Efek Antimikroba Pasta Gigi Kandungan Ekstrak Daun Teh 2% (Camellia sinensis) terhadap A. Actinomycetemcomitans

https://doi.org/10.22146/majkedgiind.15592

Juni Handajani(1*)

(1) Bagian Biologi Mulut, Fakultas Kedoteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


Latar Belakang: Kandungan polifenol (catechin) dalam daun teh diketahui memiliki efek antimikroba terhadap beberapa bakteri. Derajat fermentasi terhadap daun teh akan mempengaruhi daya antimikrobanya. Pasta gigi dengan kandungan ekstrak daun teh segar 2% (Camellia sinensis) diharapkan memiliki efek antimikroba. Tujuan: pengetahuan ini bertujuan untuk mengetahui daya antimikroba pasta gigi kandungan ekstrak teh segar 2% terhadap bekteri A. actinomycetemcomitans. Metode Penelitian: bakteri A. actinomycetemcomitans diperololeh dari laboratorium MikrobiologiFakultas Kedokteran Hewan Universitas Gadjah Mada Yogyakarta. Pengenceran pasta gigi dengan kandungan teh 2% diencerkan menjadi 0,875%; 1,75%; 2,5%; 5%; 10%; dan 100% (kontrol positif). Metode difusi dilakukan pada 15 petri dengan cara masing-masing konsentrasi diambil 100 ul lalu diteteskan dalam setiap sumuran pada media BHI agar yang telah mengandung A. Actinomycetemcomitans kemudian petri dieramkan dalam inkubator selama 24 jam pada suhu 370 C. Pengukuran diameter zone hambatan disekitar sumuran menggunakan jangka sorong, dibantu dngan mikroskop, selanjutnya data dianalisis menggunakan AVANA dan LSD. Hasil menunjukkan daya anti bakteri pasta gigi kandungan ekstrak teh 2% terhadap bakteri A. Actinomycetemcomitans dimulai pada konsentrasi 5% dan terdapat perbedaan yang bermakna (p<0,01) pengaruh konsentrasi terhadap zone hambatan yang dihasilkan. Disimpulkan peningkatan konsentrasi pasta gigikandungan ekstrak teh akan meningkatkan daya antimikroba terhadap bakteri A. Actinomycetemcomitans. 

 

Background: Polyphenols (catechins) in tea leaves are known to have antimicrobial effects against some bacteria. Degree of fermentation tea leaves will affect the antimicrobial. Toothpaste containing 2% tea leaves ectract (Camellia sinensis) is expected to have antimicrobial effects. Aim: this study was aimed to determine the antimicrobial effect of tootthpastes contain 2% tea extract on the bacteria A. Actinomycetemcomitans. Method: Aggregatibacter actinomycetemcomitans bacteria from Microbiology Laboratory, Faculty of Veterinary Medicine, Gadjah Mada University Yogyakarta. Tea extract-containing dentrifice was dilluted into 0,875%; 1,75%; 2,5%; 5%; 10%; dan 100% (as positive control). Diffusion method was used on 15 disks. Every concentration of tea extract-containing dentrifice was placed in bottomless cylinder in BHI agar disks that have been heavily seeded with A. Actinomycetemcomitans , then incubated for 24 hours at 370. The diameter of the clear zone of inhibition was measured using sliding caliper and microscope then data were analyzed using AVANA and LSD. The result showed that the antimicrobial effect of  tea extract-containing dentrifice was started at 5% and there were significantly difference (p<0,01) effect concentration of  tea extract-containing dentrifice on zone of inhibition. In conclusion, increasing concentration of  tea extract-containing dentrifice could increase the antimicrobial effect against A. Actinomycetemcomitans. 


Keywords


daya antimikroba; pasta gigi; ekstrak daun teh; Aggregatibacter actinomycetemcomitans; antimicrobial effect; tea extract-containing dentrifice; aggregatibacter actinomycetemcomitans

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DOI: https://doi.org/10.22146/majkedgiind.15592

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