Kualitas Spermatozoa Sapi Bali Polled setelah Pemberian Pakan Tersuplementasi Taoge (Phaseolus radiatus L.)

Sri Gustina; Hasbi Hasbi; Herry Sonjaya; Sudirman Baco; Husnul Qhatimah; Wandi Saputra; Mutmainna Mutmainna; Ekayanti Mulyawati Kaiin; Tulus Maulana

doi:10.22146/jsv.86154

Kualitas Spermatozoa Sapi Bali Polled setelah Pemberian Pakan Tersuplementasi Taoge (Phaseolus radiatus L.)

https://doi.org/10.22146/jsv.86154

Sri Gustina^(1*), Hasbi Hasbi⁽²⁾, Herry Sonjaya⁽³⁾, Sudirman Baco⁽⁴⁾, Husnul Qhatimah⁽⁵⁾, Wandi Saputra⁽⁶⁾, Mutmainna Mutmainna⁽⁷⁾, Ekayanti Mulyawati Kaiin⁽⁸⁾, Tulus Maulana⁽⁹⁾

(1) Program Studi Peternakan, Fakultas Peternakan dan Perikanan, Universitas Sulawesi Barat
(2) Departemen Produksi Ternak, Fakultas Peternakan, Universitas Hasanuddin
(3) Departemen Produksi Ternak, Fakultas Peternakan, Universitas Hasanuddin
(4) Departemen Produksi Ternak, Fakultas Peternakan, Universitas Hasanuddin
(5) Departemen Produksi Ternak, Fakultas Peternakan, Universitas Hasanuddin
(6) Departemen Produksi Ternak, Fakultas Peternakan, Universitas Hasanuddin
(7) Departemen Produksi Ternak, Fakultas Peternakan, Universitas Hasanuddin
(8) Research Center for Applied Zoology, Research Organization for Life Science, Badan Riset dan Inovasi Nasional (BRIN)
(9) Research Center for Applied Zoology, Research Organization for Life Science, Badan Riset dan Inovasi Nasional (BRIN)
(*) Corresponding Author

Abstract

The study aimed to increase the sperm quality of polled Bali bulls with the supplementation of bean sprout (Phaseolus radiatus L.) in feeding. This research was used two polled Bali bulls aged 5-6 years old and supplemented with bean sprout 1 kg/bull two times in a week for two months. Semen was collected using artificial vagina method. The evaluation macroscopic of semen was volume while the microscopic evaluation such as motility, viability, abnormality, plasma membrane integrity (PMI), acrosome integrity, and DNA fragmentation. The result of this research showed that semen volume and spermatozoa abnormality of fresh semen after feeding of bean sprouts were not significantly difference (P>0.05) compared to before feeding, but motility and viability were significantly higher (P<0.01). Motility and acrosome integrity of frozen semen were not significantly difference (P>0.05) while viability and PMI were significant higher (P<0.01), abnormality and DNA Fragmentation were significant lower (P<0.01) after feeding compared to before. The research revealed that the supplementation of bean sprouts could increase the motility and viability but had no effect on volume and abnormality of fresh semen. Meanwhile in frozen semen, it increased viability and PMI and decreased abnormality and DNA fragmentation but had no effect on motility and acrosome integrity.

