STATUS KLINIS DAN DETEKSI LipL32 SAPI SEROPOSITIF LEPTOSPIROSIS DI KABUPATEN KULON PROGO

https://doi.org/10.22146/jsv.55930

Guntari Titik Mulyani(1), Wayan Tunas Artama(2), Estu Widodo(3*)

(1) Departemen Ilmu Penyakit Dalam Fakultas Kedokteran Hewan Universitas Gadjah Mada
(2) Departemen Biokimia Fakultas Kedokteran Hewan Universitas Gadjah Mada
(3) Bidang Kesehatan Hewan Dinas Pertanian dan Pangan Kabupaten Kulon Progo
(*) Corresponding Author

Abstract


 

 

Leptospirosis is a zoonotic disease caused by Leptospira interrogans. Animals can act as carriers, spread leptospires in urine, and be a source of infection for other animals and humans. In leptospirosis cows can cause abortion, early birth, infertility, decreased milk production and death. The aims of this study was to determine the clinical status and detect the presence of leptospires with Polymerase Chain Reaction (PCR) from urine cows that are expressed as leptospirosis seropositive. A total of 12 cattle seropositive leptospirosis with Microscopic Agglutination Test (MAT) were carried out clinical examinations covering general conditions, pulsus examination, breathing and temperature and organ systems. The urine is collected aseptically, then DNA isolation is carried out using a kit from Genoid. Detection of leptospires in the urine is carried out by detecting the presence of the primary lipoprotein LipL32 making up the Leptospira membrane. The primer was designed with a 21-base forward forward: 5'-TGG ATC TGA TCA ACT ATT ACG-3 ‘containing 38.1% GC with Tm 57.2oC. 22 bases reverse reverse obtained: 5 '-CAC TTC ACC TGG TTT GTA GGT-3' containing GC 45.5% with Tm 62.1oC. Amplification was carried out as many as 40 cycles and continued with electrophoresis to determine the band formed at a wavelength of 506 bp. The results showed that all cows that were positive for leptospirosis with MAT were in a clinically healthy condition. In electrophoresis there are 7 out of 12 positive samples found in the urine leptospira indicated by the formation of a band at 506 bp. From the results of this study it can be concluded that leptospirosis-positive cows do not always show clinical symptoms, but have the potential to excrete leptospires along with urine, so they can act as a source of transmission of leptospirosis to humans, other animals, and the environment.

 


Keywords


Cow; leptospirosis; leptospira

Full Text:

PDF


References

Adler, B., Faine, S., Christopher, W.L., and Chappel, R.J. 1986. Development of and improved selective medium for isolation of leptospires from clinical material. Vet. Microbiol., 12, 377-381. Besung, I. N.K. 2011. Leptospirosis Pada Hewan. Proceedings of 8th National Conggres of Indonesia Association of Clinical Microbiology (PAMKI), November1st – November 3th 2012., Bali, Indonesia Bolin, C.A. 2003. Diagnosis and Control of Bovine Leptospirosis in Proceeding of 6th Western Dairy Management Conference. 12-14, 155-159 Cerqueira, T.B., Athanazio, D.A., Spichle,r A.S., and Seguro, A.C. 2008. Renal involvement in leptospirosisnew insights into pathophysiology and treatment. Braz. J. Infect. Dis., 12, 248–252. Chirataworn, C., Kaewopas, Y., Poorawan, Y., and Suwancharoen, D. 2007. Comparation of A Slide Agglutination Test, Leptotek Dri-dot, and IgM-Elisa with Microscopic Agglutination Test for Leptospirosis Antibody Detection. Research Note, 38 (6), 111-114. Hamond, C., Martin, G., Lilenbaum, W., Pinna, M., and Medeiros, M.A. 2015. Infection by Leptospira spp. In Cattle in Tropical Region, Rio de Janeiro, Brazil. Am. J. Trop. Med. Hyg. 92(1):210. Harkin, K.R., Roshto, Y.M., Sullivan, J.T., Purvis, T.J., and Chengappa, M.M. 2003. Comparison of polymerase chain reaction assay, bacteriologic culture, and serologic testing in assessment of prevalence of urinary shedding of leptospires in dogs. J Am Vet. Med Assoc 222(9): 1230–1233. Morey, RE; Galloway, RL; Bragg, SL; Steigerwalt, AG; Mayer, LW and Levett, PN (2006). Species-specific identification of Leptospiraceae by 16S rRNA gene sequencing. J. Clin. Microbiol., 44: 3510-3516. Mulyani, G.T., Sumiarto, B., Artama, W.T., Hartati, S., Juwari, Sugiwinarsih, S., Putra, H.R.C., Widodo, E. 2016. Kajian Leptospirosis pada Sapi Potong di Daerah Aliran Sungai Progo, Daerah Istimewa Yogyakarta. Jurnal Kedokteran Hewan 10(1):68-71. Ramadhani, T., Yunianto, B. 2012. Reservoir dan Kasus Leptospirosis di Wilayah Kejadian Luar Biasa. J. Kes. Masy. Nas. 6(4), 162-168. Sasaki, D.M., L. Pang, H.P. Minette, C.K. Wakida, W.J. Fujimoto, S.J. Manea, R. Kunioka, and C.R. Middleton. 1993. Active suveillence and risk factor for leptospirosis in Hawai. Am. J. Trop. Med. Hyg. 48(1):35-43. Satbige, A.S., Patil, N.A., Awati, B., and Sandeep, H. 2020. Detection of leptospirosis in the urine of cattle in North Karnataka, South India. Journal of Enthomology and Zoology Studies 8(1):727-729. Slack, A.T., Symonds, M.L., Dohnt, M.F., Smythe, L.D. 2006. Identification of pathogenic Leptospira species by conventional or real-time PCR and sequencing of the DNA gyrase subunit B encoding gene. BMC Microbiol. 6, 95. Vado-Solis, I; Cardenas-Marrufo, MF; Jimwnez-Delgadillo, B; Alzina-Lopez, A; Laviada-Molina, H; Suarez-Solis, V and Zavala-Velazquez, JE (2002). Clinical epidemiological study of leptospirosis in humans and reservoirs in Yucatan, Mexico. Rev. Inst. Med. Trop. Sao Paulo., 44: 335-340. Venkatesha MD, Ramadass P. 2001. Identification of leptospiral isolates by bacterial restriction endonuclease analysis (Brend). Indian J Med. Microbiol.; 19:20322.



DOI: https://doi.org/10.22146/jsv.55930

Article Metrics

Abstract views : 446 | views : 493

Refbacks

  • There are currently no refbacks.




Copyright (c) 2021 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

    CrossrefROADCOREDimensions  JournalTOCs   


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats