Neutrophil count in the gingival wound healing process after apitoxin gel application (gingival wound healing model on Wistar rats)

Urfa Tabtila(1), Swastiana Eka Yunita(2), Muhammad Nabil Pratama(3), Juni Handajani(4*)

(1) Dentistry Study Program, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(2) Dentistry Study Program, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(3) Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta
(4) Department of Oral Biology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author


Periodontal disease has a high prevalence in Indonesia. Gingival disease is a periodontal disease if it is severe, requires surgical therapy called a gingivectomy. Surgical periodontal procedures lead to a gingival wound that is usually treated
using a periodontal pack. Periodontal pack has no curative property in general but assists in a wound healing process by protecting tissues. Neutrophils are inflammatory cells that act as a host’s first defense against infectious pathogens and they are important in wound healing. Wound healing can be accelerated by apitoxin gel from honeybee Apis mellifera species. The aim of this study was to determine the effectiveness of apitoxin gel on neutrophil count on gingival wound
healing process. Medical bee venom powder (Umea, Sweden) was mixed with double-distilled water to prepare a concentration of 0.01% bee venom. The solution was mixed with 10% propylene glycol, 0.01% methylparaben, and
3% CMC-sodium. Thirty six gingiva of 3 month-old male Wistar rats (weighing 200-250 gram) were injured using punch biopsy (3 mm in diameter). They were treated in 4 groups: apitoxin gel, apitoxin gel with a periodontal pack, NSAIDs
Flamar® gel with periodontal pack, and a periodontal pack. Three Wistar rats from each group were euthanized and their jaws were taken after 48 hours treatment to make histology slides with Hematoxylin Eosin (HE) staining. Neutrophil counts were observed. The data were analyzed using Anova test with 95% confidence level. The results showed there were significan defferebces among 4 groups (p<0.05). The highest neutrophil count on the 2nd day was found in the apitoxin gel induced the 2 day of gingival wound healing process.


apitoxin gel; Apis mellifera; gingival wound healing process; neutrophils

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1. Lestari DP, Wowor VN, Tambunan E. Hubungan tingkat pengetahuan kesehatan gigi dan mulut dengan status kesehatan jaringan periodontal pada penyandang diabetes melitus tipe 2 di RSUD Manembo-nembo Bitung. e-GIGI. 2016; 4(2): 188-195. doi: 10.35790/eg.4.2.2016.13926

2. Newman MG, Takei HH, Klokevold PR, Car-ranza FA, Newman and Carranza’s Clinical Periodontology, Missouri: Elsevier; 2016, 51-66, available at https://www-!/content/book/3s2.0B9780323523004000035?indexOverride=GLOBAL

3. Scheid RC, Weiss G. Woelfel’s Dental Anatomy, 8th ed. Philadelphia: Wolters Kluwer Lippincott Williams & Wilkins; 2012.

4. Polimeni G, Xiropaidis AV, Wikesjo UME. Biology and principles of periodontal wound healing/regeneration. Periodontol 2000. 2006; 41: 30-47. doi: 10.1111/j.1600-0757.2006.00157.x.

5. Low SB, Laser in Periodontal Surgery in Convistar RA, Principle and Practice of Laser in Dentistry, 2nd ed., Missouri: Elsevier; 2016, 51-66, available at!/content/book/3-s2.0-B9780323297622000048

6. Cohen ES. Atlas of cosmetic and reconstructive periodontal surgery, 3rd ed. Shelton: People’s Medical Publishing House; 2009.

7. Bird DL, Robinson DS. Modern dental assisting, 12th ed. Missouri: Elsevier; 2017.

8. Oliveira S, Rosowski EE, Huttenlocher A. Neutrophil migration in infection and wound repair: going forward in reverse. Nat Rev Immunol. 2016; 16(6): 378-391. doi: 10.1038/nri.2016.49

9. Paul, Willi and Sharma, Chandra P. Advances in wound healing materials: science and skin engineering. Shawbury: Smithers Rapra Technology; 2015.

10. Rosales C, Demaurex N, Lowell CA, UribeQuerol, E. Neutrophils: Their Role in Innate and Adaptive Immunity. J Immunol Res. 2016; 2017: 9748345. doi: 10.1155/2017/9748345

11. Hartini PS, Dewi N, Hayatie L. Ekstrak ikan haruan (Channa striata) menurunkan jumlah makrofag pada fase inflamasi proses penyembuhan luka. Dentofasial. 2015; 14(1): 6-10.

12. Leandro LF, Mendes CA, Casemiro LA, Vinholis AHC, Cunha WR, Almeida RD,
Martins CHG. Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens. An Acad Bras Cienc. 2015; 87(1): 147- 155. doi: 10.1590/0001-3765201520130511

13. Situmorang, Rospita OP, Hasanudin A. Panduan manual budidaya lebah madu.
Parapat: Balai Penelitian Kehutanan Aek Nauli; 2014.

14. Shiddiq A, Minarti S, Junus M. Quality of pollen grain and bee bread assessment of honey bees (apis mellifera) in the mango trees plantation area. Malang: Brawijaya University Press; 2013.

15.Ali MAAM. Studies on bee venom and its medical uses. International Journal of
Advancements in Research & Technology. 2012; 1(2): 69-83.

16.Han SM, Park KK, Nicholls YM, Macfarlane N, Duncan G. Effects of honeybee (Apis mellifera) venom on keratinocyte migration in vitro. Pharmacogn Mag. 2013; 9(35): 220-226. doi: 10.4103/0973-1296.113271

17. Moselhy WA, El-Didamony SE, Taher FA, Mohamed AF, Metwalley KM, Zayed AB. Evaluation of anticancer potentials of bee free venom and chitosan nano-conjugated one: in vitro study. IJSRM. 2017; 5(4): 5253-5262. doi: 10.18535/ijsrm/v5i4.01

18. Kusumastuti E, Handajani J, Susilowati H. Ekspresi COX-2 dan jumlah neutrofil fase inflamasi pada proses penyembuhan luka setelah pemberian sistemik ekstrak etanolik Rosela (Hibiscus sabdariffa) (studi in vivo pada Tikus Wistar). Majalah Kedokteran Gigi Indonesia. 2014; 21(1): 13-19.
doi: 10.22146/majkedgiind.8778

19. Palm NW, Medzhitov R. Role of the inflammasome in defense against venoms. Proc Natl Acad Sci U S A. 2013; 110(5): 1809-1814. doi: 10.1073/pnas.1221476110

20. Slatter, Douglas H. Textbook of small animal surgery. Philadelphia: Elsevier Science; 2003.

21. Golan, David E. Principles of pharmacology: the pathophysiologic basis of drug therapy. Philadelphia: Lippincott Williams & Wilkins; 2008.

22. Newman MG, Takei HH, Klokkevold PR, Carranza, Fermin A. Carranza’s Clinical
Periodontology, 12th ed. Canada: Elsevier Saunders; 2014.


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