Effects of sisal nanofiber addition to epoxy resin-based sealer on its antibacterial power against Enterococcus faecalis


Heny Retno Mayangsari(1), Tunjung Nugraheni(2), Ema Mulyawati(3*)

(1) Conservative Dentistry Specialty Program, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(2) Department of Conservative Dentistry, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(3) Department of Conservative Dentistry, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author


Sealer is one of root canal filler materials which has been developed and has an antibacterial agent to keep root canal sterile during and after an obturation process. This study aimed to find out the effect of sisal nanofiber addition to resin epoxy-based sealer on the antibacterial power against Enterococccus faecalis (E. faecalis) which is known as adaptive and potent bacteria which can be both aerobic and anaerobic. Sisal fibers were processed through many stages to make it nano sized (scouring, bleaching, neutralization, ultrasonification, and freeze-drying). Once nano-sized sisal fibers had been obtained, they were then mixed with sealer powder (AH26) in different concentrations: powder 0%, 0.25%, 0.5%, 0.75% and 1%. These concentrations were chosen based on preliminary research for reasonable contact angle measurement of sisal-sealer mixture. Antibacterial effect was examined using the diffusion method, each concentration was tested in 5 petri dishes which were planted with 1.5 X 108 CFU/ml E.faecalis bacteria. Each dish consisted of 5 holes (6 mm in diameter), each hole represented each concentration of nano sisal and sealer which were mixed until homogenous for 3 minutes before added to each hole. The dishes were then incubated for 48 hours at 37 °C. Inhibitory zones were measured, and analyzed using one-way ANOVA. The one-way ANOVA result showed that p=0.502 (p>0.05), meaning that the sisal nanofiber addition to epoxy resin-based sealer had no effect on E.faecalis inhibition. Thus, there was no effect of sisal nanofiber addition to epoxy resin-based sealer on E. faecalis.


antibacterial; Enterococcus faecalis; epoxy resin-sealer; sisal nanofiber

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1. Fejerkov O, Kidd E. Dental Caries, 2nd Ed. Blackwell Munksgaard Publishing Company;

2. Camargo CHR, Camargo SEA, Valera MC, Hiller KA, Schmalz G, Schweikl H. The
induction of citotoxicity, oxidative stress, and genotoxicity by root canal sealers in mammalian cells. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology. 2009; 108(6): 952-960. doi: 10.1016/j.tripleo.2009.07.015.

3. Basuki T, Verona L. Manfaat serat sisal (Agave sisalana L) dan Bambu (Bambusoideae)
untuk memenuhi kebutuhan masyarakat modern. Agrika Jurnal Ilmu-ilmu Pertanian.
2017; 11(2): 123-134.

4. Ade-Ajayi AF, Hammuel C, Ezeayanaso C, Ogabiela EE, Udiba UU, Anyim B, Olabanji O.
Preliminary phytochemical and antimicrobial screening of agave sisalana perrine juice (waste). Journal of Environmental Chemistry and Ecotoxicology. 2011; 3(7): 180-183.

5. Razmi H, Yazdi KA, Jabalameli F, Parvizi S. Antimicrobial effects of AH26 sealer/antibiotic
combination against enterococcus faecalis. Iran Endod J. 2008; 3(4): 103-108.

6. Beyth N, Shvero DK, Zaltsman N, Haddad YH, Abramovitz I, Davidi MP, Weiss EI. Rapid kill
– novel endodontic sealer and enterococcus faecalis. PLoS One. 2013; 8(11).
doi: 10.1371/journal.pone.0078586.

7. Pasril Y, Yuliasanti A. Daya antibakteri ekstrak daun sirih merah (Piper Crocatum) terhadap
bakteri enterococcus faecalis sebagai bahan medikamen saluran akar dengan metode
dilusi. IDJ: 2014; 3(1): 88-95.

8. Nugroho DA. Uji kekuatan fleksural, kekuatan tekan dan perlekatan bakteri s.mutans
pada bahan tumpatan (tambalan gigi) resin komposit dengan nanosisal sebagai filler (bahan pengisi). Universitas Muhammadiyah Yogyakarta: Disertasi; 2018.

9. Ingle JI, Leif KB, Baumgartner JC. Ingle’s Endodontics, 6th Ed. Mosby: BC Decker,
Ontario; 2008.

10. Joseph K, Filho RDL, James B, Thomas S, Carvalho LH. A review on sisal fiber reinforced
polymer composites. Rev Bras Eng Agric Ambient. 1999; 3(3): 367-379.
doi:10.1590/1807-1929/agriambi v3n3p367-379

11. Zhou H, Shen Y, Zheng W, Li L, Zheng Y, Haapasalo M. Physical properties of 5 root
canal sealers. Journal of Endodontics. 2013; 39(10): 1281-1286.
doi: 10.1016/j.joen.2013.06.012

12. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999; 12(4): 564-
582. doi: 10.1128/CMR.12.4.564

DOI: https://doi.org/10.22146/majkedgiind.62867

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