Effectiveness of vitamin D3 supplementation with protein realimentation in osteoblast and osteoclast of the maxilla of breastfed malnourished rat infants

Riski Amalia Hidayah; Aris Aji Kurniawan; Pratiwi Nur Widyaningsih; Rakhmawati Rakhmawati

doi:10.22146/majkedgiind.78293

Effectiveness of vitamin D3 supplementation with protein realimentation in osteoblast and osteoclast of the maxilla of breastfed malnourished rat infants

https://doi.org/10.22146/majkedgiind.78293

Riski Amalia Hidayah^(1*), Aris Aji Kurniawan⁽²⁾, Pratiwi Nur Widyaningsih⁽³⁾, Rakhmawati Rakhmawati⁽⁴⁾

(1) Department of Dentistry, Faculty of Medicine, Jenderal Soedirman University, Purwokerto, Central Java
(2) Department of Dentistry, Faculty of Medicine, Jenderal Soedirman University, Purwokerto, Central Java
(3) Department of Dentistry, Faculty of Medicine, Jenderal Soedirman University, Purwokerto, Central Java
(4) Department of Dentistry, Faculty of Medicine, Jenderal Soedirman University, Purwokerto, Central Java
(*) Corresponding Author

Abstract

Child malnutrition can happen because of lack of nutrition during pregnancy. Malnutrition can cause low birth weight in babies. This can cause jaw and alveolar bone abnormalities that can lead to malocclusion, periodontitis, and others. This condition can affect bone cells, such as osteoblast and osteoclast. Vitamin D3 supplementation accompanied by protein realimentation may promote optimum bone growth. This research aims to investigate the effect of protein realimentation and vitamin D3 on osteoblast and osteoclast of malnourished rat pups’ maxilla. In this research, the rat pups were divided into 5 groups: a positive control group (K+), a negative control group (K-), and 3 treatment groups (P1, P2, and P3). The subjects of this research were 10 male and 30 female Wistar rats. A condition of malnutrition was induced in the rat pups and they were given vitamin D3 supplementation with protein realimentation. Rat pups in each group were euthanized on the 22nd day to observe the number of osteoclast and osteoblast in the maxilla. The results showed that the number of osteoblasts between normal and malnourished rats was significantly different (p value < 0.05), but the number of osteoclasts between rats in the control and treatment groups was not significantly different. Administration of vitamin D3 may affect the number of osteoblasts and may not affect the number of osteoclasts on bone. The effective dose of vitamin D3 is 0.36 IU/day.

Keywords

osteoblast; osteoclast; protein; vitamin D3

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References

1. Ningtyas FW, Sulistiyani, R LY, Rohmawati N. Gizi dalam Daur Kehidupan. Jember: UPT Percetakan dan Penerbitan Universitas Jember; 2020.

2. da Silva Lopes K, Ota E, Shakya P, Dagvadorj A, Balogun OO, Peña-Rosas JP, De-Regil LM,
Mori R. Effects of nutrition interventions during pregnancy on low birth weight: An overview of
systematic reviews. BMJ Global Health. 2017; 2(3): 1–11. doi: 10.1136/bmjgh-2017-000389

3. Bonjour J-P. The dietary protein, IGF-I, skeletal health axis. Horm Mol Biol Clin Investig. 2016; 28(1): 39–53. doi: 10.1515/hmbci-2016-0003

4. Alexander BT, Dasinger JH, Intapad S. Effect of low birth weight on women’s health. Clin
Ther. 2014; 36(12): 1913–1923. doi: 10.1016/j.clinthera.2014.06.026

5. Premkumar S. Textbook of Orthodontics. New Delhi: Elsevier; 2015. 53–54.

6. Bozzini C, Champin GM, Bozzini CE, Alippi RM. Growth inhibition in rats fed inadequate
and incomplete proteins: repercussion on mandibular biomechanics. Acta Odontol Latinoam. 2013; 26(1): 43–53.

