* Corresponding Author
Effectiveness of Aspergillus sp. extract in denture adhesive on surface roughness of acrylic resin on Candida albicans biofilm formation
https://doi.org/10.22146/majkedgiind.64485 Okmes Fadriyanti(1*), Ismet Danial Nasution(2), Widowati Siswomihardjo(3), Dian Handayani(4)
(1) Department of Prosthodontics, Faculty of Dentistry, Baiturrahmah University, Padang, West Sumatra
(2) Department of Prosthodontics, Faculty of Dentistry, Sumatera Utara University, Medan, North Sumatra
(3) Department of Dental Materials, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(4) Sumatra Biota Laboratory, Faculty of Pharmacy, Andalas University, Padang, West Sumatra
(*) Corresponding Author
Abstract
The denture adhesive increases retention on the denture base and affects oral microorganisms. Adding antifungals to denture adhesives can inhibit the Candida albicans biofilms formation and prevent denture stomatitis. The combination of denture adhesives and herbal medicines is an alternative to antifungals, which have few side effects because it is a plant. Moreover, one of them is the endophytic Aspergillus sp. extract containing chemical compounds that can inhibit the Candida albicans biofilms formation. This study aims to analyze the effectiveness of the endophytic Aspergillus sp. extract in denture adhesive materials for Candida albicans biofilm formation on acrylic resin surfaces. The research method is to extract the Aspergillus sp. extract antibiofilm test. Denture adhesive formulation was adjusted to the standard, and added Aspergillus sp. with concentrations of 3.125%, 6.25%, 12%, and 25%. The research sample used hot polymerized acrylic resin. The control group used X denture adhesive and added nystatin, each group suspended by Candida albicans for 24, 48, and 72 hours. Examination of biofilm formation activity on the surface of acrylic resin used SEM. The analysis used Two Way Anova. Aspergillus sp. extract in denture adhesive effectively prevents Candida albicans biofilm formation within 24 hour incubation time. In conclusion, extract of the endophytic Aspergillus sp. in denture adhesive can inhibit the formation of Candida albicans biofilm on the surface roughness of acrylic resin.
Keywords
Full Text:
PDFReferences
1. Central Bureau of Statistics. Indonesia Population Projection 2015-2035. Jakarta;
2013. 26.
2. Power JM, Sakaguci RL. Craig's Restorative Dental Materials, 13th ed. Philadelphia:
Elsevier Mosby; 2012. 162-191.
3. Duqum I, Powers KA, Cooper L, Felton D. Denture adhesive use in complete dentures:
Clinical recommendations and review of the literature. Gen Dent. 2012; 60(6): 467-477.
4. Thalib B, Alwi PI. The comparison of body mass index of elderly used and did not use
full denture. Journal of Dentomaxillofacial Science. 2011; 10(3): 140-143.
doi: 10.15562/jdmfs.v10i3.272
5. Zarb G, Hobkirk J, Eckert S, Jacob R. Prosthodontic treatment for edentulous
patients: Completed denture and implant – supported prosthesis. 13th ed. Mosby:
Elsevier; 2012. 133-47, 155-59.
6. Makhira S, Nikawa H, Satonobu SV, Hamada T. Growth of Candida species on commercial
denture adhesives in vitro. Int J Prosthodont. 2001: 14(1); 48-52.
7. Nunes EM, Policastro VB, Scavassin PM, Leite AR, Medonza, Medonza, MDO, Giro G,
Junior NmdeO, Compagnoni MA, Pero AC. Crossover clinical trial of different methods
of removing a denture adhesive and the influence on the oral microbiota. J Prosthet
Dent. 2016; 115(4): 465-468. doi: 10.1016/j.prosdent.2015.08.004
8. Rajaram A, Manoj SS. Influence of 3 different forms of a commercially available denture
adhesive material on the growth of Candida species: An in vitro study. J Prosthet Dent.
2017; 118(3): 379-385. doi: 10.1016/j.prosdent.2016.11.015
9. Jain P, Sikka R, Arora D, Khatri M. Denture adhesive from then now. Journal of the Dental
Herald. 2015; 1(2): 1-3.
10. Kumar PR, Shajahan PA, Mathew J, North KA, Araind P, Ahammed MF. Denture adhesive in
prosthodontics: an overview. J Int Oral Health. 2015; 7(1): 93-95.
11. Cartagana AF, Esmerino LA, Polak-Junior R, Parreiras SO, Michel MD, Farago PV,
Campanha NH. New denture adhesive containing miconazole nitrate polymeric
microparticle: antifungal, adhesive force and toxicity properties. Dent Mater. 2017; 33(2):
53-61. doi: 10.1016/j.dental.2016.09.039
12. Da Silva, WJ, Rached RN, Rosalen PL, Del bel Cury AA. Effects of nystatin, fluconazole and
propolis on poly(methyl methacrylate) resin surface. Braz Dent J. 2008; 19(3): 190–196.
doi: 10.1590/s0103-64402008000300003
13. Almeida NLM, Saldanha LL, da Silva RA, Pinke KH, da Costa EF, Porto VC, Dokkedal
AL, Lara VS. Antimicrobial activity of denture adhesive associated with equisetum
giganteum and punica granatum-enriches fraction against Candida albicans biofilms on
acrylic resin surfarce. Biofouling. 2018; 34(1): 62-73. doi: 10.1080/08927014.2017.1407408
14. Strobel GA, Daisy B. Bioprocessing for microbial endophytes and their natural
products. Microbiol Mol Biol Rev. 2003; 67(4): 491-502.
doi: 10.1128/MMBR.67.4.491-502.2003
15. Zhao J, Wang T, Shan T, Zhong L, Liu X, Gao L. Endophytic fungi for producing bioactive
compounds originally from their host plants. In Current Research, Technology and Education
Topics in Applied Microbiology and Microbial Technology. Mendez-Vilaz A. (ed). Formatex.
2010; 567-576.
16. Rival H, Handayani D, Rasyid R. Screening of antimicrobial and cytotoxic activities of
endophytic fungi isolated from mangrove plant Rhizophora mucronata Lam. Int Journal of Pharmaceutical Science and Medicine (IJPSM). 2018; 3(3): 9-20.
17. Gani BA, Bachtiar EW, Bachtiar BM. The role of cigarettes smoke condensatein enhanced
Candida albicans virulence of salivary isolates based on time and temperature. Journal of International Dental and Medical Research. 2017; 10(9): 769-777.
18. Silva MJ, de Oliveira DG, Marcillo OO, Neppenlenbroek KH, Lara VS, Porto VC. Effect
of denture-coating composite on Candida albicans biofilm and surface degradation after
disinfection protocol. Int Dent J. 2016; 66(2): 86-92. doi: 10.1111/idj.12212
19. Ibraheem MEMA, Hammad HG. Effect of commercially available denture adhesive
on microhardness of a flexible denture base material. Open Access Maced J Med Sci. 2019;
7(5): 862-868. doi: 10.3889/oamjms.2019.193
20. Gulati M, Lohse, MB, Ennis C, Gonzalez RE, Perry AM, Ayah P, Nobile CJ. In vitro culturing
an screening of candida albicans biofilms. Curr Protoc Microbiol. 2018; 50(1): e60. doi:
10.1002/cpmc.60
21. Serrano-Fujarte I, López-Romero E, Reyna-López GE, Martínez-Gámez MA, VegaGonzález A, Cuéllar-Cruz M. Influence of culture media on biofilm formation by Candida species and response of sessile cells to antifungals and oxidative stress. Biomed Res Int. 2015; 2015: 783639.
doi: 10.1155/2015/783639
22. Baboni FB, Guariza FO, Moreno AN, Rosa EAR. Influence of cigarette smoke condensate
on cariogenic and candidal biofilm formation on orthodontic materials. Am J Orthod
Dentofacial Orthop. 2010; 138(4): 427-434. doi: 10.1016/j.ajodo.2009.05.023
23. Barriuso J. Quorum sensing mechanisms in fungi. AIMS Microbiology. 2015; 1(1): 37-47.
doi: 10.3934/microbiol.2015.1.37
24. Nobile CJ, Johnson AD. Candida albicans biofilms and human disease. Annu Rev
Microbiol. 2015; 69(1): 71-92. doi: 10.1146/annurev-micro- 091014-104330
25. Aksoy M, Gülçin, Küfrevioğlu Öİ. In vitro antioxidant profiles of some flavonoids. AIP
Conference Proceedings. 2016; 1726(1): 020103. doi: 10.1063/1.4945929
26. Mishra AK, Mishra A, Kehri HK, Sharma B, Pandey AK. Inhibitory activity of Indian spice
plant Cinnamomum zeylanicum extracts against Alternaria solani and Curvularia
lunata, the pathogenic dematiaceous moulds. Ann Clin Microbiol Antimicrob. 2009; 8(1): 9.
doi: 10.1186/1476-0711-8-9
27. Lamont RJ, Koo H, Hajishengallis G. The oral microbiota: dynamic communities and
host interactions. Nat Rev Microbiol. 2018; 16(12): 745-759. doi: 10.1038/s41579-018-
0089-x.
28. Pöllänen MT, Paino A, Ihalin R. Environmental stimuli shape biofilm formation and the
virulence of periodontal pathogens. Int J Mol Sci. 2013; 14(8): 17221-17237.
doi: 10.3390/ijms140817221
29. Nickerson KW, Atkin AL, Hornby JM. Quorum sensing in dimorphic fungi: farnesol and
beyond. Appl Environ Microbiol. 2006; 72(6): 3805-3813. doi: 10.1128/AEM.02765-05
30. Cornet M, Gaillardin C. pH signaling in human fungal pathogens: a new target for antifungal
strategies. Eukaryot Cells. 2014; 13(3): 342-352. doi: 10.1128/EC.00313-13
31. Mehmood A, Liu G, Wang X, Meng G, Wang C, Liu Y. Fungal quorum-sensing molecules and inhibitors with potential antifungal activity: a review. 2019; 24(10): 1950.
doi: 10.3390/molecules24101950
32. Dongari-Bagtzoglou A, Kashleva H, Dwivedi P, Diaz P, Vasilakos J. Characterization of
mucosal Candida albicans biofilms. PLoS One. 2009; 4(11): e7967.
doi: 10.1371/journal.pone.0007967
33. Vojdani M, Giti R. Polyamide as a denture base material: A literature review. J Dent
(Shiraz). 2015; 16(1 Suppl): 1-9.
34. Ganguly S, Mitchell AP. Mucosal biofilms of Candida albicans. Curr Opin Microbiol. 2011;
14(4): 380-385. doi: 10.1016/j.mib.2011.06.001
35. Al-Fouzan AF, Al-mejrad LA, Albarrag AM. Adherence of Candida to complete denture
surfaces in vitro: A comparison of conventional and CAD/CAM complete dentures. J Adv
Prosthodont. 2017; 9( 5): 402-408. doi: 10.4047/jap.2017.9.5.402
36. Androetti AM, De Sausa CA, Goaito MC. In vitro evaluation of microbial adhesion on the
different surface roughness of acrylic resin specific for ocular prosthesis. Eur Dent. 2018;
12(2): 176-183. doi: 10.4103/ejd.ejd_50_18
DOI: https://doi.org/10.22146/majkedgiind.64485
Article Metrics
Abstract views : 1423
| 
views : 1017
Refbacks
- There are currently no refbacks.
Copyright (c) 2023 Majalah Kedokteran Gigi Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.