Effect of Porphyromonas gingivalis infection on leukocyte count in rat model of diabetes mellitus

https://doi.org/10.22146/majkedgiind.23067

Safira Niza Ulfita(1), Agustin Wulan Suci Dharmayanti(2*), Budi Yuwono(3)

(1) Dentistry Study Program, Faculty of Dentistry, Universitas Jember, East Java
(2) Biomedical Department, Faculty of Dentistry, Universitas Jember, East Java
(3) Oral Surgery Department, Faculty of Dentistry, Universitas Jember, East Java
(*) Corresponding Author

Abstract


Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia and become the third cause of death in Indonesia. The most common oral complications occured in DM is periodontitis. Porphyromonas gingivalis (Pg) is the main etiology of periodontitis, one of periodonthopathogen relate to systemic disease. However this theory still controversial, DM and periodontitis have the same pathogenesis, thus are cellular changes. Pg is predicted affect the total leukocytes count in DM. The aim of this study was to determine the effect of Pg infection to total leukocytes count in DM rat model. The rat model of DM was obtained by injection of streptozotocin with different dosage for 5 days sequentially then injected by Pg on distobuccal and distopalatal sulcus of the maxillary first molars for 19 days with interval 3 days. The total leukocytes were calculated manually by Neubauer improved cell counting chamber. The results demonstrated that rat model of DM which injected by Pg has the lowest total leukocytes count. Therefore, Pg infection decreased the total leukocytesin DM rat model.


Keywords


diabetes mellitus; leukocytes; periodontitis; Porphyromonas gingivalis

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References

Ngugi MP, Njagi JM, Kibiti CM, Ngeranwa JJN, Njagi ENM. Diagnosis of diabetes mellitus. Int J of Diabetes Research. 2012; 1(2): 24 – 27.

2. Kementerian Kesehatan RI. www.depkes.go.id. 2006; [diakses pada 3 Agustus 2016].

3. Graves DT, Kayal RA. Diabetic complication and dysregulated innate immunity. Front Biosci National Institutes of Health. 2011; 13: 1227 – 1239.

4. Stanko P, Holla LI. Bidirectional association between diabetes mellitus and inflammatory periodontal disease: a review. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014; 158(1): 35 – 38.

5. Preshaw PM, Alba AL, Herrera D, Jepsen S, Konstantinidis A, Makrilakis K, Taylor R. Periodontitis and diabetes: a two-way relationship. Diabetologia. 2011; 55: 21 – 31.

6. Deshpande K, Jain A, Sharma RK, Prashar S, Jain R. Diabetes and periodontitis. J Indian Soc Periodontol. 2010; 14(4): 207 – 212.

7. Vozarova, B, Weyer C, Lindsay RS, Pratley RE, Bogardus C, Tataranni C. High white blood cell count is associated with a worsening of insulin sensitivity and predicts the development of type 2 diabetes. Diabetes Journal. 2002; 51: 455 – 461.

8. Mealey BL. Periodontal disease and diabetes a two-way street. JADA. 2006; 139(3): 252.

9. Igari K, Kudo T, Toyofuku T, Inoue Y, Iwai T. Association between periodontitis and development of systemic disease [Internet]. Oral Biology and Dentistry; 2014 [diakses pada 3 Agustus 2016]. Tersedia dari http://www.hoajonline.com/oralbioldent/2053-5775/2/4.

10. Grenier D, Tanabe S. PG gingipains trigger a proinflammatory response in human monocyte-derived macrophages through the p38α mitogen-activated protein kinase signal transduction pathway [Internet].2010[diakses pada 2 Agustus 2016]. Tersedia dari www.mdpi.com/journal/toxin.

11. How KY, Song KP, Chan KG. Porphyromonas gingivalis: an overview of periodontopathic pathogen below the gum line. Front in Micro. 2016; 7: 53.

12. Mysak J, Podzimek T, Sommerova P, Lyuya-Mi Y, Bartova J, Janatova T, Prochazkova J, Duskova J. Review article Porphyromonas gingivalis: major periodontopathic pathogen view. Journal of Immunology Research: Hindawi Publishing Corperation. 2014; 2014: 1 – 8.

13. Carvalho-Filho PC, Gomes-Filho IS, Meyer R, Olczak T, Xavier MT, Trindade SC. Role of PGHmuY in immunopathogenesis of chronic periodontitis. Journal Mediators of Inflammation. 2016; 2016(1): 1 – 9.

14. Laheij AMGA, Loveren CV, Deng D, Soetde JJ. The impact of virulence factors of PGon wound healing in vitro. Journal of Oral Microbiology. 2015; 7(10): 1 – 8.

15. Ates AH, Canpolat U, Yorgun H, Kaya EB, Sunman H, Demiri E, Taher A, Hazirolan T, Aytemir K, Tokgözoglu L, Kabakçi G, Oto A. Total white blood cell count is associated with the presence, severity and extent of coronary atherosclerosis detected by dual-source multislice computed tomographic coronary angiography. Cardiol J. 2011; 18(4): 371 – 377.

