Profil Reseptor Gonadotropini Releasing Hormone (GnRH) dari Hipothalamus Sapi

Irma Dian Nurani; Claude Mona Airin; Pudji Astuti; Khrisdiana Putri; Bambang Sutrisno

doi:10.22146/jsv.60155

Profil Reseptor Gonadotropini Releasing Hormone (GnRH) dari Hipothalamus Sapi

https://doi.org/10.22146/jsv.60155

Irma Dian Nurani^(1*), Claude Mona Airin⁽²⁾, Pudji Astuti⁽³⁾, Khrisdiana Putri⁽⁴⁾, Bambang Sutrisno⁽⁵⁾

(1) Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(2) Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(3) Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(4) Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(5) Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(*) Corresponding Author

Abstract

Gonadotropine Releasing Hormone (GnRH) is one of the recommended hormones to overcome ovulation problems and it can increase pregnancy rate so that it is used in government programs to increase cattle population in Indonesia, although the results are not yet optimal. Hormone and receptor compatibility is the main key to succesful hormone application while data on the composition of the Indonesian cow GnRH
receptors is not yet known. The purpose of this study was to obtain the composition of GnRH receptors cattle in Indonesia and compare them to the GenBank sequence reference so that is known whether genetic diversity occurs at GnRH receptors cattle in Indonesia. Samples in the form of 3 female cow hypothalamus stored at -800C temperatue freezer. The methods used are mRNA isolation, cDNA synthesis, implification of GnRH gene using Polymerase Chain Reaction (PCR) process by Promoter F and Exon 1 R primers and then elektrophoresis and sent for sequencing. Sequencing product were further aligned with the reference sequences of Bos Taurus
GnRHR mRNA GenBank using the MEGA X program. The results of the analysis found the presence of Single Nucleotide Polymorphism (SNP) in the coding region of 1st cow position 38(A> T), 261(C > T), 342(C >T), 411(C >T) and 495(C > T) and 2nd cow positions 261(C >T). Changes in amino acids were also detected in 1stcow
position 13 (H> L). The presence of SNP was found to indicate genomic variation between individuals at GnRH receptors cattle in Indonesia.

Keywords

Bos Taurus; GnRH; receptors; hypothalamus

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DOI: https://doi.org/10.22146/jsv.60155