Penurunan Kadar IL-1β Makrofag Terpapar Agregat Bakteri Actinomycetemcomitans setelah Pemberian Minyak Atsiri Temu Putih

https://doi.org/10.22146/majkedgiind.10158

Juni Handajani(1*), Siti Fatimah(2), Ristini Asih(3), Antinah Latif(4)

(1) Bagian Biologi Mulut, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Program Studi Ilmu Keperawatan Gigi, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Program Studi Ilmu Keperawatan Gigi, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(4) Program Studi Ilmu Keperawatan Gigi, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


IL-1β level of macrophage exposed to A. actinomycetemomitans decreases after administration Curcuma Zedoaria volatile oil. Activation of interleukin-1β (IL-1β) is a key regulator of the inflammatory response. Macrophage is a phagocytic mononuclear cell that plays an important role in innate and adaptive immune response. The cytokine secreted by macrophages in response to pathogen are IL-1, IL-6, IL-12, TNF-α and chemokine. Curcuma zedoaria volatile oil may have anti inflammation effect. The aim of this study was to investigate IL-1β level of macrophage exposed to A. actinomycetemcomitans after administration Curcuma zedoaria volatile oil. The subjects were 10 male Wistar rats, which divided into two groups (treatment and control), each group 5 rats. In the treatment group, 30,6 μl/ml Curcuma zedoaria volatile oil was administered per oral for 14 days and the control group used aquabidest. In the 7th days, 100 μl A. actinomycetemcomitans in CMC 2% were applied on the anterior gingival mandible for 7 days. Rats were anesthetized in the 15th days then macrophage was collected from peritoneal. Interleukin-1β level of macrophage was measured using ELISA kit (R&D Systems, USA). Data were analyzed using t-test. The result showed IL-1β level decreased after treatment. The comparison between treatment and control was significant difference (p<0.05). It can be concluded that Curcuma zedoaria volatile oil may have anti inflammation effect through reducing the IL-1β level of macrophage.


Keywords


Curcuma zedoaria volatile oil; macrophage; IL-1β level; Curcuma zedoaria volatile oil; macrophage; IL-1β level

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DOI: https://doi.org/10.22146/majkedgiind.10158

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