16. Leng SX, Xue Q, Huang Y, Ferrucci L, Fried LP, Walston JD. Baseline total and specific differential white blood cell counts and 5 year all causemortality in community-dwelling older women. Exp. Gerontol. 2005; 40: 982 – 987.

17. Riley LK, Rupert J. Evaluation of patients with leukocytosis. American Family Physician. 2015; 92(11): 1004 – 1011.

18. Al-Rasheed A. Elevation of white blood cells and platelet counts in patients having chronic periodontitis. Saudi Dental Journal: Elsevier. 2012; 24(1): 17 – 21.

19. Notoatmodjo S. 2010. Metodologi Penelitian Kesehatan. Jakarta: Rineka Pustaka: 33 – 34.

20. Kusumawardani B. Dampak infeksi PG pada jaringan periodontal maternal terhadap pertumbuhan janin: analisis pengaruh ekspresi TLR-2, TLR-4, TNF, IL-10 dan caspase-3 pada plasenta tikus terhadap penurunan berat plasenta, berat janin, dan panjang janin. Disertasi. Yogyakarta: Universitas Gadjah Mada; 2012.

21. Hikmah N, Shita ADP, Maulana H. Rat diabetic blood glucose level profile with stratified dosage streptozotocin (SD-STZ) and multi low dosage streptozotocin (MLD-STZ) induction methods. The Journal of Tropical Life Science. 2015; 5(1): 30 – 34.

22. Chakravarti, Bhattacharya. A Handbook of Clinical Pathology. Academic Publisher; 2005:   5 – 7.

23. Borné Y, Smith JG, Nilsson PM, Melander O, Hedblad B, Engström G. Total and differential leukocyte counts in relation to incidence of diabetes mellitus: a prospective population-based cohort study. PLoS ONE Journal. 2016; 1 – 13.

24. Jiang H, Yan WH, Li CJ, Wang AP, Dou JT, Mu YM. Elevated white blood cell count is associated with higher risk of glucose metabolism disorders in middle-aged and elderly chinese people. International Journal of environment Reseacrh Public Health. 2014; 11(5): 5497 – 5509.

25. Shim WS, Kim HJ, Kang ES, Ahn CW, Lim SK, Lee HC, Cha BS. The association of total and differential white blood cell count with metabolic syndrome in type 2 diabetic patients. Diabetes Research and Clinical Practice. 2006; 73: 284 – 291.

26. Hirasawa H, Shigeto Oda, Masataka Nakamura. Blood glucose control in patients with severe sepsis and septic shock. World J Gastroenterol. 2009; 15(33): 4132 – 4136.

27. Koh GC, Peacock SJ, van der Poll T, Wiersinga WJ. The impact of diabetes on the pathogenesis of sepsis. Eur J Clin Microbiol Infect Dis. 2012; 31: 379 – 388.

28. Yun PL, Decarlo AA, Hunter N. Gingipains of Porphyromonas gingivalis modulate leukocyte adhesion molecule expression induced in human endothelial cells by Ligation of CD99. Infection and Immunity. 2006; 74(3): 1661 – 1672.

29. Gan YH. Host susceptibility factors to bacterial infections in type 2 diabetes. PLOS Pathogen Journal. 2013; 9(12): 1 – 3.

30. Syaify A. Pengaruh level HBA1C terhadap fungsi fagositosis neutrofil (PMN) pada penderita periodontitis diabetika. Majalah Kedokteran Gigi Indonesia. 2012; 19(2): 93 – 97.

31. Liu H, Cheng Z, Song W, Wu We, Zhou Z. Immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella pneumonia bacteremia. PLoS ONE Journal. 2014; 9(10): 1 – 5.

32. Iskander KN, Osuchowski MF, Stearns-Kurosawa DJ, Kurosawa S, Stepien D, Valentine C, Remick DG. Sepsis: multiple abnormalities, heterogenous responses, and evolving understanding. American Physiological Society. 2013; 93(3): 1247 – 1288.

33. Laszlo I, Trasy D, Molnar Z, Fazakas J. Review article sepsis: from pathophysiology to individualized patient care. Journal of Immunology Research. 2015; 2015: 1 – 14.

34. Li X, Kolltveit KM, Tronstad L, Olsen I. Systemic diseases caused by oral infection. Clin. Microbiol. Rev. 2000; 13(4): 547 – 558.

35. Abramson N, Melton B. Leukocytosis: basics of clinical assessment. American Family Physician. 2000; 62(9): 2053 – 2060.

36. Heidt T, Sager HB, Courties G, Dutta P, Iwamoto Y, Zaltsman A, Muhlen CVZ, Bode C, Fricchione GL, Denninger J, Lin CP, Vinegoni C, Libby P, Swirski FK, Weissleder R, Nahrendorf M. Chronic variable stress activates hematopoietic stem cells.Nature Medicine. 2014; 20: 754 – 758.



DOI: https://doi.org/10.22146/majkedgiind.23067

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