Effects of contact time of natural cu-zeolite on the growth of Streptococcus pyogenes and Pseudomonas aeruginosa

Erwid Fatchur Rahman; Bambang Dwirahardjo; Poerwati Soetji Rahajoe

doi:10.22146/majkedgiind.40566

Effects of contact time of natural cu-zeolite on the growth of Streptococcus pyogenes and Pseudomonas aeruginosa

https://doi.org/10.22146/majkedgiind.40566

Erwid Fatchur Rahman^(1*), Bambang Dwirahardjo⁽²⁾, Poerwati Soetji Rahajoe⁽³⁾

(1) Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Sultan Agung Islamic University, Semarang, Central Java Oral and Maxillofacial Surgery Specialty Program, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(2) Department of Oral and Maxilofacial Surgery, Faculty of Dentistry, Universitas Gadjah Mada
(3) Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Yogyakarta
(*) Corresponding Author

Abstract

Infection of a surgical wound due to bacteria is a major problem for surgical patients. Cu-zeolite is a material that can suppress bacterial growth with reversible cation characteristics and adsorption to be developed into non-toxic disinfectants for humans. Packaging uses filter paper to keep disinfectant solutions or instruments that will be sterilized clean. This study aimed to observe the effects of contact time of natural Cu-zeolite on the growth of Streptococcus pyogenes (S. pyogenes) and Pseudomonas aeruginosa (P. aeruginosa) bacteria. An experimental research was simple randomized design. Cu-zeolite 10 grams were packaged in Whatman no 42 paper bags measuring 5 x 5 cm², contacted for 15, 30 and 45 minutes in 99.5 ml of distilled water exposed to 0.5 x 108 CFU / ml of S. pyogenes and P. aeruginosa. Subsequently, bacterial growth was calculated using total plate count method. The average growth of S.pyogenes for 15, 30 and 45 minutes (1840 ± 571.236 CFU; 29 ± 16.33 CFU and 0 CFU) while P. aeruginosa was (2776 ± 725.277 CFU; 55 ± 23.214 CFU and 0 CFU) respectively. Based on the independent t-test on Cu-zeolite, the bacterial growth in the 15^th and 30^th minute contact between S. pyogenes and P. aeruginosa was significantly different (pth and 30^th minute contact.

Keywords

decreased bacterial growth; length of contact; natural packaged Cu-zeolite; P. aeruginosa; S. pyogenes

Full Text:

PDF

References

1. Kangau Z, Odhiambo E. Orthopedic and trauma nurses views on causes and prevention of surgical site infection SSI’s; School of Health and Social Studies; JAMK University of Applied Sciences; 2009.

2. Ghahramanloo A, Sadhegian A, Sohrabi K, Bidi A. A microbiologic investigation following the desinfection of irreversible hydrocolloid materials using the spray method. J Calif Dent Assoc. 2009; 37(7): 471-477.

3. Brett ME, Lackey L, McCreanor P. The influence of metalic biocid concentration
on the deactivation rate of Coliform in river water. ASEE Southeast Section Conference. American Society for Engineneering Education; 2014.

4. Orha C, Likarec M, Pode R, Burtica G. Studies regarding copper recovery from residual solution on natural zeolites. Chem. Bull. “Politechnica” Univ. (Timisoara). 2005; 50(64): 152-154.

5. Fatimah D. Modifikasi zeolit alam melalui penanaman inhibitor cu dengan metode batch sebagai bahan baku obat antiseptik. Bandung: Seminar Nasional Zeolit VI; 2009. 75.

6. Hobman JL, Crossman LC. Bacterial antimicrobial metal ion resistance. J Med
Microbiol. 2015; 64(5): 471-497. doi: 10.1099/jmm.0.023036-0

7. Rodriguez-Iznaga I, Petranovskii V, Rodriguez-Fuentes G, Bogdanchikova N,
Avalos M. Modification of mordernite and natural clinoptilolite by copper: role of drying temperature. Studies In Surface Science and Catalysis. 2001; 135: 212. doi: 10.1016/S0167-2991(01)81425-7

8. Borkow G, Gabbay J. Copper as a biocidal tool. Curr Med Chem. 2005; 12(18): 2163-21675. doi: 10.2174/0929867054637617

9. Tania L, Wijaya K, Trisunaryanti W. Sintesis Cu(II)/Silika dengan metode sol-gel sebagai antibakteri terhadap Escherichia coli dan Staphylococcus aureus. Berkala MIPA. 2014; 24(2); 122-135.

10. Niira R, Niira Y, Niira H, Yamamoto T, Uchida M. Antibiotic zeolite, United state patent Document no. 4938958; 1990.

11. Sandi DMN, Indarto, Bayuaji R, Ekaputri JJ. Study of using paper filter whatman no.42 for measuring water retention in cement paste. The Journal for Technology and Science. 2016; 27(3): 59-65. doi: 10.12962/j20882033.v27i3.1905

12. Nolte WA. Oral Microbiology, 4th ed. St. Louis: The CV Mosby Co; 1982. 68-75, 287, 302-5, 523-532.

13. Butcher W, Ulaeto. Contact inactivation of orthopoxviruses by household desinfectans. Journal of Applied Microbiology. 2005; 99(2): 279-284.
doi: 10.1111/j.1365-2672.2005.02601.x

14. Li B, Yu S, Hwang JY, Shi S. Antimicrobial vermiculit nanomaterial. J of Mineral and Material Characteristization and Engineering. 2002; 1(1): 61-67.

15. Rossainz-Castro LG, Gomez, De-La-Rosa, Olguin, MT, Alcantara-Diaz. Comparison between silver- and copper- modified zeoliterich tuffs as microbicide agents for Escherichia coli and Candida Albicans. 2016. Diunduh dari
http://www.ncbi.nlm.nih.govm/pubmed pada 15 Mei 2018.

16. Lin H, Li TX, Zong C, Wang S. The preparation and application of the bacteriostatic inorganic materials for environment decontamination. Beijing: Proceeding German-Chinese-Polish Symposium Environmental Engineering.
2003; 291(4): 579-590.

17. Demirci S, Ustaoglu Z, Yilmazer GA, Sahin F, Bac N. Antimicrobial properties of zeolite-X and zeolite-A ion-exchanged with silver,copper, and zink against a broad range of microorganism. Appl Biochem Biotechnol. 2014; 172(3): 1652-1662.
doi: 10.1007/sl2010-013-0647-7

18. Baharutan A, Rares FES, Soelingan S. Pola bakteri penyebab infeksi nosokimial pada ruang perawatan intensif anak di BLU RSUP Prof. Dr. R. D. Kandou Manado. Jurnal e-Biomedik. 2015; 3(1); 45-53. doi: 10.35790/ebm.3.1.2015.7417

19. Mazzola PG, Jozala AF, Novaes LCdL, Moriel P, Penna TCV. Minimal inhibitory concentration (MIC) determination of disinfectant and/or sterilizing agents. Braz. J. Pharm. Sci. 2009; 45(2); 241-248. doi: 10.1590/S1984-82502009000200008

20. Brooks GF, Butel JS, Morse SA. Mikrobiologi kedokteran jawetz. Melnick & Adelberg, Terj. Hartanto. Ed.23. Jakarta: EGC; 2008. 1-67, 635-58.

21. Suhendrayatna. Bioremoval logam berat dengan menggunakan mikroorganisme: suatu kajian kepustakaan. Seminar Bio Teknologi. Tokyo: Sinergi Forum-Institut of Technology; 2001.

22. Kawasaki K, Masahiro K, Fujitani T. Denture detergents containing antimicobial metal ions. United State Patent No 6468950 BI; 2002.

23. Hrenovic J, Milenkovic J, Ivankovic T, Rajic N. Antibacterial activity of heavy metalloaded natural zeolite. Journal of Hazardous Materials. 2012; 201-202: 260-264. doi: 10.1016/j.jhazmat.2011.11.079

DOI: https://doi.org/10.22146/majkedgiind.40566