Effect of collagen-chitosan hydrogel formula combined with platelet-rich plasma (A study of ph, viscocity, and swelling test)
Tutut Prabantari Anindyajati(1), Sri Pramestri Lastianny(2), Flandiana Yogianti(3), Kwartarini Murdiastuti(4*)
(1) Clinical Dentistry Magister Program, Periodontology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(2) Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(3) Department of Dermatology and Verology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta
(4) Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author
Abstract
Platelet-rich plasma (PRP) contains growth factors that speed up a healing process. The liquid form of PRP is difficult to be applied, so it needs material as a carrier of collagen. Collagen can be used as carrier materials and capable to activate growth factors and increase the concentration of the PRP to become a gel. Collagen often losses its shape and size due to degradation when exposed to body fluids. In the selection of materials, collagen
can synthesize with chitosan. Collagen-chitosan hydrogel has potential as scaffold. Many formulations for proper hydrogel applied in the oral cavity need to pay more attention to several conditions, such as a neutral pH, high viscosity and ideal swelling. The aims of this study was to examine the influence of various formulations of collagenchitosan hydrogel PRP
against pH, viscosity, and swelling. The study samples consisted of 25 collagen-chitosan
hydrogel formulations. The samples were divided into five groups: 100/0; 75/25; 50/50; 25/75; 0/100. The first stage of the test was pH, viscosity and swelling measurement of the collagen-chitosan hydrogel to determine which ones matched the criteria. Collagen-chitosan hydrogel formulations were added with PRP with a ratio of 1:1. The second stage was to measure the pH, viscosity, and swelling measurement to see the results of the physical parameters of the hydrogel after mixed with PRP. The observation data were analyzed by t-test for pH and viscosity, while twoway ANOVA and post hoc LSD for swelling test. The results showed that collagen-chitosan hydrogel formulations of 25/75 and 0/100 met the criteria of pH, viscosity, and swelling. The pH of both groups showed no significant difference (p>0.05), but the viscosity and swelling variables showed significant difference (p<0.05). To conclude, collagen-chitosan hydrogel formulations mixed with PRP had no effect on pH, but had an effect on viscosity and swelling test. The collagen-chitosan hydrogel formulations mixed with PRP which matched the criteria were 25/75 and 0/100.
Keywords
Full Text:
PDFReferences
1. Arbildo H, Gamarra L, Rojas S, Infantes E, Lamas C, Vasquez H. Clinical effect of plateletrich plasma in the treatment of periodontal infrabony defect. Systematic review and metaanalysis. Journal of Oral Research. 2017; 6(4): 97-104. doi: 10.17126/joralres.2017.028
2. Fufa D, Shealy B, Jacobson M, Kevy S, Murray MM. Activation of platelet-rich plasma using soluble type i collagen. J Oral Maxillofac Surg. 2008; 66(4): 684-690. doi: 10.1016/j.joms.2007.06.635
3. Harrison S, Vavken P, Kevy S, Jacobson M, Zurakowski D, Murray MM. Platelet activation
by collagen provides sustained release of anabolic cytokines. Am J Sports Med. 2011;
39(4): 729-734. doi: 10.1177/0363546511401576
4. Rosdiani AF, Widiyanti P, Rudyarjo DI. Synthesis and characterization biocomposite
collagen-chitosan- glycerol as scaffold for gingiva recession therapy. J Int Dent Med
Res. 2017; 10(1): 118-122.
5. Deng C, Zang P, Vulesevic B, Kuraitis D, Li F, Yang AF, Griffith M, Ruel M, Suronen E.
A Collagen-chitosan hidrogel for endothelial differentiation and angiogenesis. Canada.
Tissue Eng Part A. 2010; 16(10): 3099-3109. doi: 10.1089/ten.tea.2009.0504
6. Hui E, Lerouge S. Mechanical and biological properties of chitosan/purecol collagen
hidrogels. J of Rheology. 2017; 30(2): 1-9.
7. Alsarra IA. Chitosan topical gel formulation in the management of burn wounds. Int J Biol
Macromol. 2009; 45(1): 16-21. doi: 10.1016/j.ijbiomac.2009.03.010
8. Baliga S, Muglikar S, Kale R. Salivary pH: A diagnostic biomarker. J Indian Soc Periodontol. 2013; 17(4): 461–465. doi: 10.4103/0972124X.118317
9. Danimayostu A. Pengaruh penggunaan pati kentang (Solanum tuberosum) termodifikasi
asetilasi-oksidasi sebagai gelling agen terhadap stabilitas gel natrium diclofenak. Pharmaceutical Journal of Indonesia. 2017; 3(1): 25-32.
10. Ibrahim A. Formulasi dan optimasi basis gel HPMC (Hidroxy Propyl Methyl Cellulose)
dengan berbagai variasi konsentrasi. Journal of Tropical Pharmacy and Chemistry. 2015;
3(2): 101-108. doi: 10.25026/jtpc.v3i2.95
11. Zhang F, Wang A, Li Z, He S, Shao L. Preparation and characterisation of collagen
from freshwater fish scales. Food and Nutrition Sciences. 2011; 2: 818-823. doi: 10.4236/
fns.2011.28112
12. Yan LP, Wang YJ, Ren L, Wu G, Caridade SG, Fan JB, Reis RL. Genipin-cross-linked
collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering
applications. J Biomed Mater Res A. 2010; 95(2): 465–475. doi: 10.1002/jbm.a.32869
13. Pati F, Datta P, Chatterjee J, Dhara S, Adhikari B. Development of chitosan-tripolyphosphate fiber for biomedical application. Kharagpur: IEEE Students’ Technology Symposium; 2010. 77-81. doi: 10.1109/TECHSYM.2010.5469196
14. Rheosys. Rheosys Merlyn II User Manual Design – Inovation, Volume 1. USA:
Plainsboro; 2008. 20-30.
15. Anah L, Astrini N. Sintesa dan karakterisasi hidrogel super absorben polimer (Sap)
berbasis selulosa menggunakan crosslinking agent water-soluble carbodiimide (Wsc).
Jurnal Selulosa. 2015; 5(1): 1 – 6. doi: 10.25269/jsel.v5i01.73
16. Yang T. Mechanical and swelling properties of hidrogels. Wiley Periodicals, Inc. 2012; 22.
17. Park YG, Lee DH, Park ES, Kim JY. Hidrogel and platelet-rich plasma combined treatment
to accelerate wound healing in a nude mouse model. Arch Plast Surg. 2017; 44(3): 194-201.
doi: 10.5999/aps.2017.44.3.194
18. Du L, Miao Y, Li X, Shi P, Hu Z. A Novel and convenient method for the preparation
and activation of PRP without any additives: temperature controlled PRP. Biomed
Research Interatioal. 2018; 1-12. doi: 10.1155/2018/1761865
19. Erizal Perkasa DP, Sulistioso GS, Sudirman, Juniarti Z, Hariyanti. sintesis dan karakterisasi biodegradabel hidrogel superabsorben poli (kalium akrilat)-gglukomanan dengan teknik iradiasi gamma. J Sains Materi Indonesia. 2017; 19(1): 32-38. doi: 10.17146/
jsmi.2017.19.1.4110
20. Peng L, Cheng Rong X, Wang Wei J, Xu, Dong X, Wang G. Preparation and evaluation
of porouus chitosan/collagen scaffolds for periodontal tissue engineering. J of Biactive and Compatible Polymer. 2006; 21: 207. doi: 10.1177/0883911506065100
21. Anward G, Hidayat Y, Rokhati N. Pengaruh konsentrasi serta penambahan gliserol
terhadap karakteristik film alginat dan kitosan. Jurnal Teknologi Kimia dan Industri. 2013;
2(3): 51-56.
22. Nurhayati, Tazwir, Murniyati. Ekstraksi dan karakterisasi kolagen larut asam dari kulit
ikan nila (Oreochomis Niloticus). Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 2013; 8(1): 85-92. doi: 10.15578/jpbkp.v8i1.56
23. Ariani MD, Matsuura A, Hirata I, Kubo T, Kato K, Akagawa Y. New development of carbonate apatite chitosan scaffold based on lyophilization technique for bone tissue engineering. Dent Mater J. 2013; 32(2): 317-325. doi: 10.4012/dmj.2012-257
24. Kumar P, Dehiya BS, Sindhu A. Comparative study of chitosan and chitosan-gelatin
scaffold for tissue engineering. International Nano Latters. 2017; 7: 285-290. doi: 10.1007/
s40089-017-0222-2
25. Arakawa C, Ronald, Tan S, Kim S, Wu B, Lee M. Photopolymerizable chitosan-collagen
hidrogels for bone tissue engineering. J Tissue Eng Regen Med. 2017; 11(1): 164174.
doi: 10.1002/term.1896
DOI: https://doi.org/10.22146/majkedgiind.44391
Article Metrics
Abstract views : 1854 | views : 1863Refbacks
- There are currently no refbacks.
Copyright (c) 2020 Majalah Kedokteran Gigi Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.