Gambaran histopatologi penyembuhkan luka pencabutan gigi pada makrofag dan neovaskular dengan pemberian getah batang pisang ambon

Hendrik Setia Budi; Pratiwi Soesilowati; Zhafirah Imanina

doi:10.22146/majkedgiind.17454

Gambaran histopatologi penyembuhkan luka pencabutan gigi pada makrofag dan neovaskular dengan pemberian getah batang pisang ambon

https://doi.org/10.22146/majkedgiind.17454

Hendrik Setia Budi^(1*), Pratiwi Soesilowati⁽²⁾, Zhafirah Imanina⁽³⁾

(1) Departemen Biologi Mulut, Universitas Airlangga, Jawa Timur
(2) Departemen Biologi Oral, Fakultas Kedokteran Gigi, Universitas Airlangga, Surabaya, Jawa Timur
(3) Departemen Biologi Oral, Fakultas Kedokteran Gigi, Universitas Airlangga, Surabaya, Jawa Timur
(*) Corresponding Author

Abstract

Tooth extraction healing on macrophages and neovascular histopathology induced by ambonese banana stem sap gel. Blood clotting is very important in the early wound healing process. Blood clots will soon be filled with a connective tissue due to the induction of growth factors as granulation tissue. The expression of platelet derived growth factors (PDGF)-BB using the Ambonese banana stem sap gel (Musa paradisiaca var. sapientum) can increase the proliferation of fibroblasts. Macrophages and neovascular support in the early phase of healing. The purpose of this research was to demonstrate that Ambonese banana stem sap gel (GEGPA) can improve the macrophages and neovascular in the socket wound healing. Thirty six wistar rats were used and divided into 3 groups. The mandibular left incisor was extracted. The first group as control was given HPMC 4% gel on socket, the second group was given GEGPA 60%, and third group was given gelatin sponge. Rats were sacrificed on day 3 and 5 for histopathology examination of neovaskular and macrophages on the socket. The results showed that there was significantly different in the control group on a number of neovascular and macrophages in socket on day 3 (p<0.05). No significant difference on day 5 in all groups of neovascular and macrophages number (p>0.05). It was concluded that the use of GEGPA 60% accelerated
the wound healing of tooth extraction socket by means of macrophages and neovascular increasing.

ABSTRAK

Pembekuan darah sangat penting pada proses awal penyembuhan luka. Bekuan darah akan segera diisi oleh jaringan granulasi yang berasal dari induksi faktor pertumbuhan. Ekspresi platelet derived growth factors (PDGF)-BB yang diinduksi oleh pemberian gel ekstrak getah batang pisang ambon (Musa paradisiaca var. sapientum) dapat menunjukkan terjadinya peningkatan proliferasi fibroblas. Makrofag dan neovaskular sangat menunjang fase awal penyembuhan. Tujuan penelitian ini untuk membuktikan bahwa pemberian gel ekstrak getah batang pisang ambon (GEGPA) dapat meningkatkan jumlah makrofag dan neovaskular pada penyembuhan luka soket. Tikus strain Wistar sebanyak 36 ekor digunakan pada penelitian ini yang terbagi menjadi 3 kelompok. Semua tikus dilakukan pencabutan gigi pada insisive kiri
mandibula. Kelompok pertama sebagai kontrol, pada soket diberi gel hidroksipropil metil selulosa (HPMC) 4%, kelompok kedua diberi GEGPA 60%, dan kelompok ketiga diberi gelatin sponge. Tikus dikorbankan pada hari ke-3 dan 5 untuk pemeriksaan histopatologi neovaskular dan makrofag pada soket. Hasil penelitian menunjukkan terdapat perbedaan yang bermakna terhadap kelompok kontrol pada jumlah neovaskular dan makrofag soket pada hari ke-3 (p<0,05). Tidak ditemukan perbedaan yang bermakna pada jumlah neovaskular dan makrofag hari ke-5 (p>0,05). Disimpulkan bahwa pemberian GEGPA 60% dapat mempercepat penyembukan luka pencabutan gigi melalui peningkatan jumlah makrofag dan neovaskular.

Keywords

ambonese banana stem sap; histopathology; macrophage; neovascular; socket

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References

1. McLennan SV, Bonner J, Milne. Matrix metalloproteinases and their roles in poor
wound healing in diabetes. Wound Prac Res. 2008; 16(3): 116 – 121.

2. Velnar T, Bailey T, Smrkolj V. The wound healing process: an overview of the cellular and molecular mechanisms. J Int Med Res. 2009; 37(5): 1531 – 1534.

3. Falanga V. Wound healing and its impairment in the diabetic foot. Lancet 2005; 366: 1736 – 1743.

4. Brancato SK, Albina JE. Wound macrophages as key regulators of repair origin, phenotype, and function. Am J Pathol. 2011; 178(1): 19 – 25.

