* Corresponding Author
The impact of varying concentrations of hyaluronic acid gel on the enhancement of VEGF and angiogenesis expression
https://doi.org/10.22146/majkedgiind.104632 Malianawati Fauzia(1*), Ega Lucida Chandra Kumala(2), Maulida Aufani(3), Addini Zoya Syalisha(4), Adellia Wahyu(5), Muhammad Maulana Zikrullah Mukhlis(6)
(1) Department of Periodontics, Faculty of Dentistry, Universitas Brawijaya, Malang, East Java, Indonesia
(2) Department of Periodontics, Faculty of Dentistry, Universitas Brawijaya, Malang, East Java, Indonesia
(3) Bachelor Degree of Dentistry, Faculty of Dentistry, Universitas Brawijaya, Malang, East Java, Indonesia
(4) Bachelor Degree of Dentistry, Faculty of Dentistry, Universitas Brawijaya, Malang, East Java, Indonesia
(5) Bachelor Degree of Dentistry, Faculty of Dentistry, Universitas Brawijaya, Malang, East Java, Indonesia
(6) Bachelor Degree of Dentistry, Faculty of Dentistry, Universitas Brawijaya, Malang, East Java, Indonesia
(*) Corresponding Author
Abstract
Periodontal dressings can contribute to plaque accumulation, which in turn may cause inflammation, irritation of healing tissues, bacteremia, and difficulties in eating. Advances in knowledge and technology have improved the materials used in these dressings, aiming to enhance effectiveness and minimize complications. High-molecular-weight hyaluronic acid (HMW-HA) is a promising dressing material because of its anti-inflammatory properties and its ability to promote epithelial regeneration. This study aimed to determine whether different concentrations of HMW-HA gel influence vascular endothelial growth factor (VEGF) expression and angiogenesis during gingival wound healing in white rats (Rattus norvegicus) of the Wistar strain. A total of 42 rats were divided into seven groups: a negative control group (K1), a positive control group (K2) treated with 0.2% Gengigel®, and five treatment groups (P1–P5) receiving HMW-HA gel at concentrations of 0.2%, 0.4%, 0.6%, 0.8%, and 1%, respectively. Standardized 2 mm wounds were created with a punch biopsy on the lower anterior jaw of each rat. Treatments were administered twice daily for seven days. VEGF expression and angiogenesis were evaluated using immunohistochemical and hematoxylin–eosin staining. The group treated with 1% HA gel (P5) showed the highest average VEGF expression and angiogenesis compared with the negative control and lower-concentration treatment groups. These findings suggest that topical application of HMW-HA gel may enhance gingival wound healing in periodontal surgery by promoting angiogenesis and increasing VEGF expression.
Keywords
Full Text:
4. Malianawati FauziaReferences
1. Nascimento GG, Alves-Costa S, Romandini M. Burden of severe periodontitis and
edentulism in 2021, with projections up to 2050: the global burden of disease 2021
study. J Periodontal Res. 2024; 59(5): 823-867. doi: 10.1111/jre.13337
2. Hijryana M, MacDougall M, Ariani N, Saksono P, Kusdhany LS, Walls AWG. Periodontal disease and oral health–related quality of life in the older population in Indonesia. JDR Clin
Trans Res. 2022; 7(3): 277–288. doi: 10.1177/23800844211021391
3. Suryono S, Yassa RM, Saputro LT, Walianto AP, Seno Aji NRA. Antibacterial potential from periodontal dressing raw propolis Trigonaitama bee based against Porphyromonas gingivalis: experimental research. Jurnal Kedokteran Gigi Universitas Padjadjaran. 2023; 35(1): 6-13. doi: 10.24198/jkg.v35i1.46229
4. Kathariya R, Jain H, Jadhav T. To pack or not to pack: the current status of periodontal
dressings. J Appl Biomater Funct Mater. 2015; 13(2): 73–86. doi: 10.5301/jabfm.5000215
5. Dias Moreira T, Martins V, da Silva Júnior A, Sayer C, Araújo P, Immich A. New insights
into biomaterials for wound dressings and care: Challenges and trends. Prog Org Coat.
2024; 187: 108118. doi: 10.1016/j.porgcoat.2023.108118
6. Asparuhova MB, Kiryak D, Eliezer M, Mihov D, Sculean A. Activity of two hyaluronan
preparations on primary human oral fibroblasts. J Periodontal Res. 2019; 54(1): 33–45. doi: 10.1111/jre.12602
7. Ramadhany EP, Ambarawati IGAD, Musyaffa MR. Effect of 4% and 15% moringa leaf extract
gel on gingival wound healing in rats. Majalah Kedokteran Gigi Indonesia. 2023; 8(3): 192-
199. doi: 10.22146/majkedgiind.76416
8. Doğan SSA, Karakan NC, Doğan Ö. Effects of topically administered 0.6% hyaluronic acid on the healing of labial frenectomy in conventional and 940-nm indium gallium arsenide phosphide (InGaAsP) diode laser techniques in pediatric patients: a randomized, placebo-controlled clinical study. Lasers Med Sci. 2024; 39(1): 48. doi: 10.1007/s10103-024-03983-7
9. Ripollés de Ramón Calle Platerías J. Pilot clinical study of the efficacy of a hyaluronic acid (1%) and chlorhexidine (0.20%) gel in post-extraction dentistry. Cientifica Dental. 2021; 17: 4-11.
