Acute Toxicity Test of Polysaccharides Krestin from Coriolus versicolor Extract with Parameters of Hepatocyte Damages, SGPT and SGOT Enzyme in Mice

https://doi.org/10.22146/jsv.8108

Andita Ayu Mandasari(1*), Sri Puji Astuti Wahyuningsih(2), Win Darmanto(3)

(1) Departemen Biologi, Fakultas Sains dan Teknologi, Universitas Airlangga, Surabaya
(2) Departemen Biologi, Fakultas Sains dan Teknologi, Universitas Airlangga, Surabaya
(3) Departemen Biologi, Fakultas Sains dan Teknologi, Universitas Airlangga, Surabaya
(*) Corresponding Author

Abstract


Coriolus versicolor is a mushroom that has polysaccharopeptide krestin (PSK) and polysaccharopeptide (PSP). Many reports showed that polysaccharide krestin as a compound which could reduce mutagen induction, radiation, and development of cancer spontaneusly. However, all substances entering in body could change become toxic depending on dosage. Therefore, this research was aimed to know the effects of PSK on hepatocyte damages, SGPT and SGOT enzymes. Polysaccharide krestin was given by intraperitoneal injection once with treatment groups as follow: K0, was given only saline; P1, was given 80 mg/kg BB PSK dosage; P2, was given 120 mg/ kg BB PSK dosage; P3, was given 160 mg/ kg BB PSK dosage; P4, was given 200 mg/ kg BB PSK dosage; dan P5, was given 240 mg/ kg BB PSK dosage. The results showed that PSK caused hepatic lesions, such as parenchymatous (hydrophic) degeneration of hepatocytes in groups P2 and P3, mild to moderate necrosis in group P4, and moderate to severe necrosis in group P5. Level of SGPT enzyme was not increased, but
level of SGOT enzyme was increased in mice given 160 mg/ kg BB dosage with value 151,62 ± 26,62 IU/ L.


Keywords


polysaccharide krestin, toxicity, hepatocyte damages, SGPT, SGOT

Full Text:

PDF


References

Daftar Pustaka

Cui, J. and Chisti, Y. (2003) Polysaccharapeptides of

Coriolus versicolor: Physiological activity,

Uses, and Production. Biotech. Advances 21:

-122.

Darmanto, W., Pidada, I. B. R. and Prihyantoro, E.

( 2 0 0 4 ) P e m a n f a a t a n e k s t r a k j a m u r

(Polysaccharide krestin) sebagai penghambat

apoptosis dan mencegah munculnya kelainan

janin akibat induksi 2-Methoxyethanol.

Laporan Penelitian. Universitas Airlangga,

Surabaya.

Kobayashi H., Matsunaga, K. and Oguchi, Y. (1995)

Antimetastatic effetcs of PSK (krestin), a

protein-bound polysaccharide obtained from

basidiomycetes: An overview, cancer

epidemiology. Biomarkers Prev. 4: 275-81.

Lazuardi, M. (2008) Struktur histopatologi ginjal

dan hati kambing penderita tripanosomiasisn

pasca pengobatan berenil. Media Peternakan

: 14-21.

Lenaerts, A. J., Johnson, C. M., Marrieta, K. S.,

Gruppo, V., Orme, I. M. (2005) Significant

increase in the levels of liver enzymes in mice

treated with anti-tuberculosis drugs, Int. J.

Antimicrobial Agents 26: 152-158.

Lu, F. C. (1995) Toksikologi dasar: Asas, organ

sasaran, dan penilaian resiko Edisi kedua. UI

Press. Jakarta: 85-102.

Loomis, T. A. (1978) Toksikologi Dasar.

Diterjemahkan oleh Donatus, I. A. Edisi III.

IKIP Semarang Press. Semarang.

Mitruka, B. M. (1981) Clinical biochemical and

hematological reference value in normal

experimental animals and normal humans. 2nd

Ed. Masson Publishing USA. Inc. New York,

USA.

Suarsana, N., Susari, Ni Nyoman Werdi, Wresdiati,

T. dan Suprayogi, A. (2006) Penggunaan

Uji Toksisitas Akut Polisakarida Krestin dari Ekstrak Coriolus versicolor

ekstrak tempe terhadap fungsi hati tikus dalam

kondisi stres. J. Vet. 7: 54-61.

Widjaya, S. (2010) Gangguan faal (fungsi) hati yang

sering ditanyakan oleh penderita. . Diakses

pada tanggal 5 Desember 2010.

Wresdiati, T., Astawan, M. and Hastanti, L. Y. (2006)

Profil imunohistokimia superoksidase

dismutase (SOD) pada jaringan hati tikus

dengan kondisi hiperkolesterolemia. Hayati.

-89,



DOI: https://doi.org/10.22146/jsv.8108

Article Metrics

Abstract views : 719 | views : 571

Refbacks

  • There are currently no refbacks.




Copyright (c) 2015 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

  CrossrefROADCOREDimensions  JournalTOCs   


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats