Pengaruh Hidrogen Peroksida (H2O2) dan Waktu Pengangkutan terhadap Respon Fisiologis Ikan Koi (Cyprinus carpio L.)
Dien Uma Nurina(1*), Sinar Pagi Sektiana(2), I Ketut Sugama(3), Putu Angga Wiradana(4)
(1) Study Program of Utilization of Fisheries Resources, Politeknik Ahli Usaha Perikanan Jakarta, Jl. Aup No.1, RT.1/RW.9, Ps. Minggu, South Jakarta City, Daerah Khusus Ibukota Jakarta 12520, Indonesia
(2) Study Program of Utilization of Fisheries Resources, Politeknik Ahli Usaha Perikanan Jakarta, Jl. Aup No.1, RT.1/RW.9, Ps. Minggu, South Jakarta City, Daerah Khusus Ibukota Jakarta 12520, Indonesia
(3) Study Program of Utilization of Fisheries Resources, Politeknik Ahli Usaha Perikanan Jakarta, Jl. Aup No.1, RT.1/RW.9, Ps. Minggu, South Jakarta City, Daerah Khusus Ibukota Jakarta 12520, Indonesia
(4) Research Group of Biological Health, Study Program of Biology, Faculty of Health and Science, Universitas Dhyana Pura, Jl. Raya Padang Luwih, Dalung, North Kuta, Dalung, Badung Regency, Bali Province 80351, Indonesia
(*) Corresponding Author
Abstract
Ikan koi (Cyprinus carpio L.) merupakan salah satu komoditas ikan hias air tawar introduksi, yang sampai saat ini masih menjadi primadona di pasar internasional dan termasuk jenis ikan hias yang bernilai jual tinggi dengan harga yang relatif stabil. Penelitian ini bertujuan untuk menyelidiki pengaruh pemberian hidrogen peroksida dan waktu pengangkutan terhadap respon fisiologis ikan koi serta interaksi antara hidrogen peroksida dan waktu pengangkutan. Metode yang digunakan Rancangan Acak Lengkap Faktorial dua faktor dan tiga kali ulangan. Ikan koi (panjang 10,70+0,97 cm dan berat 14,45+0,21 gram, n = 540 ekor) digunakan dalam penelitian ini. Faktor pertama (A) yaitu konsentrasi H2O2 sebanyak enam taraf (A1) 0,15 ml/l, (A2) 0,35 ml/l, (A3) 0,55 ml/l, (A4) 0,75 ml/l, (A5) 0,95 ml/l, dan (A6) kontrol. Sedangkan faktor kedua (B) waktu pengangkutan sebanyak tiga taraf yaitu (B1) 10 jam, (B2) 15 jam, (B3) 20 jam. Parameter yang diukur adalah sintasan, kadar kortisol, glukosa, histologi hati, penilaian pasca pengangkutan, dan kualitas air. Hasil penelitian menunjukan bahwa interaksi konsentrasi hidrogen peroksida dan waktu pengangkutan berpengaruh nyata terhadap sintasan, kortisol, dan glukosa. Berdasarkan hasil pengujian, perlakuan terbaik diperoleh pada perlakuan A1B1 dengan konsentrasi hidrogen peroksida sebesar 0,15 ml/l dan waktu pengangkutan 10 jam, yang menghasilkan sintasan sebesar 100 %, kadar kortisol sebesar 0,45 ng/ml, dan kadar glukosa sebesar 56,80 mg/dL, tidak terdapat kerusakan pada jaringan.
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