UJI SITOKSISITAS CAMPURAN RESIN AKRILIK DENGAN KITOSAN SEBAGAI BAHAN GIGI TIRUAN ANTI JAMUR

https://doi.org/10.22146/teknosains.27580

Titik Ismiyati(1*), Widowati Siswomihardjo(2), Marsetyawan H.N.E. Soesatyo(3), Rochmadi Rochmadi(4)

(1) Fakultas Kedokteran Gigi, Universitas Gadjah Mada
(2) Fakultas Kedokteran Gigi, Universitas Gadjah Mada
(3) Fakultas Kedokteran Umum, Universitas Gadjah Mada
(4) Fakultas Teknik, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Denture acrylic resin contain monomer residue that can cause allergic reactions and inflammation in the mouth. Chitosan has advantage biocompatible and antifungal. The purpose of this study was to examine toxicity acrylic resin blends with chitosan as denture antifungal in fibroblast cell culture. This study uses chitosan concentration 0.5%, 1%, 2% and 4% of 2.5 ml were blends with acrylic resin. Fibroblast cell cellular responses were assessed using MTT assay. Data at the cell viability analyzes used Anova one path (p <0.05). The results showed the greatest average adsorbansi fibroblast cell in blends acrylic resin was chitosan concentration of 0.5% (0.434 ± 0.119) with 99.810% cell viability, and the smallest average chitosan concentration of 4% (0.385 ± 0.023) and 88.523% cell viability. Anova test showed there were differences the effect of varying concentrations of chitosan significantly to adsorbansi and cell viability (p <0.05). The results of post hoc test showed a concentration of 4% was significantly different than other concentration. Conclusion, acrylic resin blends with chitosan at a concentration of 0.5%, 1%, 2% were non-toxic, being mild toxic concentration of 4%.


Keywords


Denture; Acrylic Resin; Chitosan; Cytotoxicity

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References

Balicka-Ramisz,A., Pajak,A.W., Pilarczyk,B., Ramisz, A., Laurans, L., 2005, Antibacterial and Antifungal Activity of chitosan, ISAH - Warsawa, Poland Vol 2 :406-408.

Bell,J.A., Brockmann,S.L, Feil,P., Sackovich, D.A., 1989, The Effectiveness of Two Disinfectans on Denture Base Acrylic Resin With An Organik load, J.Prosthet.Dent., 61 (5) : 580 – 582

Boynuegri,D., Ozcan g., Senel,S., Uc,d., Uraz,a., Ogus, E., Cakilci,B., Karaduman, B., 2009, Clinical and radiographic evaluation of Chitosan gel in Periodontal intraosseus defect : a Pilot study, J Biomed Mater Res Part B: Appl Biomater 90B (1) : 461-466

Brunner,T.J., Wick,P., Manser,P., Spohn,P., Grass, R.N., Limbach,L.K., Bruinink,A., and Stark,W.J., 2006, In Vitro Cytotoxicity of Oxide Nanoparticles : Comparation to Asbestos, Silica, and The Effect of particle solubility, Environ Sci Technol, 40 : 4374 -81

Budtz- Jorgensen,E., 2004, Sequelae caused by wearing complete dentures,dalam Zarb,G.A., Bolender,C.L., Prosthodontic treatment for edentulous patients Complete denture and implant –supported prostheses, 12th ed., Mosby Inc., St. Louis, 34-50.

Combe, E.C., 1992, Notes on Dental Materials, 6th ed., Churchill Livingstone, New York, 223- 5

Cotran, M.D., Kumar, V., dan Collins, T., 1999, Robbins pathologic of disease, 6th ed., W.B. Saunders Company, Philadelpia, h:6-8, 4-15, 102-110.

Goy, R.C., Britto,D., Assis,O.B.G., 2009 A. Review of The Antimicrobial Activity of Chitosan, Polimeros : Ciencia etecnologia , 19 (3) : 1-7

Ismiyati,T., 2012, Effectiveness of High Molecular Weight Chitosan on The Growth of Candida Albicans in Thermoplastic Nylon Denture. Proceeding Book The snd International Joint Symposium on Oral and dental Science in Conjuction with Dental Specialists seminar.h. 281- 285.

Ismiyati, T., dan Setyahadi, S., 2014 Antifungal of Thermoplastic Nylon Denture Base Plate Incorporate with Nanoparticles High-Density Chitosan, Journal of Chitin and Chitosan Sciences, Vol 2, h. 1-7.

ISO10993-5, 2009, Biological Evaluation of Medical Devices-Part 5 : Test for in vitro cytotoxicity, h.28.

Junqueira, L.C., dan Carneiro, C., 2005, Basic Histology: Text and atlas, 11th ed., Sao Paulo: Mc Graw Hill, h.502.

Kuehnel, W., 2003, Color atlas of cytology, histology, and microscopic anatomy, Thieme, USA, h.268-270.

McCabe,F., walls, W.G., 2008, Intelligent Chitosan –based hydrogels as Multifunctional materials, dalam Shahinpoor, m., Schneider, H.(eds), Intellegent Materials, Royal Society of Chemical, Cambride, h, 448

Paranhos,H.F., Silva,C.H., Souza,R.F., Cruz,P.C., Freitas,K.M., Peracini,A., 2007, Effect of Mechanical & Chemical Methodes on Denture Biofilm Accumulation, J. Oral Rehabil 34 (8) : 607-12

Portner, R., 2007, Animal cell biotechnology: methods and protocols, 2nd ed, New Jersey: Humana Press Inc, h.212-214

Rasmussen,R.S., dan Morrisey,M.T., 2008, Chitin and Chitosan, marine Nutraceuticals nd Function Food, CRC Press, New York

Sakaguci R.L., dan Power J.M., 2012, Craig’s Restoratie Dental Materials, 13th ed., Elsevier Mosby, Philadelphia p.162-91

Salerno,C., Pascale.M., Contaldo,M., Esposito,., Busciolano,M., Millilo, L., Guide,A., Petruzzi,M., Serpico, L., 2011, Candida-Associated denture Stomatitis, Med Oral Patol Oral Cir Bucal 16 (2) : 139 – 43

Schmal G., dan Arenholt B.D., 2009, Biocompatibility of Dental materials, Springer, New York, h. 13-17, 99- 111

Tikhonov, V., E., Stepnova, E.A., Babak, V.G., Yamskov, I.A., Palma-Guerrero,J., Jansson, h., Lopez-Llorca, L.V., Salinas, J., Gerasimenko, D.V.,Avdienko,I.D., Varlamov, V.P., 2006, Bactericidal and Antifungal antivities of a Low Molecular Weight Chitosan and Its N-/2(3)-(Dodec-2-Enyl)Succinoyl/-Derivatives,Carbohyd, polym, 64 ; 66 – 72.



DOI: https://doi.org/10.22146/teknosains.27580

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