Keywords

bean sprout (Phaseolus radiatus L.); polled Bali bulls; sperm quality

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References

Anastasia, Y.I., Isnaini, N. and Wahjuningsih, S. (2015). Pengaruh level filtrat kecambah kacang hijau dalam pengencer susu skim terhadap kualitas semen cair pejantan sapi Madura pada penyimpanan suhu ruang. Jurnal Ternak Tropika. 16 (2): 55-63. Adiputra, K.D.D., Maulana. T., Kaiin, E.M., Hasbi, H., and Sonjaya, H. (2022). The semen quality of bali and simmental bulls reared in Technical Implementation Unit of Region Artificial Insemination Center at Pucak, South Sulawesi. Advances in Animal and Veterinary Sciences. 10 (12): 2562-2570. Ardhani, F., Mufidah, H., Samsuriati, R. and Putra, H.P. (2020). The storage duration effects of bali bull’s frozen semen at artificial insemination station on plasma membrane integrity, acrosome integrity, and spermatozoa DNA. Jurnal Ilmu Peternakan Terapan. 3 (2): 58-66. Arifiantini, I., Yusuf, T.L. and Graha, N. (2005). Longivitas and recovery rate of freeze fresian Holstein bull semen on different extender. Buletin Peternakan. 29 (2): 53-61. Arifiantini, I. (2012). Teknik Koleksi Dan Evaluasi Semen Pada Hewan. IPB Press, Bogor. Astrid, G.S., Wantouw, B. and Queljoe, E. (2015). Perbedaan antara efek pemberian vitamin c dan vitamin e terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) jantan setelah diberi paparan asap rokok. Jurnal e-Biomedik. 3 (1): 65-71. Baco, S., Zulkharnaim., Malaka, R. and Moekti, G.R. (2020). Polled Bali cattle and potentials for the development of breeding industry in Indonesia. Hasanuddin Journal of Animal Science. 2 (1): 23-33. Badan Standardisasi Nasional. (2017). Semen Beku Sapi Bagian 1. SNI 4869- 1:2017. BSN, Jakarta. Basir, A.A., Hassan, M.S., Buranda, T. and Ferial, E.W. (2013). Pengaruh pemberian nutrisi Phaseolus radiatus L. terhadap tingkat kepadatan spermatozoa Mus musculus L. Biogenesis. 1 (1): 70-73. Bhakat, M., Mohanty, T.K., Raina, V.S., Gupta, A.K., Khan, H.M., Mahapatra, R.K. and Sarkar, M. (2011). Effect of age and season on semen quality parameters in shiwal bulls. Tropical Animal Health Production. 43 (6): 1161-1168. Bochenek, M., Smorag, Z. and Pilch, J. (2001). Sperm chromatin structure assay of bulls qualified for artificial insemination. Theriogenology. 56 (4): 557-67. Bugiwati, S.R.A. (2007). Pertumbuhan dimensi tubuh pedet jantan sapi bali di Kabupaten Bone dan Barru Sulawesi Selatan. Jurnal Sains dan Teknologi. 7: 103–108. Cahya, R.I., Ondho, Y.S. and Setiatin, E.T. (2017). Persentase membran plasma utuh dan tudung akrosom utuh spermatozoa Kambing Peranakan Etawah dalam pengencer yang berbeda. Prosiding Seminar Nasional Politeknik Pembanguan Pertanian, 406-416. Cahyadi, T.R.T., Christiyanto, M. and Setiatin, E.T. (2016). Sperm live-cells and abnormality of etawah grade (PE) bucks supplemented with binahong leaves (Anredera cordifolia (Ten.) Steenis). Animal Agriculture Journal. 5 (3): 23-32. Cameron, A.W.N., Murphy, P.M. and Oidham, C.M. (1988). Nutrition of rams and output of spermatozoa. Proceedings of the Australian of Animal Production. pp 162-165. Celeghini, E.C., Nascimento, J., Raphael, C.F., Andrade, A.F. and Arruda, R.P. (2010). Simultaneous assessment of plasmatic, acrosomal, and mitochondrial membranes in ram sperm by fluorescent probes. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 62 (3): 536-543. D’Occhio, J., Hengstberger, K.J., Tutt, D., Holroyd, R.G., Fordyce, G., Boe-Hansen, G.B. and Johnston, S.D. (2013). Sperm chromatin in beef bulls in tropical environments. Theriogenology. 79 (6): 946-952. Manohara, G.D.I., Normasari, R. and Febianti, Z. (2015). The effect of mung bean (Vigna radiata L) sprout extract on tunica intima-media thickness of aorta abdominalis of male wistar rats induced by physical stress. e-Jurnal Pustaka Kesehatan. 3 (3): 380-385. Diartha, I.W.W, Sudatri, N.W. and Setyawati, I. (2016). The effect of bean sprout extracts and honey to sperm quality of male mice (Mus musculus L.). Jurnal Simbiosis. 4 (1): 1-5. Fazrien, W.A., Herwijanti, E. and Isnaini, N. (2020). Effect of individual variation on fresh and frozen bali bulls semen. Sains Peternakan. 18 (1): 60-65. Garner, D.L. and Hafez, E.S.E. (2000). Spermatozoa and seminal plasma. In Reproduction in Farm Animal. Hafez ESE, Hafez B (Editors). 7th ed. Lippincott Williams & Wilkins, Philadelphia. Gustina, S., Hasbi, H., Sonjaya, H., Baco, S., Toleng, A.L., Mutmainna, M. and Farida, S. (2022). Motility and viability of spermatozoa of polled and horned bali bulls at each stage of the freezing process. Jurnal Ilmu dan Teknologi Peternakan Tropis. 9 (1): 48-54. Handayani, E., Supriatna, I., Tumbelaka, L.I. and Kaiin, E.M. (2021). Comparative analyzis of quality SNI certified and non SNI certified frozen semen. Jurnal Veteriner. 22 (2):207-215. Hasbi, H., Prahesti, K.I., Sonjaya, H., Baco, S., Wildayanti, W. and Gustina, S. (2021). Characteristics of libido and testosterone concentrations of Bali polled and horned bulls. IOP Conference Series: Earth and Environmental Science. 788(012141): 1-6. Hasbi, H., Sonjaya, H., Baco, S., Amalia, R. and Gustina, S. (2021). Characteristics of libido and testosterone concentration of polled and horned Bali bulls after GnRH injection. Jurnal Ilmu Ternak dan Veteriner. 26 (3): 108-114. Hidayanti, N., Sulistiarini, R., Ramadhan, A.M. dan Rijai, L. (2017). Pengaruh pemberian ekstrak taoge (Vigna radiata L.) pada mencit jantan (Mus musculus) terhadap jumlah anak yang dilahirkan. Proceeding of the 5th Mulawarman Pharmaceuticals Conferences, 181-187. Ichwandi. (2004). Performans motilitas, tudung akrosom utuh dan velositas spermatozoa tanpa dan dengan metode ‘swim up’ pasca ‘thawing’ pada semen beku sapi potong. Disertasi, Universitas Diponegoro, Semarang. India Agriculture Ministry. (2014). Compendium of Minimum Standards of Protocol and Standard Operating Procedures for Bovine Breeding. Department of Animal Husbandry, Dairying and Fisheries, India. Indriastuti, R., Ulum, M.F., Arifiantini, R.I. and Purwantara, B. (2020). Individual variation in fresh and frozen semen of Bali bull (Bos sondaicus). Veterinary World. 13 (5): 840-846. Ismaya. (2014). Bioteknologi Inseminasi Buatan pada Sapi dan Kerbau (Bioteknology of Artificial Insemination on Cattle and Bufallo). Gadjah Mada University Press, Yogyakarta. Johnson, W.H. (1997). The significance to bull fertility of morphologically abnormal sperm. The Veterinary Clinics of North America: Food Animal Practice. 13 (2): 255–270. Khezri, A., Narud, B., Stenseth, E.B., Johannisson, A., Myromslien, F.D., Gaustad, A.H., Wilson, R.C., Lyle, R., Morrell, J.M., Kommisrud, E. and Ahmad, R. (2019). DNA methylation patterns vary in boar sperm cells with different levels of DNA fragmentation. BMC Genomics. 20 (1): 1-15. Komariah., Arifiantini, R.I., Aun, M. and Sukmawati, E. (2020). The quality of fresh semen dan frozen semen production madura bulls in different seasons. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan. 8 (1): 5-21. Lemma, A. and Shemsu, T. (2015). Effect of age and breed on semen quality and breeding soundness evaluation of pre-service young bulls. Journal of Reproduction and Infertility. 6 (2): 35-40. Len, J.S., Koh, W.S.D. and Tan, S.X. (2019). The roles of reactive oxygen species and antioxidants in cryopreservation. Bioscience Reports. 39: 1-35. Martianingsih, N., Sudrajat, H.W. dan Darlian, L. (2016). Analisis kandungan protein kecambah kacang hijau (Phaseolus radiatus L.) terhadap variasi waktu perkecambahan. Jurnal Alumni Pendidikan Biologi. 1 (2): 38-42. Martin, G.B., Blache, D., Miller, D.W. and Vercoe, P.E. (2010). Interaction between nutrition and reproduction in the management of the mature male ruminant. Animal. 4 (7):1214-1226. Maruliyananda, C., Hayat, A. and Pidada, I.B.R. (2013). Pengaruh ekstrak etanolik kecambah kacang hijau (Phaseolus radiatus) terhadap jumlah dan morfologi spermatozoa mencit yang terpapar 2-methoxyethanol. Jurnal Ilmiah Biologi FST. 1 (1): 1-10. Momeni., Hamid, R., Mehranjan, Malek, S., Abnosi, M.H., Mahmoodi. and Monireh. (2009). Effects of vitamin e on sperm parameters and reproductive hormones in developing rats treated with para-nonylphenol. Iranian Journal of Reproductive Medicine.7 (3): 111-116. Nabilla, A., Arifiantini, R.I. and Purwantara, B. (2018). Fresh semen quality of Bali bull in productive and non-productive age and determination of cryoprotectant concentration in Tris Egg Yolk extender. Jurnal Veteriner. 19 (2): 242-250. Novita, R. (2020). The influence of long thawing time on the quality of simmental frozen semen microcopically. Tropical Animal Science. 2 (2): 66-73. Nugroho, Y., Susilawati, T. and Wahjuningsih, S. (2015). Kualitas semen sapi Limousin selama pendinginan menggunakan pengencer CEP-2 dengan penambahan berbagai konsentrasi kuning telur dan sari buah jambu biji (Psidium guajava). Jurnal Ternak Tropika. 15 (1): 31-42. Nurcholis., Arifiantini, R.I. and Yamin, M. (2015). Effect of feed bean sprout waste and supplementation omega-3 on production spermatozoa garut rams. Agricola. 5 (2): 133-142. Ostermeier, G.C., Sargean, G.A., Yandell, B.S., Evenson, D.P. and Parrish, J.J. (2001). Relationship of bull fertility to sperm nuclear shape. Journal of Andrology. 22 (4): 595–603. Prabowo, T.A., Arifiantini, R.I., Sajuthi, D. and Saefullah, U. (2016). Development method of livestock sperm DNA damage identification. Jurnal Sain Veteriner. 34 (2): 166-171. Priyanto, L., Arifiantini, R.I. and Yusuf, T.L. (2015). Detection of sperm DNA damage in fresh and frozen semen using toluidine blue staining. Jurnal Veteriner. 16 (1): 48-55. Rangkuti, N.J., Suteky, T. and Putranto, H.D. (2021). Pengaruh waktu pre freezing terhadap kualitas semen beku sapi Bali di UPTD IB Bengkulu. Prosiding Seminar Nasional Pembangunan dan Pendidikan Vokasi Pertanian, 165-176. Rosandi, F.N. and Sjafarjanto, A. (2015). Pengaruh pemberian pakan tambahan tepung bekicot (Achatina fulica) terhadap motilitas dan viabilitas spermatozoa pada mencit (Mus musculus). Vitek. 5: 1-7. Sades, A.M., Isnaini, N. and Wahjuningsih, S. (2016). Pengaruh suplementasi filtrat kecambah kacang hijau (Phaseolus radiatus L.) terhadap kualitas semen sapi simmental dalam pengencer skim milk pada suhu dingin. Jurnal Ternak Tropika. 17 (1): 1-10. Soderquist, L., Janson, L., Larsson, K. and Einarsson, S. (1991). Sperm morphology and fertility in Artificial Insemination bulls. Zentralblatt fur Veterinarmedizin. Reihe A. 38 (7): 534-543. Sorensen, Jr.A.M. (1979). Laboratory manual for animal reproduction. 4th ed. American Press, Boston. Sukmawati, E., Arifiantini, R.I. and Purwantara, B. (2014). Daya tahan spermatozoa terhadap proses pembekuan pada berbagai jenis sapi pejantan unggul. Jurnal Ilmu Ternak dan Veteriner. 19 (3): 168-175. Syarifuddin, N.A. (2021). Daun Kelor Meningkatkan Libido dan Kualitas Sperma Sapi Bali. Bintang Pustaka Madani, Yogyakarta. Thundathil, J., Palasz, A.T., Mapletoft, R.J. and Barth, A.D. (1999). An investigation of the fertilizing characteristics of pyriform-shaped bovine spermatozoa. Animal Reproduction Science. 57 (1): 35–50. Uswa, H.M., Sonjaya, H., Gustina, S. and Hasbi, H. (2022). The concentration of hydrogen peroxide, percentage of motility, viability, plasma membrane integrity, and abnormality of frozen semen of horned and polled Bali bulls. International Journal of Science: Basic and Applied Research. 64 (1): 104-115. Utomo, W.T., Suarsana, I.N. and Suartini, I.G.A.A. (2017). Characteristics of Bali cattle plasma proteins. Jurnal Veteriner. 18 (2):232–238. Wijayanti, P.M., Kirana, A.D. and Indriaswati, T. (2013). Biskuit tauge sebagai “healthy super food” berbasis sumber daya lokal. Prosiding Seminar Nasional Menuju Masyarakat Madani dan Lestari, 555-572. Winarsi H. (2007). Antioksidan Alami dan Radikal Bebas. Kanisius, Yogyakarta. Yahaq, M.A., Ondho, Y.S. and Sutiyono. (2019). Effect of vitamin C addition in the extender on post thawing quality sperm of frozen limousin bull semen. Jurnal Sain Peternakan Indonesia. 14 (4): 380-386. Yuliyantika, Y., Iswari, R.S. and Marianti, A. (2019). Daya proteksi ekstrak tauge kacang hijau terhadap kualitas spermatozoa dan kadar enzim superoksida dismutase mencit yang terpapar transfluthrin. Life Science. 8 (2): 138-149. Zelpina, E., Rosadi, B. and Sumarsono, T. (2012). Kualitas spermatozoa post thawing dari semen beku sapi perah. Jurnal Ilmu-Ilmu Peternakan, 15 (2): 94-102.

DOI: https://doi.org/10.22146/jsv.86154