Efek Pemberian Maserasi Taoge (Vigna radiata) terhadap Profil Hematologi dan Biokimia Darah Tikus Betina

https://doi.org/10.22146/jsv.73351

andriyanto Andriyanto(1*), Leliana Nugrahaning Widi(2), Rindy Fazni Nengsih(3), Hamdika Yendri Putra(4), Mawar Subangkit(5), Elpita Tarigan(6), Yusa Irarang(7), Aulia Andri Mustika(8), Lina Noviyanti Sutardi(9), Wasmen Manalu(10)

(1) Departemen Anatomi, Fisiologi dan Farmakologi Fakultas Kedokteran Hewan Institut Pertanian Bogor
(2) Departemen Anatomi, Fisiologi dan Farmakologi Fakultas Kedokteran Hewan Institut Pertanian Bogor
(3) Departemen Anatomi, Fisiologi dan Farmakologi Fakultas Kedokteran Hewan Institut Pertanian Bogor
(4) Departemen Anatomi, Fisiologi dan Farmakologi Fakultas Kedokteran Hewan Institut Pertanian Bogor
(5) Departemen Klinik, Reproduksi dan Patologi Fakultas Kedokteran Hewan Universitas Gadjah Mada
(6) Fakultas Kedokteran Hewan Institut Pertanian Bogor
(7) Fakultas Kedokteran Hewan Institut Pertanian Bogor
(8) Departemen Anatomi, Fisiologi dan Farmakologi Fakultas Kedokteran Hewan Institut Pertanian Bogor
(9) Departemen Klinik, Reproduksi dan Patologi Fakultas Kedokteran Hewan Universitas Gadjah Mada
(10) Departemen Anatomi, Fisiologi dan Farmakologi Fakultas Kedokteran Hewan Institut Pertanian Bogor
(*) Corresponding Author

Abstract


Taoge memiliki berbagai manfaat farmakologis yang berpotensi besar untuk meningkatkan kesehatan. Penelitian ini dilakukan untuk mengeksplorasi keamanan dari konsumsi maserasi taoge melalui pengamatan terhadap profil hematologi dan biokimia darah. Sebanyak 15 ekor tikus betina galur Sprague Dawley berumur 8 minggu, memiliki bobot badan 220-230 g, dan belum pernah bunting dikelompokkan secara acak ke dalam 3 kelompok perlakuan (n=5 tikus), yaitu kelompok kontrol (diberi air mineral); kelompok maserasi taoge 1%; dan kelompok maserasi taoge 5%. Pemberian maserasi dilakukan melalui air minum dan dilaksanakan selama 20 hari. Hasil menunjukkan bahwa secara umum perlakuan maserasi taoge tidak memberikan pengaruh nyata (P>0.05) terhadap profil hematologi dan biokimia darah tikus percobaan. Terdapat peningkatan signifikan (P>0.05) pada kadar hemoglobin pada kelompok maserasi taoge 1% dibandingkan dengan kontrol yang lebih tinggi dari nilai normal. Hal yang serupa juga terjadi pada nilai neutrofil yang lebih tinggi dari nilai normal namun tidak berbeda nyata dengan kontrol (p>0.05). Selain keduanya, semua parameter menunjukkan nilai yang tidak berbeda nyata dibandingkan dengan kelompok kontrol dan berada pada rentang nilai normal.. Hal ini mengindikasikan bahwa pemberian maserasi tauge tidak memberikan efek negatif dan toksik pada dosis 1 dan 5% terhadap profil darah serta fungsi organ hati dan ginjal.


Keywords


taoge; maserasi; hematologi; biokimia darah

Full Text:

PDF


References

Bystrom, L.M., Guzman, M.L. dan Rivella, S. (2014). Iron and reactive oxygen species: friends or foes of cancer cells? Antioxidants & Redox Signaling. 20 (12): 1917-1924. Cherng, J.M, Chiang, W. dan Chiang, L.C. (2007). Immunomodulatory activities of edible beans and related constituents from soybean. Food Chemistry. 104 (2007): 613-618. Delwatta, S.L., Gunatilake, M., Baumans, V., Seneviratne, M.D., Dissanayaka, M.L.B., Batagoda, S.S., Udagedara, A.H. dan Walpola, P.B. (2018). Reference values for selected hematological, biochemical and physiological parameters of Sprague-Dawley rats at the Animal House, Faculty of Medicine, University of Colombo, Sri Lanka. Animal Model and Experimental Medicine. 1(4): 250-254. Everds, N.E. (2015). Evaluation of clinical pathology data: correlating changes with other study data. Toxicologic Pathology. 43 (1): 90-97. Filep, J. G. dan Ariel A. (2020). Neutrophil heterogeneity and fate in inflamed tissues: implications for the resolution of inflammation. American Journal of Physiology-Cell Physiology. 319 (3): C510-C532. Ganesan, K. and Baojun, X. (2018). A critical review on phytochemical profile and health promoting effects of mung bean (Vigna radiata). Food Science and Human Wellness. 7 (1): 11-33. He, Q., Su, G., Liu, K., Zhang, F., Jiang, Y., Gao, J., Liu, L., Jiang, Z., Jin, M. dan Xie, H. (2017). Sex-specific reference intervals of hematologic and biochemical analytes in Sprague-Dawley rats using the nonparametric rank percentile method. PLoS One. 12 (12): e0189837. Huang, X., Cai, W. dan Xu, B. (2014). Kinetic changes of nutrients and antioxidant capacities of germinated soybean (Glycine max L.) and mung bean (Vigna radiata L.) with germination time. Food Chemistry. 143 (2014): 268-276. Jom, K.N., Frank, T. dan Engel, K.H. (2011). A metabolite profiling approach to followthe sprouting process of mung beans (Vigna radiata). Metabolomics 7: 102-117. Kang, I., Choi, S., Ha, T.J., Choi, M., Wi, H.R., Lee, B.W. dan Lee, M. (2015). Effects of mung bean (Vigna radiata L.) ethanol extracts decrease proinflammatory cytokine-induced lipogenesis in the KK-Ay diabetes mouse model. Journal of Medicinal Food. 18 (8): 841-849. Li, S., Li, S., Gan, R., Song, F., Kuang, L. dan Li, H. (2013). Antioxidant capacities and total phenolic contents of infusions from 223 medicinal plants. Industrial Crops and Products. 51 : 289-298. Lopes, L.A.R., Martins, M.D.C.C.E., Farias, L.M., Brito, A.K.D.S., Lima, G.M., Carvalho, V.B.L., Pereira, C.F.C., Conde Júnior, A.M., Saldanha, T., Arêas, J.A.G., Silva, K.J.D.E. dan Frota, K.M.G. (2018). Cholesterol-lowering and liver-protective effects of cooked and germinated mung beans (Vigna radiata L.). Nutrients. 10 (7): 821. Manikandaselvi, S., David Raj, C., Aravind, S., Ravikumar, R., Thina-garbabu, R. dan Nandhini, S. (2015). Anti-anemic activity of sprouts of Vigna radiata L. in male albino rats. International Journal of Pharmacy and Pharmaceutical Sciences. 7 (2015): 237-263. McDowell, L.R. (2003). Minerals in Animal And Human Nutrition. 2nd ed. Elsevier Science, Amsterdam. Rivadeneyra-Domínguez, E., Becerra-Contreras, Y., Vázquez-Luna, A., Díaz-Sobac, R. dan Rodríguez-Landa, J.F. (2018). Alterations of blood chemistry, hepatic and renal function, and blood cytometry in acrylamide-treated rats. Toxicology Reports. 5: 1124-1128. Venkateshwarlu, E., Reddy, K.P. dan Dilip, D. (2016). Potential of Vigna radiata (L.) sprouts in the management of inflammation and arthritis in rats: Possible biochemical alterations. Indian Journal of Experimental Biology. 54 (1): 37-43. Wang, P.F., Ye, S.Y. dan Rao, X.Y. (2004). Research progress on the biological activities and functions of mung beans. Journal of Chinese Institute of Food Science and Technology.1: 26. Wang, S,, Rao, P. dan Ye, X. (2009). Isolation and biochemical characterization of a novel leguminous defense peptide with antifungal and antiproliferative potency. Applied Microbiology and Biotechnology. 82 (1): 79-86. WHO. (2010). Traditional herbal remedies for primary health care. Retrieved January 23, 2022, from http://apps.who.int/iris/handle/10665/206024. Wijayanti, H., Nasihun, T. dan Husaana A. (2017). Administration of mung bean extract (Phaseolus radiatus) in increasing Hb and Ferritin level and decreasing malondyaldehide (MDA) level in anaemic rats. Sains Medika. 8 (2): 54-60 Wongsiri, S., Ohshima, T. dan Duangmal, K. (2015). Chemical composition: aminoacid profile and antioxidant activities of germinated mung beans (Vigna radiata), Journal of Food Processing and Preservation. 39 (2015): 1956-1964. Wu, S.J., Wang, J.S., Lin, C.C. dan Chang, C.H. (2001). Evaluation of hepatoprotective activity of legumes. Phytomedicine. 8: 213-219. Yao, Y., Chen, F., Wang, M., Wang, J. dan Ren, G. (2008). Antidiabetic activity of Mung bean extracts in diabetic KK-Ay mice. Journal of Agricultural and Food Chemistry. 56 (19): 8869-8873. Yeap, S.K., Beh, B.K., Ali, N.M., Mohd Yusof, H., Ho, W.Y., Koh, S.P., Alitheen, N.B. dan Long, K. (2014). In vivo anti-stress and antioxidant effects of fermented and germinated mung bean. Biomed Research International. 2014: 694842. Yeap, S.K., Mohd Ali, N., Mohd Yusof, H., Alitheen, N.B., Beh, B.K., Ho, W.Y., Koh, S.P. dan Long, K. (2012). Antihyperglycemic effects of fermented and nonfermented mung bean extracts on alloxan-induced-diabetic mice. Journal of Biomedicine and Biotechnology. 2012: 285430.



DOI: https://doi.org/10.22146/jsv.73351

Article Metrics

Abstract views : 646 | views : 601

Refbacks

  • There are currently no refbacks.




Copyright (c) 2022 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

   CrossrefROADCOREDimensions  JournalTOCs   


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

JJurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats