Jurnal Sain Veteriner
type: channel
description: Jurnal Sain Veteriner (JSV), with registered number ISSN 0126-0421 (print), ISSN 2407-3733 (online) is a national scientific journal published by Faculty of Veterinary Medicine, Universitas Gadjah Mada and Indonesian Veterinary Medical Association (PDHI) with Cooperation Agreement No. 005/PBPDHI.KU/PK/III/2024 and No. 882/UN1/FKH/TU/HK.4/2024. The journal is accredited by Director General of Higher Education, Research, and Technology Number 10/C/C3/DT.05.00/2025 dated March 21, 2025 concerning the Accreditation Ranking of Scientific Journals for Period I of 2025 that JSV occupies the Sinta 2 Ranking (S2). Publication in JSV including original articles, reviews and case reports of a high scientific and ethical standard in all aspects of veterinary and biomedical research. Since 2020, JSV has been published 3 times a year, in April, August and December. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. JSV encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health.
publisher: Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI
language: en-US
publication name: Jurnal Sain Veteriner
issn: 2407-3733
copyright: Authors who publish with this journal agree to the following terms: Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access). Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
items: Bovine Viral Diarrhea Virus Antigen and Immunoglobulin-G Detection in Unvaccinated Cattle
items: The Use of Selenium and Oestrous Synchronisation Protocols to Improve Oestrous Response and Fertility of Merino Ewes.
items: Potential Anticholesterol Infusion of Malaca Leaf (Phyllanthus Emblica) In Mice (Mus Musculus) Hypercholesterolemia
items: The Relationship between Knowledge and Attitude of Slaughterhouse Workers in Relation with Cattles' Fascioliasis as an Effort to Prevent Halzoun Syndrome in Jember
items: Kepadatan Lalat di Area Tempat Pengolahan Sampah Terpadu (TPST) Piyungan, Kabupaten Bantul, D.I. Yogyakarta
items: Pengaruh Hidrogen Peroksida (H2O2) dan Waktu Pengangkutan terhadap Respon Fisiologis Ikan Koi (Cyprinus carpio L.)
items: Detection of Newcastle Disease, Avian Influenza, dan Infectious Bronchitis Virus from Commercial with Respiratory Clinical Signs
items: Isolation of Aspergillus sp. from The Lungs of Sparrows (Passer montanus)
items: Isolasi dan Identifikasi Methicillin Resistant Staphylococcus aureus dan Methicillin Resistant Staphylococcus pseudintermedius pada Kasus Klinis Anjing dan Kucing di Yogyakarta
items: Antibacterial Activity of Turmeric (Curcuma domestica Val.) Extract on the Growth of Klebsiella sp.: In Vitro
items: Perilaku dan Gambaran Hematologis Beruk (Macaca nemestrina) yang Dipasang Bare metal stent pada Arteri Iliaca
items: Safety Risk Factors of Duck Meat Sold in Bogor City
items: Identification of Feather Mites on Asian Glossy Starling (Aplonis panayensis) in Industrial Estate
items: Potensi Kombinasi Rosemari (Rosemarinus officinalis), Eucalypytus (Eucalyptus pellita), dan Minyak Peppermin (Mentha piperita L.) pada Waktu Kematian Caplak
items: Aktivitas Ekstrak Etanol Daun Bandotan Terhadap Pertumbuhan Bakteri Vibrio sp. Isolat Ikan Lele Dumbo (Clarias gariepinus)
items: Economic Impact of Foot and Mouth Disease in Dairy Farmers at Koperasi Peternakan Bandung Selatan (KPBS) Pangalengan, Indonesia
items: Gejala Klinis dan Gambaran Darah pada Anjing Konjungtivitis
items: Kontaminasi Salmonella sp. pada Telur Ayam: Studi Komparatif Antara Peternakan dan Pasar Tradisional
items: Praktik Biosekuriti pada Pedagang Unggas Hidup di Pasar Tradisional di Bogor Terhadap Pencegahan Avian Influenza
items: Screening and Identification of Lactic Acid Bacteria from Livestock Rumen as Probiotic Candidates
items: Acute Anti-Inflammatory Study by Oral Administration of Cherry Leaf (Muntingia Calabura L.) Infusion in Inflammation-induced Wistar Strain White Rats (Rattus Norvegicus)
items: TINGKAT EFEKTIVITAS GLOVES STERIL INTRAOPERASI DAN PASCAOPERASI LAPAROTOMI
items: Dosis Efektif Anestesi Kombinasi Ketamine-xylazine pada Tikus Pediatri
items: Koleksi Semen pada Burung Parkit: Teknik Non-Invasif Pemijatan Kloaka
items: Penerapan Sanitasi dan Higiene dalam Produksi Ikan Asap: Implikasi terhadap Keamanan Pangan dan Kesehatan Masyarakat
items: SURVEI TATA LAKSANA TERAPI CAIRAN OLEH DOKTER HEWAN PRAKTISI HEWAN KECIL DI PROVINSI DKI JAKARTA DAN JAWA BARAT
items: Detection of Non-Structural Protein and Structural Protein Antibodies in Dairy Cattle Infected with Foot and Mouth Disease (FMD) in Boyolali, Central Java
items: ANGKA KEJADIAN DAN KARAKTERISTIK LESI PATOLOGI CALICIVIRUS PADA KUCING (Felis catus) DI VEE VET BANDUNG PERIODE AGUSTUS-SEPTEMBER 2024
items: Gambaran Makroskopis dan Histopatologi Hati Broiler yang Diinfeksi Escherichia coli dan Diberi Pakan Terapi Linkomisin-spektinomisin
items: Pengembangan Deteksi Parasit Darah Theileria equi dan Babesia caballi secara Otomatis menggunakan Algoritma YOLOv8

Bovine Viral Diarrhea Virus Antigen and Immunoglobulin-G Detection in Unvaccinated Cattle
type: item
description: To address the community's nutritional for meat can be supported by importing cattle while maintaining vigilance against Bovine Viral Diarrhea (BVD), an infectious disease caused by the Bovine Viral Diarrhea Virus (BVDV). This pathogen is associated with gastrointestinal, respiratory, and reproductive issues in cattle worldwide. Consequently, controlling BVDV stands as a paramount measure in preventing BVD. One effective strategy in this endeavor is vaccination. The successful implementation of vaccination relies on acquiring comprehensive information about BVDV antigens and antibodies present in the serum of unvaccinated cattle, which can provide insights into the protein profile resulting from natural infection. This study aimed to detect BVDV antigen and immunoglobulin-G (IgG) based on molecular weight in protein profiles from various cattle breeds imported into Indonesia. Utilizing the SDS-PAGE method, the analysis results revealed protein bands at 55.3 kDa and 151.3 kDa were detected in the serum of BVD-positive cows, meanwhile, these specific profiles were absent in BVD-negative cows. It can be concluded that the 55.3 kDa represents a BVDV antigen, while the 151.3 kDa corresponds to an IgG antibody profile. These findings can serve as one of the basic foundations for developing a BVD vaccine for cattle populations.
creator: Raden Rara Bhintarti Suryohastari
creator: Muharam Saepulloh
creator: Ana Roudlotul Jannah
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 203
ending page: 211
doi: 10.22146/jsv.101993

