Inhibition activity of Robusta coffee beans polyphenol extract on the production of TNF-α neutrophil cells

Tantin Ermawati(1*), Zahara Meilawaty(2), Happy Harmono(3)

(1) Department of Biomedical, Faculty of Dentistry, Universitas Jember, Jember, East Java
(2) Department of Biomedical, Faculty of Dentistry, Universitas Jember, Jember, East Java
(3) Department of Biomedical, Faculty of Dentistry, Universitas Jember, Jember, East Java
(*) Corresponding Author


Polyphenols are one of the active substances in the Robusta coffee beans with various benefits for humans’ health including anti-inflammation. neutrophil cell (polymorphonuclear PMN) plays a significant role as the primary immune response against foreign agent. Inflammatory response is characterized by the production of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α). Hence, the purpose of this study is to determine anti-inflammation capacity of Robusta coffee beans polyphenols extract on the TNF-α production in neutrophil cells. Neutrophil was derived from human peripheral venous blood by means of gradient density method. One hundred microliter of neutrophil  (1,5x103 cell) was incubated with 3.13%, 6.25%, 12.5%, 25% polyphenol extract and exposed with 100 μl of Lipopolysaccharides (LPS) 25 mg/ml. The production of TNF-α were determined by ELISA. Data were analyzed by one way Anova. Polyphenols of Robusta coffee bean extract was shown to inhibit TNF-α production in LPS-exposed neutrophil cells compared to controls. Whereas, LPS-exposed neutrophil increased TNF-α production. The most effective concentration to inhibit TNF-α production was 12.5%. It was concluded that polyphenols of Robusta coffee beans has anti-inflammatory properties as indicated by its ability to decrease TNF-α levels.


LPS; neutrophil; polyphenol; Robusta coffee beans; TNF-α

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