7. Pudyani PS. Reversibilitas kalsifikasi tulang akibat kekurangan protein pre dan post natal
(Reversibility of bone calcification on pre and post natal protein deficiency). Dent J (Majalah
Kedokt Gigi). 2005; 38(3): 115-119. doi: 10.20473/j.djmkg.v38.i3.p115-119

8. Murray RK, Granner DK, Rodwell VW. Biokimia Harper, Edisi ke-29. Jakarta: EGC;
2017.

9. Guyton AC, Hall JE. Buku Ajar Fisiologi Kedokteran Edisi XII. Jakarta: EGC; 2016.

10. Garniasih D, Djais JTB, Garna H. Hubungan antara kadar albumin dan kalsium serum
pada sindrom nefrotik anak. Sari Pediatri. 2016; 10(2): 100–105. doi: 10.14238/sp10.2.2008.100-5

11. Hou Y-C, Wu C-C, Liao M-T, Shyu J-F, Hung C-F, Yen T-H, Lu C-L, Lu K-C. Role of
nutritional vitamin D in osteoporosis treatment. Clin Chim Acta. 2018; 484: 179–191.
doi: 10.1016/j.cca.2018.05.035

12. Yolanda N. Perlukah Suplemen Vitamin D? Ikat Dr Anak Indones [Internet]. 2016;
Available from: https://www.idai.or.id/artikel/klinik/pengasuhan-anak/perlukah-suplemenvitamin-d

13. Thiele DK, Senti JL, Anderson CM. Maternal vitamin D supplementation to meet the needs
of the breastfed infant: a systematic review. J Hum Lact. 2013; 29(2): 163–170.
doi: 10.1177/0890334413477916

14. Hollis BW, Wagner CL. Vitamin D requirements during lactation: high-dose maternal
supplementation as therapy to prevent hypovitaminosis D for both the mother and the nursing infant. Am J Clin Nutr. 2004; 80(6): 1752S-1758S. doi: 10.1093/ajcn/80.6.1752S

15. Sotunde OF, Laliberte A, Weiler HA. Maternal risk factors and newborn infant vitamin D
status: a scoping literature review. Nutr Res. 2019; 63: 1–20.
doi: 10.1016/j.nutres.2018.11.011

16. Ghasemi, Asghar; Sajad JKK. Review article : The laboratory rat : age and body weight
matter. Excli J. 2021; 20: 1431-1445. doi: 10.17179/excli2021-4072

17. Xie Z, Dong Q, Ge J, Chen P, Li W, Hu J. Effect of low birth weight on impaired renal
development and function and hypertension in rat model. Ren Fail. 2012; 34(6): 754–759.
doi: 10.3109/0886022X.2012.676526

18. Hassan EK, Hegazy AM. Histopathological and weight changes of the rat fetal liver
of females fed with low protein diet during pregnancy. AAMJ. 2014; 2(12): 257–272.

19. Syari M, Serudji J, Mariati U. Peran asupan zat gizi makronutrien ibu hamil terhadap berat
badan lahir bayi di Kota Padang. J Kesehat Andalas. 2015; 4(3): 729-736.
doi:10.25077/jka.v4i3.355

20. Cunningham FG, Leveno KJ, Bloom SL, Spong CY, Dashe JS. Williams obstetrics,
24e. New York, NY, USA: Mcgraw-hill; 2014.

21. Izzati RF, Mutalazimah M. Energy, protein intake, and chronic energy deficiency in
pregnant women: a critical review BT. Proceedings of the International Conference
on Health and Well-Being (ICHWB 2021). In Atlantis Press. 2022.
doi: 10.2991/ahsr.k.220403.010

22. Nelwan SC, Tedjosasongko U, Kuntari S, Utomo DH, Saskianti T, Puteri MM, Dharmawan
D, Fitriani Y, Kurnia PA, Mufida AR, Fajrin NZ, Rahayu RP, Nowwarote N. The benefits of the
combination of vitamin D3, K2 supplements, and UV-B exposure for increasing bone
density: a simple solution for bone health. Journal of International Dental and Medical
Research. 2021; 14(4): 1453–1458.

DOI: https://doi.org/10.22146/majkedgiind.78293