Antioxidant Effect of Ethanol Extract of Bulbus Dayak Onion (Eleutherine americana) to the Quality of Spermatozoa Exposed by Cigarette Smoke in Rats (Rattus norvegicus)

https://doi.org/10.22146/jsv.8111

Demes Chornelia Martantiningtyas(1*), Anni Nurliani(2), Rusmiati Rusmiati(3)

(1) Program Studi Biologi, Fakultas MIPA, Universitas lambung Mangkurat, Kalimantan Selatan
(2) Program Studi Biologi, Fakultas MIPA, Universitas lambung Mangkurat, Kalimantan Selatan
(3) Program Studi Biologi, Fakultas MIPA, Universitas lambung Mangkurat, Kalimantan Selatan
(*) Corresponding Author

Abstract


The toxic substance within the cigarette smoke causing oxidative stress that may damage the quality of spermatozoa. Oxidative stress occurs because the amount of reactive oxygent species and antioxidant produced by the body are not in balanced. One of the alternative approaches of natural antioxidant produced by plant is dayak onion. The bulbus of dayak onion contains steroid, tanin, saphonin, naphtoquinon and flavonoid compound. The aims of this study are to find out the ability of antioxidant from etanol extrat of dayak onion bulbus to increase the quality of spermatozoa affected by smoke and to find out the dose of etanol extrat of dayak onion bulbus that can increase the percentage of viability, morfology, and amount if spermatozoa affected by smoke. In this study, three dose of treatments: 30 mg/kg BW, 60 mg/kg BW, and 90 mg/kb BW were used. The results of dose 30 mg/kg BW, 60 mg/kg BW, and 90 mg/kb BW extended the increased in the normal morfology and amount spermatozoa percentage rather than that of only treated by only the smoke, whereas the viability for
dose of 30 mg/kg BW cannot increased in the percentage of spermatozoa viability after affected by smoke.


Keywords


Antioxidant, spermatozoa, dayak onion, smoke, rats

Full Text:

PDF


References

Daftar Pustaka

Aitken, J. R., and Roman, S. D. (2008) Antioxidant

system and oxidative stress in testes. Ox. Med.

Cell. Long. 1: 15-24.

Alvarez, J. G. and Storey, B.T. (1995) Differential

incorporation of fatty acids into and

peroxidative loss of fatty acids from

phospholipids of human spermatozoa. Mol

Reprod. Dev. 42: 334-336.

Baker, A. M. and Aitiken, R. J. (2005) Reactive

oxygen species in spermatozoa : methods for

monitoring and significance for the origin of

genetic disease and infertility. Reprod. Biol.

Endrocrinol. 3: 67.

Emmons, C. L. and Peterson, D. M. (1999)

Antioxidant activity and phenolic contents of

oat groats and hulls. Cereal. Chem. 76: 902-

Grivcau, J.F. and Lannou, D. (1997) Reactive

Oksigen Species and Human Spermatozoa:

Physiology and Pathology. Int J Androl, 20 : 61.

Huseein, A., Ayman, A. G., Mohamed and Medhat,

E. (2010) Effect of tobacco smoking on semen

quality in men with subfertility. Uro Today

Internat. J. 10: 3834.

Kuntorini, E. M. dan Astuti, M. D. (2010) Penentuan

aktivitas antioksidan ekstrak etanol bulbus

Demes Chornelia Martantiningtyas et al.

bawang dayak (Eleutherin americana). Sains

dan Terapan Kimia 4: 1.

Kuntorini, E. M. and Laurentius, H. N. (2009)

Structural development and bioactive content

of red bulb plant (Eleutherine americana); a

traditional medicines for local Kalimantan

people. Biodiversitas 11: 102-106.

Pryor, W.A. (1992) Biological effects of cigarette

smoke, wood smoke, and the smoke from

plastics: the use of Electron spin resonance.

Free Radic. Biol. Med. 13: 659–676.

Pryor, W. A., Stone, K., Zang, L.Y. and Bermudez,

E. (1998) Fractionation of aqueous cigarette

tar extracts: fractions that contain the tar radical

cause DNA damage. Chem. Res. Toxicol. 11:

–448.

Riesenfield, A. and. Olivia, H. (1988) Effect nicotin

on the fertility cytology and life span of male

rats. Acta Anat. 131: 171-176.

Robinson, T. (1991) Kandungan Kimia Organik

Tumbuhan Tinggi. Terjemahan Kosasih

Padmawinata. Penerbit ITB, Bandung.

Smith, F. M. (2006) Reproductive Physiology

( A n i m a l S c i e n c e 4 3 1 4 ) .

http://animalsciences.missouri. edu/courses/

/microscope_ slides/ sperm.htm

Diakses tanggal 3 juni 2011

Soares, S. (2009) Cigarette smoking and fertility.

Reprod. Biol. Insight 2: 39-46.

Sorahan, T. P., Lanchasire, R .J., Hulten, M. A., Peck,

I. M. and Stewart, A. M. (1997) Child-hood

cancer and parental use of tobacco, Deaths

from 1953 to 1995. Br. J. Cancer 75: 134-138.

Suhadi, K. dan Arsyad, K. M. (1983) Analisis

sperma. Airlangga Universty Press, Surabaya.

Sukaminingsih, A. (2009) Penurunan jumlah

spermatid pakiten dan spermatid tubulus

seminiferus testis pada mencit (Mus musculus)

yang dipapar asap rokok. J. Biol. 8: 31-35.

Trummer, H., Aberman, H., Josef, H. and Karl, P.

(2002) The impact of cigarette smoking on

human semen parameters and hormones.

Human Reprod. 17: 1554-1559.

Tyassari, H. (2009) Uji aktivitas antidiabetes

ekstrak metanol bulbus bawang dayak

(Eleutherin palmifolia) terhadap tikus putih

jantan yang diinduksi aloksan. Skripsi.

Program S-1, Fakultas Matematika dan Ilmu

Pengetahuan Alam, Universitas Lambung

Mangkurat. Tidak dipublikasikan.

Uotila, J.T., Kirkkola, A. L., Rorarius, M., Tuimala,

R.J. and Metsa-Ketela, R.J. (1994) The total

peroxyl radical-trapping ability of plasma and

cere brospinal fluid in normal and preeclamptic

parturients. Free Rad. Biol. Med. 16: 581-90.

Utami, W., Dai, M. dan Sofiana, Y.R. (2005)

Aktivitas penangkap radikal dengan metode

DPPH serta penetapan kandungan fenol dan

flavonoid dalam ekstrak kloroform, ekstrak etil

asetat, ekstrak etanol daun dewandaru

(Eugenia uniflora L). Pharm. J. Indonesia 6:

-9.

Valavanidis, A., Thomais, V. and Konstantinos, F.

(2009) Tobacco smoke: Involvement of

reactive oxygen species and stable free radicals

in mechanisms of oxidative damage,

carcinogenesis and synergistic effects with

other respirable particles. J. Environ. Res.

Public Health 2: 445–462.

Vine, M.F. (1996) Smoking and male reproduction: a

review. Int. J. Androl. 19: 323-337.

Yardimci, S., Atan, A., Delibasi, T., Sungoruglo, K.

and Guven, M. C. (1997) Long-term effect of

cigarette-smoke exposure on plasma hormon

testosterone luteinizing hormone and folliclestimulating

hormone levels in male rats. Br. J.

Urol 79: 66-69.



DOI: https://doi.org/10.22146/jsv.8111

Article Metrics

Abstract views : 1034 | views : 564

Refbacks

  • There are currently no refbacks.




Copyright (c) 2015 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

  CrossrefROADCOREDimensions  JournalTOCs   


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats