Antioxidant Effect of Ethanol Extract of Bulbus Dayak Onion (Eleutherine americana) to the Quality of Spermatozoa Exposed by Cigarette Smoke in Rats (Rattus norvegicus)
Demes Chornelia Martantiningtyas(1*), Anni Nurliani(2), Rusmiati Rusmiati(3)
(1) Program Studi Biologi, Fakultas MIPA, Universitas lambung Mangkurat, Kalimantan Selatan
(2) Program Studi Biologi, Fakultas MIPA, Universitas lambung Mangkurat, Kalimantan Selatan
(3) Program Studi Biologi, Fakultas MIPA, Universitas lambung Mangkurat, Kalimantan Selatan
(*) Corresponding Author
Abstract
The toxic substance within the cigarette smoke causing oxidative stress that may damage the quality of spermatozoa. Oxidative stress occurs because the amount of reactive oxygent species and antioxidant produced by the body are not in balanced. One of the alternative approaches of natural antioxidant produced by plant is dayak onion. The bulbus of dayak onion contains steroid, tanin, saphonin, naphtoquinon and flavonoid compound. The aims of this study are to find out the ability of antioxidant from etanol extrat of dayak onion bulbus to increase the quality of spermatozoa affected by smoke and to find out the dose of etanol extrat of dayak onion bulbus that can increase the percentage of viability, morfology, and amount if spermatozoa affected by smoke. In this study, three dose of treatments: 30 mg/kg BW, 60 mg/kg BW, and 90 mg/kb BW were used. The results of dose 30 mg/kg BW, 60 mg/kg BW, and 90 mg/kb BW extended the increased in the normal morfology and amount spermatozoa percentage rather than that of only treated by only the smoke, whereas the viability for
dose of 30 mg/kg BW cannot increased in the percentage of spermatozoa viability after affected by smoke.
Keywords
Full Text:
PDFReferences
Daftar Pustaka
Aitken, J. R., and Roman, S. D. (2008) Antioxidant
system and oxidative stress in testes. Ox. Med.
Cell. Long. 1: 15-24.
Alvarez, J. G. and Storey, B.T. (1995) Differential
incorporation of fatty acids into and
peroxidative loss of fatty acids from
phospholipids of human spermatozoa. Mol
Reprod. Dev. 42: 334-336.
Baker, A. M. and Aitiken, R. J. (2005) Reactive
oxygen species in spermatozoa : methods for
monitoring and significance for the origin of
genetic disease and infertility. Reprod. Biol.
Endrocrinol. 3: 67.
Emmons, C. L. and Peterson, D. M. (1999)
Antioxidant activity and phenolic contents of
oat groats and hulls. Cereal. Chem. 76: 902-
Grivcau, J.F. and Lannou, D. (1997) Reactive
Oksigen Species and Human Spermatozoa:
Physiology and Pathology. Int J Androl, 20 : 61.
Huseein, A., Ayman, A. G., Mohamed and Medhat,
E. (2010) Effect of tobacco smoking on semen
quality in men with subfertility. Uro Today
Internat. J. 10: 3834.
Kuntorini, E. M. dan Astuti, M. D. (2010) Penentuan
aktivitas antioksidan ekstrak etanol bulbus
Demes Chornelia Martantiningtyas et al.
bawang dayak (Eleutherin americana). Sains
dan Terapan Kimia 4: 1.
Kuntorini, E. M. and Laurentius, H. N. (2009)
Structural development and bioactive content
of red bulb plant (Eleutherine americana); a
traditional medicines for local Kalimantan
people. Biodiversitas 11: 102-106.
Pryor, W.A. (1992) Biological effects of cigarette
smoke, wood smoke, and the smoke from
plastics: the use of Electron spin resonance.
Free Radic. Biol. Med. 13: 659–676.
Pryor, W. A., Stone, K., Zang, L.Y. and Bermudez,
E. (1998) Fractionation of aqueous cigarette
tar extracts: fractions that contain the tar radical
cause DNA damage. Chem. Res. Toxicol. 11:
–448.
Riesenfield, A. and. Olivia, H. (1988) Effect nicotin
on the fertility cytology and life span of male
rats. Acta Anat. 131: 171-176.
Robinson, T. (1991) Kandungan Kimia Organik
Tumbuhan Tinggi. Terjemahan Kosasih
Padmawinata. Penerbit ITB, Bandung.
Smith, F. M. (2006) Reproductive Physiology
( A n i m a l S c i e n c e 4 3 1 4 ) .
http://animalsciences.missouri. edu/courses/
/microscope_ slides/ sperm.htm
Diakses tanggal 3 juni 2011
Soares, S. (2009) Cigarette smoking and fertility.
Reprod. Biol. Insight 2: 39-46.
Sorahan, T. P., Lanchasire, R .J., Hulten, M. A., Peck,
I. M. and Stewart, A. M. (1997) Child-hood
cancer and parental use of tobacco, Deaths
from 1953 to 1995. Br. J. Cancer 75: 134-138.
Suhadi, K. dan Arsyad, K. M. (1983) Analisis
sperma. Airlangga Universty Press, Surabaya.
Sukaminingsih, A. (2009) Penurunan jumlah
spermatid pakiten dan spermatid tubulus
seminiferus testis pada mencit (Mus musculus)
yang dipapar asap rokok. J. Biol. 8: 31-35.
Trummer, H., Aberman, H., Josef, H. and Karl, P.
(2002) The impact of cigarette smoking on
human semen parameters and hormones.
Human Reprod. 17: 1554-1559.
Tyassari, H. (2009) Uji aktivitas antidiabetes
ekstrak metanol bulbus bawang dayak
(Eleutherin palmifolia) terhadap tikus putih
jantan yang diinduksi aloksan. Skripsi.
Program S-1, Fakultas Matematika dan Ilmu
Pengetahuan Alam, Universitas Lambung
Mangkurat. Tidak dipublikasikan.
Uotila, J.T., Kirkkola, A. L., Rorarius, M., Tuimala,
R.J. and Metsa-Ketela, R.J. (1994) The total
peroxyl radical-trapping ability of plasma and
cere brospinal fluid in normal and preeclamptic
parturients. Free Rad. Biol. Med. 16: 581-90.
Utami, W., Dai, M. dan Sofiana, Y.R. (2005)
Aktivitas penangkap radikal dengan metode
DPPH serta penetapan kandungan fenol dan
flavonoid dalam ekstrak kloroform, ekstrak etil
asetat, ekstrak etanol daun dewandaru
(Eugenia uniflora L). Pharm. J. Indonesia 6:
-9.
Valavanidis, A., Thomais, V. and Konstantinos, F.
(2009) Tobacco smoke: Involvement of
reactive oxygen species and stable free radicals
in mechanisms of oxidative damage,
carcinogenesis and synergistic effects with
other respirable particles. J. Environ. Res.
Public Health 2: 445–462.
Vine, M.F. (1996) Smoking and male reproduction: a
review. Int. J. Androl. 19: 323-337.
Yardimci, S., Atan, A., Delibasi, T., Sungoruglo, K.
and Guven, M. C. (1997) Long-term effect of
cigarette-smoke exposure on plasma hormon
testosterone luteinizing hormone and folliclestimulating
hormone levels in male rats. Br. J.
Urol 79: 66-69.
DOI: https://doi.org/10.22146/jsv.8111
Article Metrics
Abstract views : 3141 | views : 4252Refbacks
- There are currently no refbacks.
Copyright (c) 2015 Jurnal Sain Veteriner
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Sain Veteriner Indexed by
Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).
Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Jl. Fauna No.2, Karangmalang, Yogyakarta
Phone: 0274-560862
Fax: 0274-560861
Email: jsv_fkh@ugm.ac.id
HP. 0895363078367
Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View My Stats