The Use of Selenium and Oestrous Synchronisation Protocols to Improve Oestrous Response and Fertility of Merino Ewes.
type: item
description: Selenium (Se) deficiency is linked to poor reproductive performance in sheep, while indiscriminate use of pharmaceuticals in oestrous synchronisation imposes economic burden on sheep farmers. Optimisation of interventions that could improve reproduction in sheep at minimum cost required investigation. This study evaluated the effect of Se-supplementation and oestrous synchronisation protocols on reproductive performance of Merino ewes and immunity of their lambs. Sixty ewes were randomly grouped in a 2x2 factorial design: Se-supplementation plus equine chorionic gonadotropin (eCG) (n=15), Se-supplementation plus male effect (n=15), non-Se-supplementation plus eCG (n=15) and non-Se-supplementation plus male effect (n=15). Ewes were hand mated with 8 Merino rams at 48 and 60 hours after CIDR removal. Data on oestrous response, distribution of oestrus, conception and lambing rates were analyzed using Chi-square test, while onset and duration of oestrus, gestation length, glutathione peroxidase, cortisol, oetradiol-17β, progesterone and Immunoglobulin G concentrations were analysed using PROC GLM with SAS®. Se-supplemented ewes had a higher (P<0.05) oestrous response at 36 hours after CIDR removal. Se-supplementation had no effect (P>0.05) on the onset, duration and distribution of oestrus, gestation length conception and lambing rate. Se-supplementation decreased (P<0.05) the cortisol concentration of ewes at CIDR removal and IgG concentration of lambs 12 hours after lambing. The eCG protocol increased (P<0.05) the number of ewes that displayed signs of oestrus at 24, 36 and 48 hours after CIDR removal compared to the male effect. Therefore, Se-supplementation combined with eCG administration improved oestrous response in Merino ewes and is more suitable for fixed-time artificial insemination.
creator: Sibonelo Mmelokuhle Hlatshwayo
creator: Ismaila Muritala
creator: Khoboso Christina Lehloenya
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 212
ending page: 225
doi: 10.22146/jsv.104279

Potential Anticholesterol Infusion of Malaca Leaf (Phyllanthus Emblica) In Mice (Mus Musculus) Hypercholesterolemia
type: item
description: The aim of this research was to find out infusion test of malaka leaves (Phyllantus emblica) to mice (Mus musculus) blood cholesterol rate which hypercholesterolemic mice. Twenty male mice with the age of 3 month and clinically healthy were used in this research. All mice were randomly divided into 4 treatment groups, 5 mice each. K1 was negative control group. K2 was positif control group, fed with hypercholesterolemic feed. K3 and K4 group were fed with hypercholesterolemic feed and infusion of malaka leaves with the doses of 0,04 and 0,08 mg body weight respectively. The result of statistic analysis showed that infusion of malaka leaves significantly effect (P<0,01) on the decrease of cholesterol level of male mice inducedby hypercholesterolemic feed. The treatment of infusion of malaka leaves 0,04 mg and 0,08 mg was able to decrease the cholesterol level ofmice induced by hypercholesterolemic feed. The advance test showed that the treatment K1 significantly effect (P<0,01) compare to K2 and K3. The K2 treatment significantly effect (P<0,01) with K1, K3, and K4. The K3 treatment showed no significanly effect compare to K4 (P<0,05). In conclusion, the administration of infusion of malaka leaves with the dose of 0,04 and 0,08 mg reduce cholesterol level.
creator: Sartika Silvana
creator: Nuzul Asmilia
creator: T. Armansyah TR
creator: Rasmaidar Rasmaidar
creator: M Isa
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 226
ending page: 232
doi: 10.22146/jsv.86890

The Relationship between Knowledge and Attitude of Slaughterhouse Workers in Relation with Cattles' Fascioliasis as an Effort to Prevent Halzoun Syndrome in Jember
type: item
description: Prevalensi fascioliasis pada sapi di Jember mencapai 56,6%, sehingga berpotensi menimbulkan kerugian ekonomi di Indonesia sekitar 6,9 miliar rupiah. Mengonsumsi hati sapi mentah atau setengah matang yang terkontaminasi cacing Fasciola spp. dewasa dapat menyebabkan sindrom halzoun, yaitu reaksi hipersensitivitas yang mengenai mukosa bukafaring dan saluran pernapasan atas. Pencegahan sindrom halzoun yang efektif memerlukan penerapan prinsip “ASUH” oleh pekerja rumah potong hewan, yang harus memiliki pengetahuan yang baik dan sikap proaktif terhadap tugasnya. Penelitian ini bertujuan untuk mengkaji hubungan antara pengetahuan dan sikap pekerja rumah potong hewan dengan prevalensi fascioliasis pada sapi sebagai upaya pencegahan sindrom halzoun di Jember. Desain penelitian yang digunakan adalah cross-sectional, dengan pengumpulan data dilakukan pada 35 pekerja di 9 rumah potong hewan di Jember pada bulan Agustus sampai dengan Desember 2024. Analisis menggunakan Uji Fisher's Exact. Hasil analisis menunjukkan nilai p untuk pengetahuan dan sikap terhadap fascioliasis pada sapi masing-masing adalah p=0,103 dan p=0,592. Kesimpulan dari penelitian ini adalah tidak terdapat hubungan yang signifikan antara pengetahuan dan sikap petugas rumah potong hewan dengan prevalensi fascioliasis pada sapi sebagai upaya pencegahan sindrom halzoun di Jember.
creator: Bagus Hermansyah
creator: Safira Putri Nur Salsabilla
creator: Zahrah Febrianti
creator: Wiwien Sugih Utami
creator: Yunita Armiyanti
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 233
ending page: 241
doi: 10.22146/jsv.102910

Kepadatan Lalat di Area Tempat Pengolahan Sampah Terpadu (TPST) Piyungan, Kabupaten Bantul, D.I. Yogyakarta
type: item
description: Lalat merupakan Arthropoda yang termasuk Ordo Diptera dengan karakteristik tubuh bersegmen-segmen. Lalat merupakan vektor food-borne disease yang dapat menyebabkan penyakit diare, disentri, typhus, dan juga myiasis. Umumnya keberadaan sampah sesuai untuk lalat hidup dan melanjutkan perkembangbiakannya. Lalat dapat bertahan hidup di Tempat Pembuangan Sampah Terpadu (TPST). Penelitian ini bertujuan untuk mengetahui kepadatan dan spesies lalat di TPST Piyungan dan permukiman sekitarnya. Penelitian dilakukan pada Juli–September 2021. Lalat dikoleksi dengan menggunakan sweep net dan dihitung kepadatannya dengan fly grill. Lalat yang sudah dikoleksi kemudian diidentifikasi dengan buku identifikasi “Borror and Delong’s Introduction To The Study of Insect” (Triplehorn and Johnson, 2005). Analisis keanekaragaman dilakukan dengan indeks keanekaragaman Shannon-Wiener dan pola distribusi menggunakan Indeks Morisita. Hasil penelitian menunjukkan bahwa kepadatan lalat di TPST Piyungan dan permukiman sekitarnya termasuk tinggi yaitu masing-masing sebesar 68 dan 43,6. Indeks keanekaragaman di TPST Piyungan temasuk rendah dengan nilai sebesar 0,37 dan pada permukiman sebesar 1,07 yang berarti sedang. Ditemukan spesies lalat masing- masing berjumlah 4 dan 5 di TPST Piyungan dan area permukiman sekitarnya. Spesies lalat yang ditemukan berupa Musca domestica, Fannia sp., Chrysomya megacephala, Lucilia sp., dan Sarcophaga sp. Pola distribusi kelima spesies lalat sendiri tergolong kedalam penyebaran secara mengelompok. Pola distribusi lalat dipengaruhi oleh faktor seperti suhu sebesar 28-29,5⁰C dan kelembaban berkisar 69%-78% yang optimal untuk hidup lalat.
creator: Raden Roro Upiek Ngesti Wibawaning Astuti
creator: Dila Hening Windyaraini
creator: Ratriana Kartikasari
creator: Nayla Humaira Maheswari
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 242
ending page: 254
doi: 10.22146/jsv.74449

