Efek antibakteri dan penghambatan biofilm ekstrak sereh (Cymbopogon nardus L.) terhadap bakteri Streptococcus mutans

https://doi.org/10.22146/majkedgiind.9120

Zwista Yulia Dewi(1*), Asikin Nur(2), Triana Hertriani(3)

(1) Program Studi Magister Ilmu Kedokteran Gigi, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Bagian Biomedika Kedokteran Gigi, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Bagian Biologi Farmasi, Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


Antibacterial effect and biofilm inhibition Of Lemongrass extract (Cymbopogon nardus L.) against the growth of Streptococcus mutans. Caries prevention can be carried out by several methods. One of them is by controlling the plaque accumulation on the surface of the teeth. Lemongrass (Cymbopogon nardus L) is containing certain compound that can inhibit the growth of bacteria and biofilm. The objective of this research is to observe the influence of antibacterial and biofilm inhibition of lemongrass extract against the growth of S. mutans. Subjects were S. mutans bacteria on KHM90 test as much as 6x108 CFU/ml and on biofilm inhibition test as much as 15x108 CFU/ml. Lemongrass was extracted using petroleum ether followed by using 70% ethanol. Antibacterial activity test carried out with KHM90 determination test using microdilution method on microplate flat bottom 96 wells. Bacteria were prepared by making a suspension in NB media and adjusted to McFarland II standard (6x108 CFU/ml). Biofilm inhibition activity test was performed using microdilution method of the biofilm formed on microplate flat flexible PVC U-bottom 96 wells which were stained using 1% of crystal violet. Bacteria were prepared by making a suspension in BHI media and adjusted to McFarland V standard (15 x108 CFU/ml). The result in the form of optical density (OD) was read by Bio-rad microplate reader Benchmark at a wavelength of 595 nm. The value of IC50 was determined by probit method using SPSS version 15.The results of this study of measurements on KHM90 test showed that 108,36% w/v is capable of inhibiting the growth of bacteria. Biofilm inhibitory activity showed IC50 lemongrass value was 0,137% w/v. The conclusion of this study is that lemongrass extract has antibacterial effect against bacteria S. mutans showed by KHM90 obtained at concentrations of 0,18% w/v and there is lemongrass extract biofilm inhibitory effect against the bacteria S. mutans indicated by IC50 value 0,137%


Keywords


lemongrass; Streptococcus mutans; antibacteria; biofilm inhibition; teeth’s plaque

Full Text:

PDF


References

Shakh MAR, Ohara-Nemoto T, Ono Y, Shimoyama S, Kimura TK,

Nemoto K. In vitro processing of glutamylendopeptidaseproenzymes from Enterococcus faecalisand importanceof N-terminal residue in enzyme catalysis, Advances in Biochemistry. 2013; 1(5): 73-80.

Sterer N, Rosenberg M. Oral malodor management, breath odors, springer-verlas berlin heidelberg. 2011. H. 18-25.

Darby ML, Margaret MW. Dental hygiene: theory and practice, 3rd ed. Elsevier, London. 2010. H. 3,16.

Madigan MT, Martinko JM, Brock TD. Brock Biology of Microorganisms. 14th Ed. New Jersey: Pearson Prentice. 2014: 617-619.

Eke PI, Dye BA, Wei L. Thornton-Evans GO, and Genco RJ. Prevalence of periodontitis in adults in the United States: 2009 and 2010. J Dent Res. 2012; 91(10): 914-20.

Arinda A, Rahardjo P, Triwardani A. Perbedaan efektivitas obat kumur yang mengandung cengkeh dengan obat kumur chlorexidine gluconat 0.2% dalam menghambat pembentukan plak. Orthodontic Dental Journal. 2012; 01(01): 22-25.

Diah K. Kontrol plak kemikal dalam pencegahan gingivitis dan periodontitis. Periodontic Journal. 2012; 01(02): 1-6.

Raina L. Ensiklopedi tumbuhan berhasiat obat. Jakarta: Salemba Medika. 2011; 13-19.

Bassolé I HN, Lamien-Meda A, Bayala B, Obame LC, Ilboudo AJ, Franz C, Novak J, Nebié RC, Dicko R. Chemical composition and antimicrobial activity of Cymbopogoncitratus and Cymbopogongiganteusessential oils alone and in combination. Phytomedicine. 2011; 18: 1070-1074.

Jafari B, Amirreza E, Babak MA, Zarifeh H. Antibacteria Activities of Lemon Grass Methanol Extract and Essence Pathogenic Bacteria. American-Eurasian J. Agric and EnvironSci. 2012; 12(8): 1042-1046.

Reveny J. Daya antimikroba ekstrak dan fraksi daun sirih merah (Piper betle Linn.). Sumatra Utara: Jurnal Ilmu Dasar. 2011; 12(1): 6-12.

Astuti SM. Skrining fitokimia dan uji aktifitas antibiotika ekstrak etanol daun, batang, bunga dan umbi tanaman binahong (anredera cordifolia (ten) steenis. Balai Besar Pengujian Mutu Dan Sertifikasi Obat Hewan (BBPMSOH). Bogor. Dan Fakulti Kejuteraan

Kimia, Universiti Malaysia Pahang. Pahang. 2011.

Meena AK, Goyal R, Subraya BG, Kamath S, Anand KM, Aggrawal M. A novel anti-oxidant lemon grass oil mouth wash - a clinical trial.

Asian J ExpBiol Sci. 2011; 2(3): 482-6.

Radafshar G, Mahboob F, Kazemnejad E. A study to assess the plaque inhibitoryaction of herbal-based tooth paste: a double blind

controlled clinical trial. Journal of Medicinal Plant Research. 2010; 4(12): 1182-6.

Rosyita A. Formulasi dan studi efektivitas sediaan gel antiseptik tangan dari minyak atsiri daun kemangi (Ocimum sanctum Linn.) terhadap jumlah bakteri tangan siswa SD Kandang Sapi Surakarta sebagai solusi alternatif pencegahan penyakit diare, PKM-P, Fakultas Farmasi, Universitas Sebelas Maret. 2012.

Bhowmik N. Dental plaque “Unveiling the biofilm inside”. e-Journal of Dent. 2012; 2(1): 119.

Tortora J, Gerard J, Funke BR, Case CL. Microbiology an introduction. 10th Edition. Pearson Education. San Fransisco. 2010:

-301.

Osso D, Kanani N. antiseptic mouthrinses: an update on comparative effectiveness, risks and recommendations. The Journal of Dental Hygiene. 2013; 87(1): 12.

Chetrus V, Ion IR. Dental plaque-Classification, formation and identification. Int Jof Medical Dent, 2013; 3(2): 139-43.

Miletis I, Baraba A. Aetiological factors for susceptibility: the location (number, location, activity) and the plaque (identification tools, scoring). J of Minimum Intervention in Dent, 2011; 4(2): 13-6.

Rinidar M, Isa T, Armansyah. Nilai Inhibition Concentration (IC50) Ekstrak Metanol Daun Sernai (Wedelia biflora). Jurnal Medika Veterinaria. 2013; 7: 8-12, 0853-1943.

Madhuri S, Gopind P, Karuna SV. Antioxidant, immunomodulatory and anticancer activities of emblicaofficinalis: an overview. International Research Journal of Pharmacy. 2011; 2(8): 38-42.

Agoes A. Tanaman obat Indonesia. Salemba Medika, Jakarta. 2010: 55.

Kukreja BJ, Dodwad V. Herbal mouthwashes-a gift of nature. Int J Pharm Bio Science. 2012; 3(2): 46-52.



DOI: https://doi.org/10.22146/majkedgiind.9120

Article Metrics

Abstract views : 6847 | views : 12610

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Majalah Kedokteran Gigi Indonesia




Currently, Majalah Kedokteran Gigi Indonesia indexed by:

         

 

 

 

 

 

 View My Stats


real
time web analytics