Deteksi Bakteri Penyebab Mastitis Subklinis pada Kambing Peranakan Etawah di Kokap, Kulon Progo, Daerah Istimewa Yogyakarta

https://doi.org/10.22146/jsv.57365

Clara Ajeng Artdita(1*), Morsid Andityas(2), Nur Ika Prihanani(3), Yohanes Wawan Budiyanto(4)

(1) Program Studi Kesehatan Hewan, Departemen Teknologi Hayati dan Veteriner, Sekolah Vokasi Universitas Gadjah Mada
(2) Program Studi Kesehatan Hewan, Departemen Teknologi Hayati dan Veteriner, Sekolah Vokasi Universitas Gadjah Mada
(3) Program Studi Kesehatan Hewan, Departemen Teknologi Hayati dan Veteriner, Sekolah Vokasi Universitas Gadjah Mada
(4) Puskeswan Kokap UPTD Puskeswan Wilayah Tengah, Dinas Pertanian dan Pangan Kulon Progo
(*) Corresponding Author

Abstract


Resident in Kokap, Kulon Progo, Yogyakarta Province has another income sector, that is dairy goat (Etawah Crossbreed) farming and they have been joining into farm group, which is called Kelompok Ternak. There are many bacteria that causing dairy goat intra mamary infection, thus the milk production will be contaminated. Two hundred and four (204) milk samples from 103 lactation goat in four farm groups in Kokap, Kulon Progo, Yogyakarta is used in this study. Out of these, 21 samples (10,29%) were detected strong positive (++ and +++) for subclinical mastitis by using California Mastitis Test (CMT). Among this positive result, the next step was bacteria detection. This tests were held in Preclinic Laboratory Animal Health Study Program, Vocational School, UGM. The samples were inoculated in blood agar, then colony was identify by Gram staining. The next steph is biochemical tests using McConkey Agar (MCA) and eosin methylene blue (EMB) as a selective media for Gram negative bacteria, manitol salt agar (MSA) as selective media for Staphylococcus, sugar fermented broth (glucose, lactose, mannitol, maltose, and sacharose), Kigler iron agar (KIA), lysine iron agar (LIA), motility indole ornithin (MIO), and Simmon’s citrate. The result was Staphylococcus aureus (80%), Staphylococcus epidermidis (20%), Escherichia coli (10%), and Klebsiella pneumoniae (10%). 


Keywords


Bacterial detection; Etawah crossbreed goat; Kokap area; Subclinical Mastitis

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References

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DOI: https://doi.org/10.22146/jsv.57365

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