Pengaruh Hidrogen Peroksida (H2O2) dan Waktu Pengangkutan terhadap Respon Fisiologis Ikan Koi (Cyprinus carpio L.)
type: item
description: Ikan koi (Cyprinus carpio L.) merupakan salah satu komoditas ikan hias air tawar introduksi, yang sampai saat ini masih menjadi primadona di pasar internasional dan termasuk jenis ikan hias yang bernilai jual tinggi dengan harga yang relatif stabil. Penelitian ini bertujuan untuk menyelidiki pengaruh pemberian hidrogen peroksida dan waktu pengangkutan terhadap respon fisiologis ikan koi serta interaksi antara hidrogen peroksida dan waktu pengangkutan. Metode yang digunakan Rancangan Acak Lengkap Faktorial dua faktor dan tiga kali ulangan. Ikan koi (panjang 10,70+0,97 cm dan berat 14,45+0,21 gram, n = 540 ekor) digunakan dalam penelitian ini. Faktor pertama (A) yaitu konsentrasi H2O2 sebanyak enam taraf (A1) 0,15 ml/l, (A2) 0,35 ml/l, (A3) 0,55 ml/l, (A4) 0,75 ml/l, (A5) 0,95 ml/l, dan (A6) kontrol. Sedangkan faktor kedua (B) waktu pengangkutan sebanyak tiga taraf yaitu (B1) 10 jam, (B2) 15 jam, (B3) 20 jam. Parameter yang diukur adalah sintasan, kadar kortisol, glukosa, histologi hati, penilaian pasca pengangkutan, dan kualitas air. Hasil penelitian menunjukan bahwa interaksi konsentrasi hidrogen peroksida dan waktu pengangkutan berpengaruh nyata terhadap sintasan, kortisol, dan glukosa. Berdasarkan hasil pengujian, perlakuan terbaik diperoleh pada perlakuan A1B1 dengan konsentrasi hidrogen peroksida sebesar 0,15 ml/l dan waktu pengangkutan 10 jam, yang menghasilkan sintasan sebesar 100 %, kadar kortisol sebesar 0,45 ng/ml, dan kadar glukosa sebesar 56,80 mg/dL, tidak terdapat kerusakan pada jaringan.
creator: Dien Uma Nurina
creator: Sinar Pagi Sektiana
creator: I Ketut Sugama
creator: Putu Angga Wiradana
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 255
ending page: 266
doi: 10.22146/jsv.98831

Detection of Newcastle Disease, Avian Influenza, dan Infectious Bronchitis Virus from Commercial with Respiratory Clinical Signs
type: item
description: Clinical symptoms in the chicken’s respiratory system, such as sores, nasal discharge, gasping, and so on, can be caused by a variety of possible socks. These signs may be caused by a virus, usually followed by economic losses. Causative diagnosis, especially virus detection, is needed to establish the cause of the symptoms. The study aims to detect Newcastle Disease (ND), Avian Influenza (AI), and Infectious Bronchitis (IB) cases of respiratory symptoms in commercial chickens by 2023. The samples studied in this study were organs from commercial chicken broilers and layers with respiratory symptoms. The organ was prepared as a suspension, then centrifuged to take out the supernatants. Supernatants were extracted using commercial extraction kits to produce templates for molecular detection. Molecular detection was performed using the RT-PCR two-step method. Primer pairs were used for the detection of ND, AI subtypes H5 and H9, as well as IB. The results were visualized by electrophoresis with 1.5% agarose. Interpretation of the positive result of RT-PCR based on the appearance and length of amplicons compared with the positive control of each virus. Positive RT-PCR samples with thick amplicon quality were subjected to sequencing and bioinformatics analysis. Results of detection with RT-PCR against 41 samples are positive ND of 1 out of 8 samples (12.5%), AI of 2 out of 17 samples (11.7%), and IB of 5 out of 16 samples (31.3%). Based on the molecular detection with RT-PCR, the symptoms of respiration in commercial chickens are confirmed due to the cause, namely the virus: ND genotype VII-i, AI subtype H5 clade 2.3.2, AI subtype H9, and IB genotype GI-19 (QX-like).
creator: Ferdinand Prayogo Cahyo Santoso
creator: Agnesia Endang Tri Hastuti Wahyuni
creator: Tri Untari
creator: Widya Asmara
creator: Michael Haryadi Wibowo
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 267
ending page: 276
doi: 10.22146/jsv.93221

Isolation of Aspergillus sp. from The Lungs of Sparrows (Passer montanus)
type: item
description: Aspergillosis is a common respiratory disease in avian species. The infection of Aspergillus sp. is characterized by the presence of yellowish plaques or nodules in the respiratory tract such as the trachea, air sac, and lungs. This study aimed to isolate Aspergillus sp. from the lungs of sparrows. The samples used in this research were the lungs of 35 sparrows taken randomly. Isolation was carried out by Thompson’s method (1969), samples were embedded in Saboraud’s Dextrose Agar (SDA) media and then incubated for 2-7 days. The growth of Aspergillus sp. colonies was observed at 24-hour intervals by looking at the color, shape, edges, and bottom surface of the colonies. Meanwhile, to ensure and confirm the species of Aspergillus sp., the fungus was planted on slide culture media. After that, microscopic observations were conducted by looking at the growth of conidiophores, hyphae, phialids, and fungal conidia. The data obtained were then analyzed descriptively. The result showed that Aspergillus sp. can be isolated from 27 out 35 samples. Based on the result of this study it can be concluded that 77.14% of samples were infected by Aspergillus sp. consisting of Aspergillus niger and Aspergillus flavus.
creator: Ronaldi Fajar Nugraha
creator: Erina Erina
creator: M. Daud AK
creator: Faisal Jamin
creator: Darniati Darniati
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 277
ending page: 282
doi: 10.22146/jsv.102705

Isolasi dan Identifikasi Methicillin Resistant Staphylococcus aureus dan Methicillin Resistant Staphylococcus pseudintermedius pada Kasus Klinis Anjing dan Kucing di Yogyakarta
type: item
description: Methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP), are known as bacterial resistant to various antibiotics. MRSA transmission occurs between humans and direct human contact with dogs and cats. This study aimed to isolate, characterize, and analyze bacterial resistance to antibiotics phenotypically and genotypically from clinical case samples of pet animals in Yogyakarta. Bacterial isolates were identified through biochemical tests and molecular identification by species-specific 23S rRNA and nuc genes with polymerase chain reaction (PCR) and pta genes with PCR-restriction fragment length polymorphism (RFLP). Bacterial resistance to several antibiotics was analyzed using disk diffusion Kierby-Bauer method. The occurrence of methicillin resistance were screened by oxacillin resistance screening agar base (ORSAB) and confirmed by detection of the mecA gene with PCR. Based on the phenotypic and genotypic identification showed that 11 samples were identified as S. aureus (6 isolates) and S. pseudintermedius (5 isolates). The resistance tests revealed that 36% were resistant to tetracycline, cefoxitin (27%), oxacillin and erythromycin (9%). Screening of methicillin resistance with ORSAB indicated that all isolates were methicillin resistances (100%). Detecting the mecA gene encoding methicillin resistance showed that 81.81% of isolates were resistant to methicillin, with detail S. aureus (all 6 isolates) and S. pseudintermedius (4 isolates). Based on the antibiogram, ORSAB, and mecA gene detection, it could be confirmed that three isolates were MRSP, and six isolates were MRSA. The results of this study indicate the high occurrence of methicillin-resistant strains in pets that have the potential zoonotic spread to other animals and humans.
creator: Ghias Ghifari Alhadz
creator: Siti Isrina Oktavia Salasia
creator: Alyaa Rifqoh Putri Yosyana
creator: Madarina Wasissa
creator: Fajar Budi Lestari
creator: Rini Widayanti
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 283
ending page: 291
doi: 10.22146/jsv.100240

