Phylogenetic Analysis of Thymidin Kinase Gene Koi HerpesVirus (KHV) in Some Freshwater Fish Aquaculture Centers in East Java
Budi Rianto Wahidi(1*)
(1) Balai Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan Kelas I Surabaya II
(*) Corresponding Author
Abstract
Koi Herpesvirus ( KHV ) is a virus that infects goldfish and koi that resulted in mass death and cause economic and social losses. The use of Polymerase Chain Reaction (PCR) technique to detect KHV was done in nila and gurame. Proof that KHV could infect nila and gurame KHV following genetic variation in each definitive host and spreading area have not been done. Based on these problems, this research was conducted to study the genetic variability and genetic relationships between KHV isolates in some freshwater fish as the definitive host koi, komet , koki, nila and gurame that exist in the area of East Java. The results showed that clinically the gills were pale and hemorrage the end of the tail fin. Electrophoresis results showed that all samples from KHV infected koi, koki, komet, nila and gurame were all positive but the sequencing results for tilapia and carp could not be further identified using the BLAST program. Genetically, koi fish, koki and komet isolates were identical to GenBank isolates code KHV-GZ11 and Indo_0K02SS.
Keywords
Full Text:
PDFDOI: https://doi.org/10.22146/jsv.5430
Article Metrics
Abstract views : 2189 | views : 1998Refbacks
- There are currently no refbacks.
Copyright (c) 2014 Jurnal Sain Veteriner
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Sain Veteriner Indexed by
Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).
Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Jl. Fauna No.2, Karangmalang, Yogyakarta
Phone: 0274-560862
Fax: 0274-560861
Email: jsv_fkh@ugm.ac.id
HP. 0895363078367
Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View My Stats