5. Koh TJ, DiPietro LA. Inflammation and wound healing: the role of the macrophage. Expert Rev Mol Med. 2011; 11(13): e23. doi: 10.1017/S1462399411001943.

6. Reinke JM, Sorg H. Wound repair and regeneration. Eur Surg Res. 2012; 49: 35 – 43.

7. OGTR. The biologi of musa. Document Prepared by the Office of the Gene Technolgy
Regulator. Vol 1. Canberra. Australia. 2008 (cited2008 November 11). Available online on
http://www.ogtr.gov.au.

8.Coopoosamy RM. In-vitro studies on lectin derivatives of aloe excelsa (Berger). J Med Plants Res. 2010; 4(17): 1738 – 1742.

9. Budi HS, Kriswandini IL, Sudjarwo SA. Ambonese banana stem sap (Musa paradisiaca var. sapientum) effect on PDGFBB expressions and fibroblast proliferation in socket wound healing. Int J ChemTech. 2016; 9(12): 87 – 95.

10. Budi HS, Arundina I, Indrawati R, Mahardikasari L. Uji toksisitas akut ekstrak batang pisang ambon (Musa paradisiacavar sapientum) terhadap hati mencit (Mus musculus) dengan parameter LD (Acute toxicity test of ambonese banana (Musa paradisiaca var sapientum) stem extract in liver of mice (Mus musculus) with LD 50 parameters 50. Journal of Dentomaxillofacial Science. 2014; 13(2): 86 – 90. DOI:10.15562/jdmfs.v13i2.394.

11. Swanson MD. Molecular engineering of a banana lectin that inhibits hiv-1 replication.
The University of Michigan, Dissertation; 2010. 16.

12. Iqbal RZ, Song-hua H, Chen-wen X, Arijo AG. Adjuvant effects of saponins on animal
immune responses. J Zhejiang Univ Sci. 2007; 8(3): 153 – 161.

13. Kaigler D, Silva EA, Mooney DJ. Guided bone regeneration (GBR) utilizing injectable
vascular endothelial growth factor (VEGF) delivery gel. J Periodontol. 2012. DOI: 10.1902/jop.2012.110684.

14. Basi DL, Hughes PJ, ThumbigereMath V, Sabino M, Lunos SA, Jensen E, Gopalakrishnan R. Matrix metalloproteinase-9 expression in alveolar socket of zoledronic acid-treated rats. J. Oral Maxillofac Surg. 2011; 69(11): 2698 – 2707.

15. Simion M, Rocchietta I, Kim D, Nevins M, Fiorellini J. Vertical ridge augmentation by means of deproteinized bovine bone block and recombinant human platelet-derived growth factor-BB: a histologic study in a dog model. Int J Periodontics Restorative Dent. 2006; 26: 415 – 423.

16. Bum SK, Young CN, Young WJ. Comparison of the wound healing effect of cellulose and
gelatin: An in vivo study. Arch Plast Surg. 2012; 39(4): 317 – 321.

17. Garrant PR. Oral cells and tissues. New York: Quintessence Publishing, Stony Brook; 2003.
195 – 219.

18. Zhang YD, Chen Z, Song YQ, Liu C, Chen YP. Making a tooth: Growth factors, transcription
factors, and stem cells. Cell Research. 2005; 15(5): 301 – 316.

19. Fiedler J, Roderer G, Gunther KP, Brenner RE. BMP-2, BMP-4 and PDGF-BB stimulate chemotactic migration of primary human mesenchymal progenitor cells. Journal of Cell Biochemistry. 2002; 87: 305 – 312.

20. Schwarz F, Ferrari D, Sager M, Herten M, Hartig B, Becker J. Guided bone regeneration
using rhGDF-5- and rhBMP-2-coated natural bone mineral in rat calvarial defects. Clin Oral Implants Res. 2009; 20: 1219 – 1230.

21. Stephan EB, Renjen R, Lynch SE, Dziak R. Platelet-derived growth factor enhancement
of a mineral-collagen bone substitute. J Periodontol. 2000; 71: 1887 – 1892.

22. Nevins M, Giannobile WV, McGuire MK, Kao RT, Mellonig JT, Hinrichs JE, McAllister BS,
Murphy KS, McClain PK. Platelet-derived growth factor stimulates bone fill and rate
of attachment level gain: results of a large multicenter randomized controlled trial. J
Periodontol. 2005; 76: 2205 – 2215.

23. Camelo M, Nevins ML, Schenk RK, Lynch SE, Nevins M. Periodontal regeneration
in human Class II furcations using purified recombinant human platelet-derived growth
factor-BB (rhPDGF-BB) with bone allograft. Int J Periodontics Restorative Dent. 2003; 23:
213 – 225.

DOI: https://doi.org/10.22146/majkedgiind.17454