-70.
Yamane, T., Fukunaga, M., and Lee, Y. W. (1996). "Increased PHB productivity by high cell-density fed-batch culture of Alcaligenes latus, A growth-associated PHB producer," Biotechnol. Bioeng., 50, 197-202.
Yu, P, Chua, H., Huang, A L., Lo, W., and Tam. (1999). "Conversion of industrial food wastes by Alcaligenes latus into polyhydroxyalkanoates," Department of Applied Biology and Chemical Technology, Department of Civil and Structural Engineering, Hong Kong Polytechnic University, Hong Kong.
Andersson, A. J., and Dawes, E. A. (1990). "Occurrence, metabolism, metabolic role and industrial use of bacterial PHA," Microbiol. Rev., 54,450-72.
Banister, S. S., and Pretorius, W. A. (1998). "Optimisation of primary sludge acidogenic fermentation for biological nutrient removal," WaterSA., 24, 35-42.
Beun J. J., Direks, K., Heijnen, J. J., and van Loosdrecht, M. C. M. (2002). "Poly-b- hydroxybutyrate metabolism in dynamically fed mixed microbial cultures," Water Res., 36, 1167-80.
Brandl, H., Gross, R. A., Lenz, R. W., and Fuller, R. C. (1990). "Plastics from bacteria and for bacteria: Poly(a-hydroxyalkanoates) as natural, biocompatible, and biodegradable polymers," Adv. Biochem. Eng. Biotechno!., 41, 77-93.
Choi, J., and Lee, S. Y.(1997). "Process analysis and economic evaluation for poly(3- hydroxy butyrate) production by fermentation," Bioprocess Eng., 17, 335-42.
Dionisi, D., Majone, M., Papa, v., and Beccari, M. (2004). "Biodegradable polymerfrom organic acids by using activated sludge enriched by aerobic periodic feeding," Biotechnol. Bioeng., 85, 569-79.
Hesselmann, R. P X., Van Rammell, R ; Resnick, S. M., Hany, R., and Zehnder, A. J. B. (2000). "Anaerobic metabolism of bacteria performing enhanced biological phosphate removal," Water Res., 34, 3487-94.
Huijberts, G. N. M., and Eggink, G. (1996). "Production of poly(3-hydroxyalkano- ates) by Pseudomonas putida KT2442 in continuous cultures," Appl. Microbio!. Biotech., 46, 233-9.
Kim, B. S., Lee, S. c., Lee, S. Y., Chang, H. N., Chang, Y. K., and Woo, S. L. (1994). "Production of poly(3- hydroxybutyric Acid) by fed-batch culture of Alcaligenes eutrophus with glucose concentration control," Biotechnol. Bioeng., 43, 892-8.
Leaversuch, R. (1987). "Industry weighs the need to make polymer degradable," Mod. Plastic, 64, 52-5.
Lee, B., Pometto, A L., Fratzke, A, and Bailey, T. B. (1991). "Biodegradation of degradable plastic polyethylene by Phanerochaete and Streptomyces species," Appl. Environ. Microbiol., 57, 678-85.
Lee, S. Y. (1996). "Plastic bacteria? Progress and
prospects forpolyhydroxyalkanoate production in bacteria," Trends Biotechnol., 14, 431-8.
Lee. S. Y.et al. (1994). "Construction of plasmids, estimation of plasmid stability, and use of stable plasmid for the production of poly(3-hydroxybutyric acid) in Escherichia coli," J. Biotechnol., 32, 203-11.
Lee, S. Y, and Chang, H. N. (1994). "Effect of complex nitrogen source on the synthesis and accumulation of poly(3- hydroxybutyric acid) by recombinant Escherichia coli in flask and fed-batch cultures," J. Environ. Polymer Degrad., 2169-76.
Lemos, c., Viana, c., Saguciro, E. N., Rmas, A M., Crespo, S. G., and Reis, M. A M. (1998). "Effect of carbon source on the formation ofpolyhydroxyalkanoates by a phosphate accumulating mixed culture," Enzyme Microb. Technol., 22, 662-71.
Levantesi, c., Serafim, L. S., Crocetti, G. R, Lemos, P c., Rossetti, S., Blackall, L. L. et al. (1998). "Analysisof the microbial community structure and function of a laboratory-scale enhanced biological phosphorus-removal reactor," Environ. Microbiol., 22, 559-69.
Liu, W.T., Nakamura, K.,Matsuo, T.,and Mino, T. (1997). "Internal energy-based competition between polyphosphate and glycogen accumulating bacteria in biological phosphorus removal effect of PIC feeding ratio," Water Res., 31, 1430-8.
Salehizadeh, H., and van Loosdrecht, M. C. M. (2004). "Production of Poly- hydroxyalkanoates by mixed culture: Recent trends and biotechnological importance," Biotech. Adv., 22, 261-79.
Satoh, H., Iwamoto, Y, Mino, T., and Matsuo, T. (1998). "Activated sludge as a possible source of biodegradable plastics," Water Sci. Technol., 38, 103-9.
Satoh, H., Mino, T., and Matsuo, T. (1992). "Uptake of organic substrate and accumulation of polyhydroxyalkanoates linked with glycolysis of intracellular carbohydrates under anaerobic conditions in biological excess phosphorus-removal process," WaterSci. Technol.,26,933-42.
Satoh, H., Ramey, W.D., Koch, f A, Oldham, W. K., Mino, T., and Matsuo, T. (1996). "Anaerobic substrate uptake by the enhanced biological phosphorus- removal activated sludge," Water Sci. Technol., 34, 9-16.
Sidikmarsudi, S. A. (1997). "Kajian Awal Pembentukan PHA oleh Bakteri Fotosintetik Rhodobacter spheroides (IFO 12203) pada Medium Asam Lemak Volatil," Jurusan Teknik Kimia ITB, Bandung, Indonesia.
Smolders, G. J. F., Vander Meij, J., van Loosdrecht, M. C. M., and Heijnen, J. J. (1994). "Model of anaerobic metabolism of biological phosphorus- removal processes: Stoichiometry and pH influence," Biotechnol. Bioeng., 43, 461-70.
Steinbuchel, A, and Valentin, H. E. (1995). "Diversity of bacterial polyhydroxy- alkanoic acid," FEMS Microbiol. Letter, 128,219-28.
Yamane, T. (1993). "Yield of poly-D(-)-3- hydroxybutyrate from various carbon sources: A theoretical study," Biotechnol. Bioeng., 41, 165-70.
Yamane, T., Fukunaga, M., and Lee, Y. W. (1996). "Increased PHB productivity by high cell-density fed-batch culture of Alcaligenes latus, A growth-associated PHB producer," Biotechnol. Bioeng., 50, 197-202.
Yu, P, Chua, H., Huang, A L., Lo, W., and Tam. (1999). "Conversion of industrial food wastes by Alcaligenes latus into polyhydroxyalkanoates," Department of Applied Biology and Chemical Technology, Department of Civil and Structural Engineering, Hong Kong Polytechnic University, Hong Kong.
Published
2004-12-31
How to Cite
Setiadi, T., Sondjaja, herriyanto R., & Veeriansyah, B. (2004). Production of Polyhvdroxyalkanoates from Synthetic Wastewater Using Sequencing Batch Reactors in On-Off Sequences. ASEAN Journal of Chemical Engineering, 4(1), 11-18. Retrieved from https://jurnal.ugm.ac.id/v3/AJChE/article/view/7581
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