Mycotoxin Binders Effect on Ovaries of Pregnant Mice Exposed to Zearalenone

https://doi.org/10.22146/jsv.48522

Ragil Angga Prastiya(1*), Abdul Samik(2), Amung Logam Saputro(3)

(1) Faculty of Veterinary Medicine, Universitas Airlangga
(2) Faculty of Veterinary Medicine, Universitas Airlangga
(3) Faculty of Veterinary Medicine, Universitas Airlangga
(*) Corresponding Author

Abstract


Mycotoxin, a secondary metabolite produced by mushroom, has proven to cause various cytotoxic effects to animals. Zearalenone is one type of mycotoxin which is produced by Fusarium graminearum mushroom and has a cytotoxic effect to the reproduction system and cattle productivity by inducing apoptosis in the animal's ovaries, uterus, and placenta. This research tested the potentials of mycotoxin binders in eliminating the effect of zearalenone which can be seen from the expression of caspase 3, the content of malondialdehyde (MDA), and the quantity of corpus luteum in the ovaries of pregnant mice (Mus musculus). Twenty mice were grouped into five different treatments, namely K+ (not exposed to zearalenone and mycotoxin binders), K- (0.1 mg/mouse/day of zearalenone dosage), P1 (0.1 mg/mouse/day of zearalenone dosage and 0.5 mg/mouse/day of mycotoxin binder dosage), P2 (0.1 mg/mouse/day of zearalenone dosage and 1 mg/mouse/day of mycotoxin binders), and P3 (0.1 mg/mouse/day of zearalenone and 2 mg/mouse/day of mycotoxin binders), with 10-days treatment time. The results showed that the potentials of mycotoxin binders could be found on the gradual decline in the expression of caspase 3 in P1, P2 and P3 compared to that in K- (negative control). The results also showed a significant difference in the content of malondialdehyde (MDA) in K- compared to that in K+, P1, P2, and P3. Meanwhile, the quantity of corpus luteum in K- was also starkly different to that in K+, P1, P2, and P3. It can then be concluded that mycotoxin binders are very effective in eliminating the exposure of zearalenone. 


Keywords


Caspase 3; Korpus Luteum; Mycotoxin binders; Malondialdehyde (MDA); Zearalenon

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References

Abbes, S., Z. Ouanes, J.B. Salah-Abbes, Z. Houas, R. Oueslati, H. Bacha, O. Othman. (2006). The Protective Effect of Hydrated Sodium Calcium Aluminosilicate Against Haematological, Biochemical and Pathological Changes Induced by Zearalenone in Mice. Toxicology. 47:567–574.

Albano, E. (2008). Oxidative Mechanisms in The Pathogenesis of Alcoholic Liver Disease. Mol. Asp. Med. 29:9–16.

Avantaggiato, G., M. Solfrizzo, and A. Visconti. (2005). Recent advances on the use of adsorbent materials for detoxification of Fusarium mycotoxins. Food Additives and Contaminants. 22:379–388.

Awoniyi C.A., D. Roberts, and D.N. Veeramachaneni. (1998). Reproductive Sequelae in Female Rats After in Utero and Neonatal Exposure to The Phytoestrogen Genistein. Fertility and Sterility. 70:440-447.

Bouaziz, C., O.S. el Dein, E. el Golli, S. Abid-Essefi, C. Brenner, C. Lemaire and H. Bacha. (2008). Different Apoptotic Pathways Induced by Zearalenone, T-2 Toxin and Ochratoxin A in Human Hepatoma Cells. J.Toxicology 254:19–28.

Bridges, A., R. Lemenager, B. Richert and M. Schutz. (2008). Animal Sciences: Zearalenone Concerns in Reproducing Livestock. Department of Animal Sciences. Purdue U n i v e r s i t y . https://www.extension.purdue.edu/extmedia/ AS/AS-598-W.pdf [12 June, 2015

Döll, S., S. Dänicke, H. Valenta, and G. Flachowsky. (2004). In vitro studies on the evaluation of mycotoxin detoxifying agents for their efficacy on deoxynivalenol and zearalenone. Arch. Anim. Nutr. 58:311-324.

Gajecka, M. (2012). The effect of low-dose experimental zearalenone intoxication on the immunoexpression of estrogen receptors in the ovaries of pre-pubertal bitches. Department of Veterinary Prevention and Feed Hygiene. Faculty of Veterinary Medicine, University of Warmia and Mazury in Olstyn, Oczapowskiego. Poland. J Vet. Sci. 15(4):685-691.

Holme, J.A., M. Gorria, M. Arlt Volker, S. Øvrebø, A. Solhaug, X. Tekpli, E. Landvik Nina, L. Huc, O. Fardel and D. Lagadic-Gossmann. (2007). Different Mechanisms Involved in Apoptosis Following Exposure to Benzo[a]pyrene in F258 and Hepa1c1c7 Cells. Chem. Biol. Interact. 167:41–55.

Kiliańska, Z.M. and A. Miśkiewicz. (2003). Caspases of The Vertebrates – Their Role in Apoptosis. Postępy Biologii Komórki. 30: 129-152.

Kumar, V., RS. Cotran, and S. Robins. (2005). Basic thP athology 7 Ed, Saunders Philadelphia. Indonesian edition: Penerbit Buku Kedokteran EGC. Jakarta, pp. 3-33.

Laurence, DR. and AL. Bacharach. (1993). Evaluation of Drug Activities and Pharmacometrics, Academic Press London and New York 1: pp. 160161.

Maresca, M., and J. Fantini. (2010). Some foodassociated mycotoxins as potential risk factors in humans predisposed to chronic intestinal inflammatory diseases. Toxicon. 56: 282-294.

Obremski, K., M. Gajecki, W. Zwierzchowski, L. Zielonka, I. Otrocka-Domagala, T. Rotkiewicz, A. Mikolajczyk, M. Gajecka and M. Polak. (2003). Influence of Zearalenone on Reproductive System Cell Proliferation in Gilts. Polish Journal of Veterinary Sciences. 6:239–245.

Opletal, L. and B. Šimerda. (2006). Flavanoids in A n i m a l N u t r i t i o n . http://www.vuzv.cz/old/vyziva/ studie29.pdf. [8 March, 2015]

Schoevers E.J., R.R Santos, B. Colenbrander, J. FinkGremmels and B.A. Roelen. (2012). Transgenerational Toxicity of Zearalenone in Pigs. J Reprod Toxicol. 34(1):110-119.

Schollenberger, M., W. Drochner, H.M Müller. (2007). Fusarium Toxins of The Scirpentriol Subgroup: A Review. Mycopathologia 164 (3):101–118.

Sinuhaji, I., B. Siregar, dan Lisnawati. (2013). Ekspresi INK4Ap16 pada Karsinoma Serviks Usia Muda. Departemen Patologi Anatomik. Fakultas Kedokteran Universitas Indonesia. Jakarta. J Indon Med Assoc 63:1.

Tiemann, U., T. Viergutz, L. Jonas, and F. Schneider. (2003). Influence of the mycotoxins - and zearalenol and deoxynivalenol on the cell cycle of cultured porcine endometrial cells. J Reprod. Toxicol. 17(2):209-218



DOI: https://doi.org/10.22146/jsv.48522

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