Acute Toxicity Test of Polysaccharides Krestin from Coriolus versicolor Extract with Parameters of Hepatocyte Damages, SGPT and SGOT Enzyme in Mice
Andita Ayu Mandasari(1*), Sri Puji Astuti Wahyuningsih(2), Win Darmanto(3)
(1) Departemen Biologi, Fakultas Sains dan Teknologi, Universitas Airlangga, Surabaya
(2) Departemen Biologi, Fakultas Sains dan Teknologi, Universitas Airlangga, Surabaya
(3) Departemen Biologi, Fakultas Sains dan Teknologi, Universitas Airlangga, Surabaya
(*) Corresponding Author
Abstract
Coriolus versicolor is a mushroom that has polysaccharopeptide krestin (PSK) and polysaccharopeptide (PSP). Many reports showed that polysaccharide krestin as a compound which could reduce mutagen induction, radiation, and development of cancer spontaneusly. However, all substances entering in body could change become toxic depending on dosage. Therefore, this research was aimed to know the effects of PSK on hepatocyte damages, SGPT and SGOT enzymes. Polysaccharide krestin was given by intraperitoneal injection once with treatment groups as follow: K0, was given only saline; P1, was given 80 mg/kg BB PSK dosage; P2, was given 120 mg/ kg BB PSK dosage; P3, was given 160 mg/ kg BB PSK dosage; P4, was given 200 mg/ kg BB PSK dosage; dan P5, was given 240 mg/ kg BB PSK dosage. The results showed that PSK caused hepatic lesions, such as parenchymatous (hydrophic) degeneration of hepatocytes in groups P2 and P3, mild to moderate necrosis in group P4, and moderate to severe necrosis in group P5. Level of SGPT enzyme was not increased, but
level of SGOT enzyme was increased in mice given 160 mg/ kg BB dosage with value 151,62 ± 26,62 IU/ L.
Keywords
Full Text:
PDFReferences
Daftar Pustaka
Cui, J. and Chisti, Y. (2003) Polysaccharapeptides of
Coriolus versicolor: Physiological activity,
Uses, and Production. Biotech. Advances 21:
-122.
Darmanto, W., Pidada, I. B. R. and Prihyantoro, E.
( 2 0 0 4 ) P e m a n f a a t a n e k s t r a k j a m u r
(Polysaccharide krestin) sebagai penghambat
apoptosis dan mencegah munculnya kelainan
janin akibat induksi 2-Methoxyethanol.
Laporan Penelitian. Universitas Airlangga,
Surabaya.
Kobayashi H., Matsunaga, K. and Oguchi, Y. (1995)
Antimetastatic effetcs of PSK (krestin), a
protein-bound polysaccharide obtained from
basidiomycetes: An overview, cancer
epidemiology. Biomarkers Prev. 4: 275-81.
Lazuardi, M. (2008) Struktur histopatologi ginjal
dan hati kambing penderita tripanosomiasisn
pasca pengobatan berenil. Media Peternakan
: 14-21.
Lenaerts, A. J., Johnson, C. M., Marrieta, K. S.,
Gruppo, V., Orme, I. M. (2005) Significant
increase in the levels of liver enzymes in mice
treated with anti-tuberculosis drugs, Int. J.
Antimicrobial Agents 26: 152-158.
Lu, F. C. (1995) Toksikologi dasar: Asas, organ
sasaran, dan penilaian resiko Edisi kedua. UI
Press. Jakarta: 85-102.
Loomis, T. A. (1978) Toksikologi Dasar.
Diterjemahkan oleh Donatus, I. A. Edisi III.
IKIP Semarang Press. Semarang.
Mitruka, B. M. (1981) Clinical biochemical and
hematological reference value in normal
experimental animals and normal humans. 2nd
Ed. Masson Publishing USA. Inc. New York,
USA.
Suarsana, N., Susari, Ni Nyoman Werdi, Wresdiati,
T. dan Suprayogi, A. (2006) Penggunaan
Uji Toksisitas Akut Polisakarida Krestin dari Ekstrak Coriolus versicolor
ekstrak tempe terhadap fungsi hati tikus dalam
kondisi stres. J. Vet. 7: 54-61.
Widjaya, S. (2010) Gangguan faal (fungsi) hati yang
sering ditanyakan oleh penderita. . Diakses
pada tanggal 5 Desember 2010.
Wresdiati, T., Astawan, M. and Hastanti, L. Y. (2006)
Profil imunohistokimia superoksidase
dismutase (SOD) pada jaringan hati tikus
dengan kondisi hiperkolesterolemia. Hayati.
-89,
DOI: https://doi.org/10.22146/jsv.8108
Article Metrics
Abstract views : 2584 | views : 3359Refbacks
- There are currently no refbacks.
Copyright (c) 2015 Jurnal Sain Veteriner
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Sain Veteriner Indexed by
Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).
Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Jl. Fauna No.2, Karangmalang, Yogyakarta
Phone: 0274-560862
Fax: 0274-560861
Email: jsv_fkh@ugm.ac.id
HP. 0895363078367
Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View My Stats