Antibacterial Activity of Turmeric (Curcuma domestica Val.) Extract on the Growth of Klebsiella sp.: In Vitro
type: item
description: Klebsiella sp. is one of the causes of bacterial diseases that attack large farm animals and poultry. Treatment of infections caused by these bacteria is using antibiotics. Cases of antibiotic resistance against Klebsiella sp. encouraging researchers to study spice plants as antibacterial, especially turmeric (Curcuma domestica Val.). Research related to the antibacterial activity of turmeric against Klebsiella spp. not much has been done. Therefore, this study aimed to determine the antibacterial activity of turmeric extract against Klebsiella sp. in vitro. re-identified by testing on MacConkey agar, triple sugar iron agar, and IMViC (indole, methyl red, Voges-Proskauer, citrate). Overall results showed the characteristics of Klebsiella sp. Making turmeric powder simplicia extract by maceration method followed by evaporation using a spray dryer. Turmeric extract was diluted with dimethyl sulfoxide (DMSO). The antibacterial activity test of turmeric extract (20%, 40%, 60%, 80%, 100%) was carried out on Mueller-Hinton agar that had been given wells. Chloramphenicol (30 g) was used as a positive control and DMSO as a negative control. The test was carried out with three repetitions. The mean inhibition zone diameter of three repetitions was 20.00 mm; 0.00mm; 10.17mm; 10.67 mm; 10.67 mm; 11.17 mm and 12.17 mm for positive control, negative control, 20-100% turmeric extract. Based on the results of phytochemical tests, turmeric extract contains flavonoids, alkaloids, quinones and triterpenoids. Turmeric extract with concentrations of 20%, 40%, 60%, 80% and 100% had the activity to inhibit the growth of Klebsiella sp. with a strong category on in vitro testing.
creator: Aprilia Hardiati
creator: Muchamad Armand Satriani
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-01
publication date: 2025-08-01
volume: 43
number: 2
starting page: 292
ending page: 297
doi: 10.22146/jsv.73639

Perilaku dan Gambaran Hematologis Beruk (Macaca nemestrina) yang Dipasang Bare metal stent pada Arteri Iliaca
type: item
description: Penyakit kardiovaskuler yang paling banyak diderita oleh masyarakat Indonesia adalah penyakit jantung coroner. Salah satu metode terapi jantung korner adalah dengan pemasangan stent jantung. Saat ini stent jantung yang digunakan oleh kardiolog di Indonesia masih mengandalkan produk impor. Saat ini Universitas Gadjah Mada (UGM) sedang mengembangkan stent dengan desain dan bahan yang telah diteliti sebelumnya yang disebut dengan INA-Stent. Tahap pre-klinis telah dilaksanakan dengan menggunakan hewan beruk (Macaca nemestrina). Pemasangan bare metal stent (tanpa salut obat) telah dipasang pada bulan September tahun 2018. Proses euthanasia dilaksanakan pada bulan September 2021 di ruang kateterisasi Rumah Sakit Hewan pendidikan Prof. Soeparwi Fakultas Kedokteran Hewan (FKH UGM). Sebelum pelaksanaan euthanasia diambil sampel darah sebanyak 1 ml pada tabung ethylene diamine tetra acetic acid (EDTA). Pemeriksaan darah menunjukkan total eritrosit, hemoglobin, PCV, MCV, total leukosit, dan trombosit dalam batas normal. Abnormalitas yang terjadi antara lain limfopenia, monositosis dan hipokromia.
creator: Dinar Arifianto
creator: Agustina Dwi Wijayanti
creator: Setyo Budhi
creator: Heri Wijayanto
creator: Widowati Siswomihardjo
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-01
publication date: 2025-08-01
volume: 43
number: 2
starting page: 298
ending page: 304
doi: 10.22146/jsv.70940

Safety Risk Factors of Duck Meat Sold in Bogor City
type: item
description: With the increasing demand for duck meat in Bogor City, this poses a risk of the presence of pathogenic bacteria throughout the food supply chain. Traditional markets are one of the places that have a high risk of contamination with pathogenic bacteria that can endanger public health. This study aims to determine the presence of Salmonella and E. coli in duck meat sold in traditional markets in Bogor City and the risk factors that influence the presence of these pathogenic bacteria. This study was a cross sectional study with a total of 45 duck meat samples obtained from 3 traditional markets in Bogor City. Samples were taken to the SKHB IPB Veterinary Public Health Laboratory for microbiological testing. Risk factors that may affect the safety of duck meat were assessed using a questionnaire. The results showed that 13 samples (29%) were positive for Salmonella and 27 samples (60%) were positive for E. coli. The presence of Salmonella and E. coli in duck meat indicates cross-contamination during slaughter, evisceration and poor sanitation and hygiene practices. Continuous monitoring and reporting of the presence of Salmonella and E. coli is needed so that duck meat consumed by the public is safe for consumption.
creator: rizka fajri annisa
creator: Trioso Purnawarman
creator: Herwin Pisestyani
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-01
publication date: 2025-08-01
volume: 43
number: 2
starting page: 305
ending page: 315
doi: 10.22146/jsv.101423

Identification of Feather Mites on Asian Glossy Starling (Aplonis panayensis) in Industrial Estate
type: item
description: This study aims to identify the types of feather mites found on Asian glossy starling (Aplonis panayensis) (Passeriformes: Sturnidae) as a pest in industrial estate. This issue has not been extensively studied, and information about the feather mites biodiversity of Asian glossy starlings in Indonesia is limited. Feather mites samples were collected from KIE Bontang, East Kalimantan in January 2024. Live traps with mist nests were used to catch Asian glossy starlings and mites were examined on the feathers. Mites specimens preserved in ethanol 70%. Asian glossy starlings then released into the wild after examination. Data analysis and mites identification were conducted in the Health Entomology Laboratory of the School of Veterinary Medicine and Biomedicine at Bogor Agricultural University from July until September 2024. Feather mites were preserved on the microscope slide. Mites observations were used Olympus CX23 microscope with 10x and 40x objective magnifications. Based on identification, feather mites on Asian glossy starlings were described 2 types Astigmata, Trouessartia sp. (Sarcoptiformes: Trouessartiidae), and Montesauria sp. (Sarcoptiformes: Proctophyllodidae) found on the secondary feathers of Asian glossy starling wings. Male Trouessartia sp. was found with patterned shields, like hollow tissue (lacunae) and anterior hysterosomal shield separate from the other shield. Female Montesauria sp. was found with a slender, elongated, and flat body shape, large and sclerotized dorsal body shield, leg I hypertrophied, setae h2 fusiform and spindle-like, filiform setae h3. Trouessartia sp. and Montesauriasp. play an important role in maintaining ecosystem function as symbionts.
creator: Jasti Rahayu
creator: Susi Soviana
creator: Upik Kesumawati Hadi
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-01
publication date: 2025-08-01
volume: 43
number: 2
starting page: 316
ending page: 321
doi: 10.22146/jsv.102254

Potensi Kombinasi Rosemari (Rosemarinus officinalis), Eucalypytus (Eucalyptus pellita), dan Minyak Peppermin (Mentha piperita L.) pada Waktu Kematian Caplak
type: item
description: Caplak menjadi faktor penyebab gangguan kulit yang banyak terjadi pada anjing. Resiko akibat caplak membuat kulit anjing mengalami kerusakan jaringan dan reaksi inflamasi sehingga penampilan terlihat jelek. Berbagai metode penaggulangan infeksi caplak telah berkembang. Namun, perlu adanya penyediaan obat baru yang lebih efektif. Penelitian ini bertujuan menguji waktu kematian caplak terhadap kombinasi rosemari, eucalyptus, dan minyak peppermin. Caplak Rhipicephalus sanguineus diperoleh melalui teleskopik dari anjing yang positif terinfeksi caplak di Asrama Mahasiswa Papua Dramaga Bogor. Perlakukan yang digunakan dengan komposisi rosemari 2 ml, eucalypytus 2 ml, dan minyak peppermin 1 ml; rosemari 2 ml, eucalypytus 2 ml, dan minyak peppermin 1,5 ml; serta rosemari 2 ml, eucalypytus 2 ml, dan minyak peppermin 2 ml dengan berlipat ganda sebanyak tiga kali. Analisis data dilakukan dengan melihat jumlah mortalitas akibat pengaruh paparan konsentrasi yang berbeda pada kombinasi rosemari, eucalyptus, dan minyak peppermin terhadap mortalitas caplak. Komposisi rosemari 2ml, eucalypytus 2 ml, dan minyak peppermin 2 ml pada hasil penelitian merupakan kombinasi paling efektif membunuh caplak dibandingkan 2 kombinasi lainnya.
creator: Bayu Febram Prasetyo
creator: Rini Madyastuti Purwono
creator: Adrian Saragih
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-01
publication date: 2025-08-01
volume: 43
number: 2
starting page: 322
ending page: 325
doi: 10.22146/jsv.91468

Aktivitas Ekstrak Etanol Daun Bandotan Terhadap Pertumbuhan Bakteri Vibrio sp. Isolat Ikan Lele Dumbo (Clarias gariepinus)
type: item
description: Daun bandotan merupakan salah satu tanaman obat yang banyak dimanfaatkan dalam mengobati berbagai jenis penyakit karena mengandung senyawa fitokimia yang dapat menghambat pertumbuhan bakteri seperti alkaloid, flavonoid, tanin, saponin dan steroid. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol daun bandotan terhadap pertumbuhan bakteri Vibrio sp. isolat ikan lele dumbo. Pelaksanaan penelitian ini menggunakan metode Kirby Bauer disk diffusion yang diawali dengan peremajaan stok isolat bakteri pada media Nutrient Broth, reidentifikasi dengan media Thiosulfate Citrate Bile Salt Sucrose Agar dan pewarnaan Gram. Selanjutnya dilakukan uji daya hambat menggunakan media Mueller Hinton Agar. Studi ini terdiri dari 5 perlakuan yaitu ekstrak etanol daun bandotan konsentrasi 25% (P1), 50% (P2), dan 75% (P3), siprofloksasin sebagai kontrol positif dan CMC 1% sebagai kontrol negatif. Hasil penelitian menunjukkan diameter zona hambat yang terbentuk pada kontrol positif (45,13 ± 0,04mm) dua kali lebih besar dibandingkan daya hambat yang terbentuk pada ekstrak daun bandotan konsentrasi 25% (23,30 ± 0,15mm), konsentrasi 50% (24,29 ± 0,17 mm), dan konsentrasi 75% (25,29 ± 0,16mm), kontrol negatif tidak menunjukkan terbentuknya zona hambat. Kesimpulan penelitian adalah ekstrak etanol daun bandotan berpotensi dapat menghambat pertumbuhan bakteri Vibrio sp. hasil isolat lele dumbo.
creator: Masda Admi
creator: Khairani Fitri
creator: Hennivanda Hennivanda
creator: Faisal Jamin
creator: Rosmaidar Rosmaidar
creator: Syafruddin Syafruddin
creator: Winaruddin Winaruddin
creator: Azhari Azhari
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-01
publication date: 2025-08-01
volume: 43
number: 2
starting page: 326
ending page: 333
doi: 10.22146/jsv.96568

Economic Impact of Foot and Mouth Disease in Dairy Farmers at Koperasi Peternakan Bandung Selatan (KPBS) Pangalengan, Indonesia
type: item
description: This study aims to analyze the economic impact of the foot and mouth disease (FMD) outbreak on dairy farmers who are members of the Pangalengan South Bandung Livestock Cooperative (KPBS). Data were collected through structured questionnaires, in-depth interviews, and field observations of 100 FMD-affected farmers during September-November 2023, with follow-up evaluation one year after the outbreak. The results showed that during the outbreak (29 days), the average loss per farmer reached IDR 44,018,068 or IDR 6,288,295 per head, mostly due to decreased milk production, dairy cow mortality, and disease control costs, including treatment, vaccination, and disinfection. One year after the outbreak (365 days), economic losses were IDR 46,077,090 or IDR 6,582,441 per head, dominated by the residual impact of FMD in the form of a 39.75% reduction in milk yield (18.31 kg/day). Economic losses also involved additional costs, such as vector control and livestock burial, which further burdened farmers. These findings highlight the importance of more effective FMD control policies, including mass vaccination, improved biosecurity and financial assistance for affected farmers, to reduce economic impacts and support long-term recovery.
creator: Mas Taufiqqurrahman
creator: Etih Sudarnika
creator: Denny Widaya Lukman
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 334
ending page: 344
doi: 10.22146/jsv.103474

Gejala Klinis dan Gambaran Darah pada Anjing Konjungtivitis
type: item
description: Konjungtivitis merupakan peradangan pada konjungtiva mata yang dapat disebabkan oleh agen infeksi, alergi atau faktor lingkungan. Gejala klinis konjungtivitis bervariasi dan dapat disertai perubahan gambaran darah. Tujuan penelitian ini adalah untuk mengetahui gejala klinis dan gambaran darah pasien anjing penderita konjungtivitis. Penelitian ini menggunakan 8 sampel anjing yang mengalami konjungtivitis. Semua anjing diperiksa secara klinis, terutama bagian mata, serta diambil sampel darah untuk diperiksa gambaran darahnya. Hasil pemeriksaan klinis anjing konjungitivitis, ditemukannya 8 (100%) pasien mengalami hiperemia, inflamasi, dan hiperlakrimasi, 6 (75%) pasien terdapat eksudat, dan 1 (12,5%) pasien mengalami kerusakan mata. Hasil pemeriksaan darah rutin menunjukkan 1 (12,5%) sampel mengalami penurunan nilai MCHC. Jumlah leukosit, limfosit, monosit, granulosit, dan trombosit dari 8 sampel berada pada kisaran normal. Disimpulkan bahwa anjing penderita konjungtivitis menunjukkan gejala klinis terutama adalah hiperemi, inflamasi, adanya eksudat, dan hipelakrimasi pada mata dengan gambaran darah yang normal.
creator: Habib Prasetyo Harjono
creator: Soedarmanto Indarjulianto
creator: Agung Budi Pramono
creator: Ida Tjahajati
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 345
ending page: 349
doi: 10.22146/jsv.103749

Kontaminasi Salmonella sp. pada Telur Ayam: Studi Komparatif Antara Peternakan dan Pasar Tradisional
type: item
description: Eggs are a food ingredient consumed daily by the community; eggs infected with Salmonella sp. can cause various health problems. The global prevalence rate of salmonellosis disease based on data from the Institute for Health Metrics and Evaluation in 2019 stated that it was 4.22 per 100,000 population with a mortality rate due to salmonellosis of 1.72%. The end of this study was to see the comparison of Salmonella sp. contamination in chicken eggs in terms of differences in location on farms and in the Sukowono market. This study included 70 randomly selected chicken eggs from two different locations, namely chicken farms and traditional markets in Sukowono sub-district, using the egg shell swab method, and then bacterial culture was carried out using SSA (Salmonella Shigella Agar). The prevalence of Salmonella sp. contamination in the chicken eggs examined was 25.7%. There was a significant difference in the comparison of Salmonella sp. contamination in chicken eggs in terms of differences in location on farms and market traders. Eggs from farms have lower Salmonella sp. contamination compared to eggs from traditional markets.
creator: Nanda Dewi Anggraini
creator: Enny Suswati
creator: Yohanes Sudarmanto
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 350
ending page: 355
doi: 10.22146/jsv.105836

Praktik Biosekuriti pada Pedagang Unggas Hidup di Pasar Tradisional di Bogor Terhadap Pencegahan Avian Influenza
type: item
description: Penyakit avian influenza (AI) merupakan penyakit zoonotik yang mengancam kesehatan manusia. Unggas hidup yang dijual di di pasar tradisional merupakan berpotensi menjadi sumber penularan virus AI, sehingga dibutuhkan langkah pencegahan dan pengendalian penyakit di lokasi tersebut. Penelitian ini bertujuan untuk mengkaji praktik biosekuriti pada pedagang unggas hidup di wilayah Bogor sebagai langkah pencegahan penularan penyakit AI. Penelitian dilakukan melalui wawancara menggunakan kuesioner yang disusun sebagai alat untu mengidentifikasi karakteristik pedagang unggas dan penerapan praktik biosekuriti. Sebanyak 30 pedagang diwawancarai sebagai responden di 3 pasar tradisional di Kota Bogor dan 7 pasar tradisional di Kabupaten Bogor. Data dianalisis secara deskriptif untuk mengetahui gambaran terkait karakteristik pedagang unggas serta praktik biosekuriti yang dilakukan pedagang. Hasil penelitian menunjukkan praktik biosekuriti terhadap penyakit AI belum sepenuhnya diterapkan oleh pedagang unggas di wilayah Bogor, terutama pada aspek isolasi hewan sakit dan sanitasi. Selain itu kesadaran pedagang unggas untuk melaporkan kejadian penyakit masih sangat rendah (20%). Penerapan praktik biosekuriti pada pedagang perlu ditingkatkan melalui penyuluhan terkait penyakit AI serta perbaikan sarana dan prasarana kios unggas. Kata kunci : Avian Influenza, Biosekuriti, Pedagang unggas hidup, Kios unggas
creator: Falih Prenata Saukhan
creator: Etih Etih Sudarnika Sudarnika
creator: Denny Widaya Lukman
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 356
ending page: 368
doi: 10.22146/jsv.104820

Screening and Identification of Lactic Acid Bacteria from Livestock Rumen as Probiotic Candidates
type: item
description: Population of lactic acid bacteria (LAB) in the rumen of fattening livestock is relatively high and has the potential to be utilized. This study aimed to screen and identify LAB from the rumen as probiotic candidates. The research used rumen liquid from cattle, goats, and sheep collected from slaughterhouses in Bondowoso. The screening and identification processes were conducted at the Livestock Agroindustry Laboratory, Faculty of Agriculture, University of Jember. The screening included colony morphology, Gram staining, cell shape, catalase test, and Carbon dioxide (CO2) gas production ability. The final identification of potential probiotic bacterial candidates was based on their bile salt tolerance. The results showed that there were LAB candidates with 22 isolates (from sheep/OS), 19 isolates (from cattle/OC), and 18 isolates (from goats/OG). The bacterial colony shape included oval (2OS, 1OC), circular (11OS, 18OC, 12OG), irregular (7OS, 6OG), and spindle (2OS). Colony sizes ranged from small (0.5–1.2 mm: 7 OS, 9OC, 4OG), medium (1.2–3.0 mm: 7OS, 7OC, 7OG), to large (3.0–5.0 mm: 8OS, 3OC, 7OG), with the majority of colonies being white and cream-colored, and a minority being yellow. All bacteria were Gram-positive, with coccus (19OS, 14OC, 2OG) and bacillus (3OS, 5OC, 16OG) shaped cells. All samples were capable of producing CO2 gas, and 50% of the isolates possessed catalase enzymes. Based on bile salt tolerance tests, 15% of the isolates had a survival rate of over 50%. In conclusion, LAB from the rumen, particularly goats, were identified as potential probiotic candidates for further development.
creator: Desy Cahya Widianingrum
creator: Ahmad Ilham Tanzil
creator: Rachmi Masnilah
creator: Suhartiningsih Dwi Nurcahyanti3
creator: Vivi Fitriani
creator: Himmatul Khasanah
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 369
ending page: 377
doi: 10.22146/jsv.104307

Acute Anti-Inflammatory Study by Oral Administration of Cherry Leaf (Muntingia Calabura L.) Infusion in Inflammation-induced Wistar Strain White Rats (Rattus Norvegicus)
type: item
description: Inflammation is a response of the body’s defense system in preparation for tissue repair. Acute inflammation is characterized by heat, redness, pain, and formation of edema. Acute inflammation involve the realese of inflammatory mediators. Muntingia calabura is a herbal plant commonly used as an anti-inflammatory medicine containing bioactive coumpounds such as flavonoids, triterpenoids, and alkaloids that can inhibit the regulation of inflammatory mediators. This study aims to determine the anti-inflammatory activity and optimal dosage of M. calabura infusion in addressing acute inflammation in rats. The research method is a laboratory experiment using Wistar strain white rats that divided into 7 treatment groups: normal, negative control, positive control (sodium diclofenac and methylprednisolone), and M. calabura infusion at doses of 150, 300, and 600 mg/kg body weight. Each rats model induced with 1% carrageenan at 0.2 ml intraplantar, the formed edema is measured using a plethysmometer every 30 minutes for the first 3 hour and every 60 minutes for the following 3 hour. The result indicate that M. calabura infusion has acute anti-inflammatory effect on white rats model induced with carrageenan, with the most optimal dose being 600 mg/kg body weight, showing a pattern of anti-inflammatory activity similar to methylprednisolone.
creator: Putra Ramadhan
creator: Enny Rohmawaty
creator: Aziiz Mardanarian Rosdianto
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 378
ending page: 385
doi: 10.22146/jsv.92910

TINGKAT EFEKTIVITAS GLOVES STERIL INTRAOPERASI DAN PASCAOPERASI LAPAROTOMI
type: item
description: Penggunaan glove bedah steril untuk tindakan laparotomi dilakukan untuk membatasi kontaminasi lokasi pembedahan oleh bakteri di tangan operator bedah dan dengan demikian mengurangi kemungkinan infeksi pascaoperasi. Pada prakteknya pergantian gloves steril selama proses operasi di kalangan praktisi dokter hewan jarang bahkan tidak pernah dilakukan. Tujuan penelitian ini adalah mengetahui tingkat efektifitas penggunaan gloves steril selama proses operasi (intraoperasi) dan post operasi ovariohisterektomi dengan melihat total kuantitas bakteri yang didapatkan pada sampel gloves steril. Dua belas sampel dibagi menjadi tiga kelompok perlakuan. Masing-masing kelompok terdiri dari 4 sampel. Kelompok 1 adalah kontrol, swab pada gloves steril dilakukan sebelum operasi dimulai. Kelompok 2 adalah swab pada gloves steril yang dilakukan pada menit ke 30 setelah operasi dimulai. Variabel 3 adalah swab pada gloves steril yang diambil pada menit ke 60 (post operasi). Durasi operasi ovariohisterektomi yang dilakukan berlangsung selama 48 menit. Swab dilakukan dengan menggunakan sterile swabs yang sudah dibasahi menggunakan NaCl steril, kemudian permukaan gloves dengan luas sekitar 5 cm x 5 cm diusab dan sampel direndam dalam konikel berisi NaCl steril. Sampel kemudian dikirim ke Departemen Kesehatan Masyarakat Veteriner FKH UGM untuk pengujian Total Plate Count (TPC). Data yang diperoleh selanjutnya diuji statistik menggunakan one way ANOVA. Hasil pemeriksaan TPC menggunkan analisis statistik one way ANOVA. Hasil penelitian menunjukkan bahwa CFU/ml glove control (4550±5193,9), glove pada menit ke-30 operasi (10075±14747,5) dan glove setelah 60 menit operasi (27000±48764,7). Kesimpulan, tingkat efektifitas glove steril pada menit ke-0, ke-30 dan ke-60 operasi tidak menunjukkan perubahan yang signifikan (P>0.05) terhadap jumlah bakteri. Dengan demikian, tidak adanya perubahan signifikan jumlah koloni bakteri selama 48 menit selama operasi berlangsung dan post operasi (menit ke-60), ma…
creator: Artina Prastiwi
creator: Dhirgo Adji
creator: Devita Anggraeni
creator: Agus Purnomo
creator: Mungky Ema Ramadhani
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 386
ending page: 393
doi: 10.22146/jsv.105484

Dosis Efektif Anestesi Kombinasi Ketamine-xylazine pada Tikus Pediatri
type: item
description: Anestesi efektif mengurangi stres dan rasa sakit pada tikus dengan menghilangkan kesadaran. Kombinasi yang umum digunakan dalam penelitian adalah Ketamine dan Xylazine. Kombinasi ini telah banyak digunakan pada tikus dewasa dengan berbagai dosis, tetapi belum ada acuan dosis tepat untuk tikus pediatri. Penelitian ini bertujuan mendapatkan dosis anestesi dari kombinasi Ketamine-Xylazine yang efektif dan aman untuk tikus pediatri. Penelitian menggunakan 25 ekor tikus berumur 3 minggu yang dibagi menjadi 5 kelompok perlakuan, yaitu kelompok negatif dan 4 perlakuan dosis Ketamine (25, 50, 75, dan 100 mg/kg BB) dikombinasikan dengan Xylazine 4 mg/kg BB. Hasil penelitian menunjukkan efek anestesi tikus pediatri baru mulai muncul setelah pemberian dosis Ketamine 50 mg/Kg BB. Kondisi fisiologis (frekuensi jantung, frekuensi napas, dan suhu tubuh) mengalami penurunan pada 10 menit setelah injeksi anestesi dan seiring waktu berangsur kembali ke normal. Pada dosis yang tinggi (100 mg/kg BB), terjadi kematian pasca injeksi dengan tingkat kematian 40%. Dosis anestesi Ketamine-Xylazine yang disarankan adalah 50–75/4 mg/kg BB.
creator: Muhammad Luthfi Rahman
creator: Titiek Sunartatie
creator: Altaff Hendry
creator: Teo Qin Yan
creator: Dian Maulia Utami
creator: Daniel Latief Andre
creator: Win Satya Rudramurti
creator: Diah Nugrahani Pristihadi
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-01
publication date: 2025-08-01
volume: 43
number: 2
starting page: 394
ending page: 401
doi: 10.22146/jsv.99829