10. Muche-Borowski C, Selbmann H, Nothacker M, Müller W, Kopp I. AWMF guidance manual
and rules for guideline development. Version 2.1; 2023. 91
11. Rujirachotiwat A, Suttamanatwong S. Curcumin upregulates transforming growth factor-β1, its receptors, and vascular endothelial growth factor expressions in an in vitro human gingival fibroblast wound healing model. BMC Oral Health. 2021; 21(1): 535. doi: 10.1186/s12903-021-01890-9
12. De la Riva B, Sánchez E, Hernández A, Reyes R, Tamimi F, López-Cabarcos E, et al. Local controlled release of VEGF and PDGFfrom a combined brushite–chitosan system enhances bone regeneration. J Control Release. 2010; 143(1): 45–52.
doi: 10.1016/j.jconrel.2009.11.026
13. Leśków N, Karp Z, Banaszewski M, Popielska K, Grześkowiak M, Mikołajski J, et al.
Characteristics and cellular mechanism of the wound healing process in the oral mucosa.
Medical Journal of Cell Biology. 2023; 11(1): 1–12. doi: 10.2478/acb-2023-0001
14. Ayu Wulan Sari D, Cahyati M. Pengaruh gel ekstrak daun kersen (Muntingia calabura L.)
terhadap ekspresi VEGF pada penyembuhan luka pasca pencabutan gigi tikus (Rattus norvegicus). E-Prodenta Journal of Dentistry. 2024; 8(1): 123–132.
15. Valachová K, Šoltés L. Hyaluronan as a prominent biomolecule with numerous
applications in medicine. Int J Mol Sci. 2021; 22(13): 7077. doi: 10.3390/ijms22137077
16. Hong L, Shen M, Fang J, Wang Y, Bao Z, Bu S, et al. Hyaluronic acid (HA)-based hydrogels
for full-thickness wound repairing and skin regeneration. J Mater Sci Mater Med. 2018;
29(9): 150. doi: 10.1007/s10856-018-6158-x
17. Bonatto M da S, Feltran G da S, Barbosa TP, Pereira DA, Santos S de S, Mendes PGJ, et
al. Green tea and hyaluronic acid gel enhance fibroblast activation and improves the gingival
healing post-third molar extraction. Sci Rep. 2024; 14(1): 7124. doi: 10.1038/s41598-024-57821-5
18. Canciani E, Sirello R, Pellegrini G, Henin D, Perrotta M, Toma M, et al. Effects of vitamin
and amino acid-enriched hyaluronic acid gel on the healing of oral mucosa: in vivo and in
vitro study. Medicina (B Aires). 2021; 57(3): 285. doi: 10.3390/medicina57030285
19. Yang H, Song L, Zou Y, Sun D, Wang L, Yu Z, et al. Role of hyaluronic acids and potential as regenerative biomaterials in wound healing. ACS Appl Bio Mater. 2021; 4(1): 311–324. doi: 10.1021/acsabm.0c01364
20. Hwang HS, Lee CS. Recent progress in hyaluronic-acid-based hydrogels for bone tissue engineering. Gels. 2023; 9(7): 588. doi: 10.3390/gels9070588
21. Gupta S. Evaluation of gengigel® application in the management of furcation with coronally
advanced flap through surgical re-entry- a split mouth clinical study. J Clin Diagn Res.
2017; 11(1): ZC27-ZC32. doi: 10.7860/JCDR/2017/21938.9169
22. Al-Saadi TS, Al-Quisi AF. The influence of the hyaluronic acid gel on the postoperative
sequelae following surgical removal of the impacted mandibular third molar in comparison with the A-PRF: a randomized controlled trial. Int J Biomater. 2023; 2023: 1–7. doi: 10.1155/2023/1883460
23. Kim YJ, Carvalho FC, Souza JA, Gonçalves PC, Nogueira AV, Spolidório LC, Roque-Barreira MC, Cirelli JA. Topical application of the lectin Artin M accelerates wound healing in rat oral mucosa by enhancing TGF-β and VEGF production. Wound Repair Regen. 2013; 21(3): 456-463.
doi: 10.1111/wrr.12041.
24. Snetkov P, Zakharova K, Morozkina S, Olekhnovich R, Uspenskaya M. Hyaluronic acid: the influence of molecular weight on structural, physical, physico-chemical, and degradable properties of biopolymer. Polymers (Basel). 2020; 12(8): 1800. doi: 10.3390/polym12081800
25. Jain Y. Clinical evaluation of 0.2% hyaluronic acid containing gel in the treatment of
gingivitis. Medical Journal of Dr D.Y. Patil University. 2013; 6(4): 416-420. doi: 10.4103/0975-2870.118296
26. Kawano Y, Patrulea V, Sublet E, Borchard G, Iyoda T, Kageyama R, et al. Wound healing
promotion by hyaluronic acid: effect of molecular weight on gene expression and in
vivo wound closure. Pharmaceuticals. 2021; 14(4): 301. doi: 10.3390/ph14040301
DOI: https://doi.org/10.22146/majkedgiind.104632
Article Metrics
Abstract views : 1535
| 
views : 337
Refbacks
- There are currently no refbacks.
Copyright (c) 2025 Majalah Kedokteran Gigi Indonesia
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.