RAPID CONFIRMATION OF RABIES ANTIGEN IN FORMALIN-FIXED PARAFIN-EMBEDDED TISSUE BY IMMUNOPATHOLOGIC DIAGNOSIS

https://doi.org/10.22146/jsv.8625

R. Wasito(1*), Hastari Wuryastuti(2)

(1) Department of Pathology Faculty of Veterinary Medicine, Gadjah Mada University Yogyakarta
(2) Veterinary Internal Medicine, Faculty of Medicine, Gadjah Mada University, Yogyakarta
(*) Corresponding Author

Abstract


Skunks experimentally infected with street virus rabies
intramuscularly were used to demonstrate rabies viral antigen in
paraffin-embedded tissue by immunopathology diagnosis approach, the
avidin biotin complex (ABC) method. The use of ABC method provides a
simple and sensitive method to localize rabies virus antigen in formalin-
fixed tissues. The results of the present study also demonstrated that a
procedures using formalin-fixed parafin-embeded tissue and stained
immunopathologicalH by the ABC method was an excellent method for
both preservation of morfological details of ceils/tissues and demonstration
of rabies virus antigen.


Keywords


rabies; immunopathologic diagnosis




DOI: https://doi.org/10.22146/jsv.8625

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