Koleksi Semen pada Burung Parkit: Teknik Non-Invasif Pemijatan Kloaka
type: item
description: Koleksi semen merupakan ejakulasi yang dipengaruhi oleh faktor eksternal seperti lingkungan, perlakuan, dan alat koleksi (Madeddu et al., 2022; Vitorino Carvalho, 2024). Koleksi semen pada burung parkit dapat dilakukan dengan pemijatan kloaka maupun elektroejakulator (Lierz et al., 2013; Kucera & Heidinger, 2018; Frediani et al., 2019). Koleksi semen dengan elektroejakulator direkomendasikan untuk burung berukuran besar seperti burung bangau dan kalkun dengan tingkat keberhasilan sebesar 67%, sementara pemijatan kloaka direkomendasikan untuk burung kecil seperti parkit dengan tingkat keberhasilan 74%
creator: Anggitya Nareswari
creator: Rief Ghulam Satriya Permana
creator: Shafa Adea Puspitadesy
creator: Mualimin Mualimin
creator: Nilahazra Khoirunnisa
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 402
ending page: 407
doi: 10.22146/jsv.107326

Penerapan Sanitasi dan Higiene dalam Produksi Ikan Asap: Implikasi terhadap Keamanan Pangan dan Kesehatan Masyarakat
type: item
description: Keamanan pangan merupakan aspek krusial dalam kesehatan masyarakat, terutama pada produk pangan yang diproses secara tradisional seperti ikan asap. Penelitian ini bertujuan mengevaluasi penerapan sanitasi dan higiene dalam produksi ikan asap di Kota Kendari serta mengkaji dampaknya terhadap keamanan pangan dan kesehatan masyarakat. Penelitian menggunakan desain observasional deskriptif dengan pengambilan sampel secara purposive pada 13 unit produksi ikan asap selama Juli sampai Oktober 2024. Data dikumpulkan melalui observasi langsung dan checklist kepatuhan terhadap standar Badan Pengawas Obat dan Makanan (BPOM). Hasil penelitian menunjukkan kurang dari sebagian produsen (48,4%) yang memastikan peralatan produksi tetap bersih dan terawat secara menyeluruh. Seluruh produsen (100%) menggunakan air bersih selama produksi, namun tanpa fasilitas pembersihan bahan baku yang memadai. Toilet yang memenuhi standar kebersihan hanya ditemukan pada 69,2% lokasi produksi, sementara tidak ada satu pun tempat sampah tertutup (0%). Evaluasi praktik higiene pekerja, didaptkan sebanyak 76,9% pekerja mencuci tangan sebelum dan sesudah bekerja, namun tidak ada pekerja yang mamakai alat pelindung diri (0%). Selain itu, seluruh lokasi produksi tidak memiliki program pembersihan dan sanitasi berkala (0%), dan terdapat hewan peliharaan di sekitar area produksi, yang dapat meningkatkan risiko kontaminasi mikroorganisme patogen. Kondisi ini menunjukkan bahwa industri ikan asap tradisional di Kendari belum memenuhi stadar sanitasi dan higiene yang baik sehingga memerlukan intervensi berbasis kesehatan masyarakat. Diperlukan edukasi kepada produsen mengenai pentingnya sanitasi dan higiene, peningkatan infrastruktur sanitasi, serta pengawasan lebih ketat dari pihak berwenang guna memastikan kepatuhan terhadap standar keamanan pangan. Kata kunci: keamanan pangan, higiene, kesehatan masyarakat, sanitasi, ikan asap
creator: Lymbran Tina
creator: Etih Sudarnika
creator: Yusuf Ridwan
creator: Mirnawati B. Sudarwanto
creator: Herwin Pisestyani
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 408
ending page: 417
doi: 10.22146/jsv.105214

SURVEI TATA LAKSANA TERAPI CAIRAN OLEH DOKTER HEWAN PRAKTISI HEWAN KECIL DI PROVINSI DKI JAKARTA DAN JAWA BARAT
type: item
description: ABSTRACT This survey research was conducted to identify the understanding of small animal veterinarians regarding fluid therapy. Survey was directed to the members of Indonesian Veterinary Medical Association (IVMA) in DKI Jakarta and West Java Province between January 29 and March 25, 2023. The survey questionnaire consisted of 8 questions about the respondent's identity and 39 questions focusing on knowledge about fluid therapy. The sample size using the Slovin method is 290 respondents. Questions focused mainly on knowledge about types, indication, procedures, side effects of fluid therapy, and difficulties when administering fluid therapy. Validity test and reliability test were applied to assess questionnaire effectivity. The results show that almost all respondents had isotonic crystalloid fluid therapy in their clinic (Ringer's Lactate [286/290] and NaCl 0.9% [290/290]). Both fluids are often given either subcutaneously (NaCl 0.9% [81%]; Ringer's Lactate [19%]) or intravenously (NaCl 0.9% [48%]; Ringer's Lactate [46%]). Both fluids also often used also for maintenance (NaCl 0.9% [70%]; Ringer's Lactate [27%]) and for shock resuscitation of dogs (NaCl 0.9% [30%]; Ringer's Lactate [57%]) and cats (NaCl 0.9% [32%]; Ringer's Lactate [57%]). To increase the use of fluid therapy in dogs and cats, veterinarian practitioners' understanding and knowledge regarding fluid therapy is very necessary. ABSTRAK Penelitian ini bertujuan untuk mengidentifikasi pemahaman dokter hewan praktik mengenai terapi cairan. Survei dilakukan kepada anggota Perhimpunan Dokter Hewan Indonesia (PDHI) Provinsi DKI Jakarta dan Jawa Barat dimulai sejak tanggal 29 Januari 2023 hingga 25 Maret 2023. Kuesioner survei dengan terdiri dari 8 pertanyaan tentang identitas responden dan 39 pertanyaan yang difokuskan pada pengetahuan tentang terapi cairan. Perhitungan jumlah responden dilakukan dengan metode Slovin berjumlah 290 responden dengan selang kepercayaan 5%. Pertanyaan difokuskan pada pengetah…
creator: Matilde Surtini Bensa
creator: Raden Harry Soehartono
creator: Riki Siswandi
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 418
ending page: 428
doi: 10.22146/jsv.91727

Detection of Non-Structural Protein and Structural Protein Antibodies in Dairy Cattle Infected with Foot and Mouth Disease (FMD) in Boyolali, Central Java
type: item
description: Penyakit Mulut dan Kuku (PMK) berdampak buruk pada kesejahteraan hewan, kerugian ekonomi, dan pembatasan ekspor ternak dan produk ternak. Hewan yang terinfeksi virus PMK akan menghasilkan antibodi protein non-struktural (NSP) dan protein struktural (SP). Kekebalan terhadap antibodi SP memberikan perlindungan pada hewan setelah infeksi PMK. Adanya antibodi setelah infeksi PMK merupakan indikator penting dalam pelaksanaan program vaksinasi. Penelitian ini bertujuan untuk mendeteksi keberadaan antibodi NSP dan SP serotipe O pada sapi perah. Dua puluh tujuh sapi perah yang terinfeksi PMK dan tidak divaksinasi di Dusun Bendosari, Desa Karangkendal, Kecamatan Musuk, Kabupaten Boyolali digunakan dalam penelitian ini. Deteksi sapi yang terinfeksi PMK menggunakan sampel usap mulut, susu dan serum satu bulan setelah infeksi PMK. Deteksi antibodi NSP dan SP serotipe O menggunakan teknik ELISA dari sampel serum. Serum dikumpulkan setiap bulan sampai enam bulan setelah infeksi PMK. Penelitian ini menunjukkan bahwa antibodi NSP dan SP serotipe O masih terdeteksi pada sapi perah hingga enam bulan setelah infeksi PMK.
creator: Desi Puspita Sari
creator: Sri Handayani Irianingsih
creator: Michael Haryadi Wibowo
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 429
ending page: 437
doi: 10.22146/jsv.99740

ANGKA KEJADIAN DAN KARAKTERISTIK LESI PATOLOGI CALICIVIRUS PADA KUCING (Felis catus) DI VEE VET BANDUNG PERIODE AGUSTUS-SEPTEMBER 2024
type: item
description: Feline calicivirus merupakan penyakit akibat virus yang sangat menular pada kucing. Penyakit ini menyerang saluran pernapasan bagian atas. Feline calicivirus memiliki angka morbiditas yang tinggi dengan gejala yang umumnya serupa dengan penyakit lain. Penelitian ini bertujuan untuk mengetahui angka kejadian serta karakteristik lesi patologi feline calicivirus pada kucing di Vee Vet Bandung periode Agustus – September 2024. Metode penelitian ini dilakukan dengan mengambil data langsung kepada seluruh kucing yang datang ke Vee Vet Bandung periode Agustus-September 2024 dengan keluhan lesi pada mulut dan gangguan pernapasan bagian atas. Pengambilan data dilakukan dengan melakukan uji rapid test dan mendokumentasikan lesi untuk mengetahui bentuk serta lokasi lesi pada kucing dengan kriteria yaitu, hasil positif rapid test feline calicivirus, terdapat lesi pada mulut, dan terdapat gangguan pernapasan bagian atas. Selanjutnya, data akan diolah pada microsoft excel yang kemudian akan dibahas secara deskriptif. Hasil penelitian menunjukan bahwa angka kejadian dari feline calicivirus di Vee Vet Bandung periode Agustus-September 2024 tergolong sangat rendah yaitu 0.05%. Hasil tersebut didapatkan dari 4 pasien terdiagnosa feline calicivirus dengan seluruh populasi kucing yang datang sebanyak 722 pasien. Karaktersitik lesi patologi yang muncul pada seluruh kucing terdiagnosis feline calicivirus adalah seluruh kucing memiliki lesi pada mulut berupa ulser kemerahan khususnya pada bagian lidah dan mukosa mulut serta tidak terjadi nekrosis ataupun edema baik itu di wajah atau pawpad.
creator: Kaila Nurul Arifah
creator: Shafia Khairani
creator: Chrysanti Murad
creator: Yulita Nurul Hikmah Felani
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 438
ending page: 447
doi: 10.22146/jsv.101759

Gambaran Makroskopis dan Histopatologi Hati Broiler yang Diinfeksi Escherichia coli dan Diberi Pakan Terapi Linkomisin-spektinomisin
type: item
description: Antibiotik sebagai senyawa terapi dapat digunakan dengan mencampur ke dalam pakan ternak unggas yang disebut sebagai pakan terapi (medicated feed). Tingginya tingkat resistensi akibat pemberian antibiotik yang tidak tepat menyebabkan sulitnya terapi penyakit yang disebabkan oleh bakteri seperti E. coli. Salah satu cara untuk meningkatkan kemampuan bakterisidal antibiotik ialah dengan mengkombinasikan antibiotik yang tersedia di pasar seperti linkomisin dan spektinomisin. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian pakan terapi linkomisin-spektinomisin terhadap gambaran makroskopis dan mikroskopis hati broiler yang diinfeksi E. coli. Delapan puluh ekor day old chicken (DOC) strain Cobb dibagi kedalam 4 kelompok masing-masing 20 ekor perkelompok secara acak yaitu kontrol positif (KP), kontrol negatif (KN), kelompok perlakuan 1 (K1), kelompok perlakuan 2 (K2) dan dipelihara selama 35 hari. Kelompok KP, K1 dan K2 diinfeksi isolat E. coli melalui 3 rute yaitu intratrakeal sebanyak 0,2 ml, intraperitoneal 0,5 ml dan 0,3 ml oral (109) CFU pada hari ke 16. Kelompok K1 diberi pakan yang telah ditambahkan linkomisin-spektinomisin dengan dosis 5 g/kg selama 7 hari, sedangkan kelompok K2 diberikan selama 14 hari. Kelompok KN dan KP diberi pakan tanpa penambahan kombinasi antibiotik. Ayam yang diinfeksi diamati gejala klinis selama 1-2 hari, seperti bulu kusam dan kasar, ayam terlihat depresi, dan mengalami kelemahan. Semua kelompok perlakuan dinekropsi pada hari ke 35. Hasil skoring lesi hati dianalisis dengan menggunakan uji Kruskal-Wallis sedangkan hasil pengamatan mikroskopis dianalisis secara deskriptif. Hasil penelitian menujukkan kelompok ayam yang diberikan pakan terapi linkomisin-spektinomisin dan diinfeksi E. coli dapat memberikan efek kesembuhan pada organ hati baik secara makroskopis dengan berkurangnya lesi dan secara histopatologi berkurangnya proses inflamasi.
creator: lestari sukma dinullah
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 448
ending page: 455
doi: 10.22146/jsv.91095

Pengembangan Deteksi Parasit Darah Theileria equi dan Babesia caballi secara Otomatis menggunakan Algoritma YOLOv8
type: item
description: Piroplasmosis pada kuda merupakan penyakit parasit darah yang disebabkan oleh Theileria equi dan Babesia caballi, menyebabkan anemia, ikterus, kegagalan organ, serta pembatasan aktivitas kuda, termasuk larangan mengikuti kompetisi. Deteksi piroplasmosis secara rutin masih mengandalkan pemeriksaan mikroskopis ulas darah untuk melihat morfometrik parasitnya sehingga membutuhkan keahlian tinggi dan memerlukan waktu relatif lama. Penelitian ini bertujuan mengembangkan sistem deteksi otomatis parasit T. equi dan B. caballi berbasis algoritma YOLOv8 untuk meningkatkan kecepatan dan akurasi identifikasi mikroskopis. Pengambilan data dilakukan dengan pembuatan preparat ulas darah dari kuda yang terdiagnosis positif piroplasmosis yang telah divalidasi oleh ahli dari Laboratorium Protozoologi SKHB IPB. Preparat diwarnai menggunakan giemsa 10%, dilakukan pengambilan gambar, pembuatan dataset, anotasi data, pelatihan model YOLOv8, serta pengujian performa sistem. Hasil penelitian menunjukkan performa model mendeteksi parasit dengan mAP50 sebesar 69,8%, mAP50-95 sebesar 40,5%, dan kecepatan deteksi 5,4 ms. Evaluasi performa manual menunjukkan akurasi 91%, presisi 98%, recall 92%, dan F1-score 95% dibandingkan pemeriksaan mikroskopis. Penelitian ini menyimpulkan bahwa sistem berbasis YOLOv8 mampu melakukan deteksi T. equi dan B. caballi secara otomatis dengan presisi tinggi dan waktu deteksi cepat sehingga berpotensi digunakan sebagai alat bantu diagnosis piroplasmosis yang lebih efisien.
creator: Ridi Arif
creator: Arifin Budiman Nugraha
creator: Feni Gemala Kedaton
creator: Wishnu Kusumo Agung Erlangga
creator: Bagas Dwi Suryo Wibowo
rights: Copyright (c) 2025 Jurnal Sain Veteriner http://creativecommons.org/licenses/by-sa/4.0
license: http://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-06
publication date: 2025-08-06
volume: 43
number: 2
starting page: 456
ending page: 462
doi: 10.22146/jsv.104069